Kayan Bincike(Zinare Mai Laushi）don Calprotectin
Don amfani da ganewar asali kawai a cikin vitro
Da fatan za a karanta wannan fakitin da aka saka a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da ingancin sakamakon gwaji ba idan akwai wata matsala daga umarnin da ke cikin wannan fakitin.

AN YI NIYYA DA AMFANI
Kayan Bincike na Calprotectin (cal) gwajin colloidal gold immunochromatographic ne don tantance adadin cal daga najasar ɗan adam, wanda ke da mahimmanci ga ganewar asali don cututtukan hanji. Wannan gwajin maganin tantancewa ne. Dole ne a tabbatar da duk samfuran da ke da kyau ta wasu hanyoyin. Wannan gwajin an yi shi ne don amfanin ƙwararrun ma'aikatan kiwon lafiya kawai. A halin yanzu, ana amfani da wannan gwajin don IVD, ba a buƙatar ƙarin kayan aiki.

TAƘAITACCE
Cal wani nau'in heterodimer ne, wanda ya ƙunshi MRP 8 da MRP 14. Yana wanzuwa a cikin ƙwayoyin neutrophils cytoplasm kuma yana bayyana a kan membranes na ƙwayoyin mononuclear. Cal furotin ne mai saurin kamuwa da cuta, yana da tsari mai kyau na kimanin mako guda a cikin najasar ɗan adam, an tabbatar da cewa alama ce ta cututtukan hanji mai kumburi. Kayan aikin gwaji ne mai sauƙi, na gani wanda ke gano cal a cikin najasar ɗan adam, yana da babban ƙarfin ganowa da kuma takamaiman takamaiman aiki. Gwajin da aka yi bisa ƙa'idar amsawar sandwich ta rigakafi mai ƙarfi da fasahar nazarin gwajin immunochromatographic ta zinariya, yana iya bayar da sakamako cikin mintuna 15.

KA'IDUN TSARIN
Wannan tsiri yana da murfin hana cal McAb a yankin gwaji da kuma maganin rigakafi na IgG na akuya a yankin sarrafawa, wanda aka manne shi da membrane chromatography a gaba. Ana shafa murfin da aka yi da colloidal zinariya mai lakabin anti cal McAb da kuma maganin rigakafi na colloidal zinariya mai lakabin IgG a gaba. Lokacin gwada samfurin mai kyau, samfurin cal a cikin samfurin ya haɗu da colloidal zinariya mai lakabin anti cal McAb, kuma ya samar da hadaddun garkuwar jiki, saboda an ba shi damar yin ƙaura tare da tsiri gwajin, haɗin gwiwar cal conjugate yana kamawa ta hanyar murfin anti cal McAb akan membrane kuma ya samar da hadaddun "mai hana cal McAb-cal-colloidal zinariya mai lakabin anti cal McAb", ƙungiyar gwaji mai launi ta bayyana a yankin gwaji. Ƙarfin launi yana da alaƙa mai kyau da abun ciki na cal. Samfurin mara kyau ba ya samar da ƙungiyar gwaji saboda rashin haɗin cal colloidal zinariya mai haɗawa. Ko da cal yana cikin samfurin ko a'a, akwai layin ja a yankin tunani da yankin kula da inganci, wanda ake ɗauka a matsayin ƙa'idodin kasuwanci na ciki.

MASU YIN REAGENTS DA KAYAN DA AKA KAWO
Kayan aikin fakitin 25T:
.Katin gwaji daban-daban an saka shi a cikin jaka da abin cirewa
.Samfurin abubuwan narkewa: sinadaran sune 20mM pH7.4PBS
.Dispette
.Saka fakitin

ANA BUKATAR KAYAN AIKI AMMA BA A BA DA SU BA

Akwatin tattara samfurin, mai ƙidayar lokaci

TARIN SAMFURI DA AJIYEWA
Yi amfani da akwati mai tsabta da za a iya zubarwa don tattara samfurin najasa, sannan a gwada nan take. Idan ba za a iya gwada shi nan take ba, a ajiye a zafin 2-8°C na tsawon awanni 12 ko ƙasa da -15°C na tsawon watanni 4.

Tsarin Gwaji
1. A cire sandar samfurin, a saka a cikin samfurin najasa, sannan a mayar da sandar samfurin, a matse sosai sannan a girgiza sosai, a maimaita aikin sau 3. Ko kuma a yi amfani da samfurin najasa da aka ɗauka kimanin 50mg, sannan a saka a cikin bututun samfurin najasa wanda ke ɗauke da najasa, sannan a matse sosai.
2. Yi amfani da samfurin pipette da za a iya zubarwa, ɗauki samfurin najasa mai siriri daga mai gudawa, sannan a ƙara digo 3 (kimanin 100uL) a cikin bututun samfurin najasa sannan a girgiza sosai, a ajiye a gefe.
3. Cire katin gwaji daga jakar foil, sanya shi a kan teburin matakin sannan ka yi masa alama.
4. Cire murfin daga bututun samfurin kuma jefar da digo biyu na farko na samfurin da aka narkar, ƙara digo 3 (kimanin 100uL) babu kumfa da aka narkar a tsaye kuma a hankali a cikin rijiyar samfurin na katin tare da dispette da aka bayar, fara lokacin.
5. Ya kamata a karanta sakamakon cikin mintuna 10-15, kuma ba shi da inganci bayan mintuna 15.

SAKAMAKON GWAJI DA FASSARAR

Ajiya da kwanciyar hankali
Kayan aikin yana da tsawon rai na watanni 24 daga ranar da aka ƙera shi. Ajiye kayan da ba a yi amfani da su ba a zafin 2-30°C. Kar a buɗe jakar da aka rufe har sai kun shirya yin gwaji.
GARGAƊI DA HANKALI
1. Ya kamata a rufe kayan aikin kuma a kare shi daga danshi.¹.
2. Kar a yi amfani da samfurin da aka sanya shi tsayi ko kuma aka maimaita daskarewa da narkewa don a gwada shi.
3. Samfuran najasa sun yi yawa ko kuma kauri na iya sa samfuran da aka narkar su zama katin gwaji mara kyau, don Allah a sanya centrifuge a cikin samfurin da aka narkar sannan a ɗauki supernatant don gwaji.
4. Rashin aiki yadda ya kamata, ko kuma yawan amfani da samfurin da ba a yi ba, zai iya haifar da karkacewar sakamako.

IYAKA
1. Wannan sakamakon gwajin an yi shi ne kawai don bayanin asibiti, bai kamata ya zama tushen ganewar asali da magani na asibiti ba, ya kamata a yi la'akari da kula da marasa lafiya sosai tare da alamominsa, tarihin likita, sauran gwajin dakin gwaje-gwaje, amsawar magani, ilimin cututtuka da sauran bayanai.².
2. Ana amfani da wannan sinadarin ne kawai don gwajin najasa. Ba lallai ne ya sami sakamako mai kyau ba idan aka yi amfani da shi ga wasu samfura kamar su yau da fitsari da sauransu.