KA'IDA

An rufe membrane na na'urar gwajin da haɗin BSA da 25-(OH)VD a yankin gwaji da kuma antibody na IgG na maganin goat akan yankin sarrafawa. Ana shafa faifan alamar da alamar haske ta antibody na 25-(OH)VD da kuma IgG na zomo a gaba. Lokacin gwada samfurin, 25-(OH)VD a cikin samfurin yana haɗuwa da antibody mai alamar haske ta anti 25-(OH)VD, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin alkiblar takardar sha, lokacin da hadaddun ya wuce yankin gwaji, za a haɗa alamar fluorescent kyauta tare da 25-(OH)VD akan membrane. Yawan 25-(OH)VD yana da alaƙa mara kyau ga siginar fluorescent, kuma yawan 25-(OH)VD a cikin samfurin ana iya gano shi ta hanyar gwajin immunoassay na fluorescence.

Tsarin Gwaji:

Da fatan za a karanta littafin aikin kayan aiki da kuma abin da aka saka a cikin fakitin kafin a gwada.

1. A ajiye dukkan sinadaran da aka haɗa da samfuran a zafin ɗaki.
2. Cire katin gwaji daga jakar foil.
3. Sai a zuba samfurin jini/plasma/jini gaba ɗaya/yatsun hannu 0μL a cikin ruwan, sannan a gauraya sosai
4. Karanta sakamakon gwaji cikin mintuna 15.