Bincike Kit for 25-hydroxy Vitamin D (haske immunochromatographic kima)

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Bincike Kit for 25-hydroxy Vitamin D (haske immunochromatographic kima) Domin a cikin vitro bincike amfani kawai Ka karanta wannan kunshin saka a hankali kafin amfani da kuma tsananin bi umarnin. AMINCI kima sakamakon Ba za'a iya garantin idan akwai wani sabawa daga cikin umarnin a cikin wannan kunshin Saka. Nufin yi bincike Kit for 25-hydroxy Vitamin D (haske immunochromatographic kima) shi ne mai haske immunochromatographic kima domin gwada yawa d ...


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Bincike Kit for 25-hydroxy Vitamin D (haske immunochromatographic kima)
Domin a cikin vitro bincike amfani kawai

Ka karanta wannan kunshin saka a hankali kafin amfani da kuma tsananin bi umarnin. AMINCI kima sakamakon Ba za'a iya garantin idan akwai wani sabawa daga cikin umarnin a cikin wannan kunshin Saka.

Nufin yi
bincike Kit for 25-hydroxy Vitamin D (haske immunochromatographic kima) shi ne mai haske immunochromatographic kima domin gwada yawa ganewa na 25-hydroxy Vitamin D (25- (OH) VD) a cikin mutum magani ko jini, wanda aka yafi amfani ga kimanta da matakan da bitamin D.It ne wani karin ganewar asali reagent.All m samfurin dole ne a tabbatar da wasu methodologies. Wannan gwajin da aka yi nufi ga kiwon lafiya masu sana'a amfani kawai.

TAKAITACCEN
Vitamin D ne a bitamin da kuma shi ne ma mai steroid hormone, yafi ciki har da VD2 da VD3, wanda struction ne sosai kama. Vitamin d3 da D2 suna tuba zuwa 25 hydroxyl bitamin D (ciki har da 25-dihydroxyl bitamin d3 da D2). 25- (OH) VD a cikin jikin mutum, barga struction, babban taro. 25- (OH) VD nuna total adadin bitamin D, da kuma hira ikon bitamin D, don haka 25- (OH) VD aka dauke su mafi kyau nuna alama ga kimantawa da matakin na bitamin D.The bincike Kit dogara ne a kan immunochromatography da kuma iya bayar da sakamakon a cikin mintina 15.

Ka'idar da hanya
The membrane na gwajin na'urar da aka rufi tare da conjugate na BSA kuma 25- (OH) VD a kan gwajin yankin da kuma akuya anti zomo IgG antibody a kan kula da yankin. Alama kushin mai rufi da haske lamba anti 25- (OH) VD antibody da zomo IgG a gaba. Lokacin da gwajin samfurin, 25- (OH) VD a samfurin hada da haske alama anti 25- (OH) VD antibody, da kuma samar da rigakafi cakuda. A karkashin mataki na immunochromatography, da hadaddun kwarara a cikin shugabanci na absorbent takarda, lokacin da hadaddun shige da gwajin yankin, The free kyalli alama za a hade tare 25- (OH) VD a kan membrane.The taro na 25- (OH) VD ne korau hulda ga haske sigina, da kuma maida hankali 25- (OH) VD a samfurin za a iya gano ta hanyar haske immunoassay kima.

Reagents da kuma kayan kawota

25T kunshin da aka gyara :
.Test katin akayi daban-daban tsare pouched da desiccant 25T
.Wani bayani 25T
.B bayani 1
.Package saka 1

Kayan BUKACI AMMA BA TANADIN
Samfurin tarin ganga, da mai židayar lokaci

Samfurin tarin ajiya
1.The samfurori gwada iya zama magani, heparin anticoagulant jini ko EDTA anticoagulant jini.

2.According ga daidaitattun dabaru tattara samfurin. Magani ko jini samfurin za a iya sa a firiji a 2-8 ℃ 7days ga kuma cryopreservation kasa -15 ° C for 6 watanni.
3.All samfurin guje wa daskare-narke hawan keke.

Kima hanya
The gwajin hanya na kayan ganin immunoanalyzer manual. A reagent gwajin hanya ne kamar haka

1.Lay ajiye duk reagents da kuma samfurori zuwa dakin da zazzabi.
2.Open da Fir rigakafi Analyzer (wiz-A101), shigar da kalmar wucewa ta lissafi login bisa ga aiki Hanyar da kayan aiki, da kuma shigar da ganewa dubawa.
3.Scan da dentification code to tabbatar da gwajin abu.
4.Take fitar da gwajin katin daga tsare jakar.
5.Insert da gwajin katin a cikin katin Ramin, duba da QR code, da kuma sanin da gwajin abu.
6.Add 30μL magani ko jini samfurin zuwa wani bayani, da kuma Mix da kyau.
7.Add 50μL B bayani da sama cakuda, da kuma Mix da kyau.
8 .Bar cakuda mintina 15.
9.Add 80μL cakuda zuwa samfurin da kyau na da katin.
10.Click da "daidaitaccen gwajin" button, bayan minti 10, da kayan aiki za ta atomatik gane da gwajin katin, shi zai iya karanta sakamakon daga nuni allo na kayan aiki, da kuma rikodin / bugu da gwajin sakamakon.
11.Refer zuwa wa'azi daga Fir rigakafi Analyzer (wiz-A101).

Ana sa ran dabi'u
25- (OH) VD al'ada range: 30-100ng / ml

An shawarar cewa kowane dakin gwaje-gwaje kafa da kansa al'ada kewayon wakiltar ta haƙuri yawan jama'a.

Gwajin sakamakon da kuma fassarar
.An sama data ne reference tazara da aka kafa domin ganewa data daga wannan kit, kuma an nuna cewa kowane dakin gwaje-gwaje ya kamata kafa reference tazara domin dacewa asibiti muhimmancin yawan jama'a a wannan yankin.

.An maida hankali 25- (OH) VD ne mafi girma fiye da na reference iyaka, da kuma physiological canje-canje ko danniya amsa ya kamata a excluded.Indeed mahaukaci, ya kamata a hada da na asibiti alama ganewar asali.
.An sakamakon wannan hanya ne kawai zartar da reference kewayon kafa ta wannan hanya, da kuma sakamakon ba kai tsaye m da wasu hanyoyin.
.Sauran dalilai kuma iya sa kurakurai a cikin ganewa da sakamakon, ciki har da fasaha dalilai, aiki kurakurai da sauran samfurin dalilai.

Ajiya da kwanciyar hankali
.An kit ne watanni 18 shiryayye-rai daga ranar yi. Ajiye sauran kaya a 2-30 ° C. KADA KA daskare. Kada ka yi amfani hayin ranar karewa.

.Shin ba bude shãfe haske 'yar jakar har ka shirya don yin wani gwajin, da kuma guda-amfani gwajin aka shawara da za a yi amfani da ƙarƙashin da ake buƙata yanayi (zafin jiki 2-35 ℃, zafi 40-90%) cikin 60 mins kamar yadda sauri kamar yadda zai yiwu.
.Sample diluent da ake amfani da nan da nan bayan da aka bude.

Gargadin Kariya
.An kit ya kamata a hatimce da kuma kare da danshi.

.Dukan m samfurori za a inganta da sauran methodologies.
.Dukan samfurori za a bi a matsayin m pollutant.
.Kada ku yi amfani da kare reagent.
.Kuma kada interchange reagents tsakanin kaya tare da daban-daban da yawa ba ..
.Kuma kada sake amfani gwajin cards da wani yarwa na'urorin haɗi.
.Misoperation, wuce kima ko kadan samfurin iya kai ga haifar da sabawa.

L kwaikwayo
.Kamar tare da wani kima yin amfani da linzamin kwamfuta antibodies, da yiwuwar wanzu don kutse ta mutum anti-linzamin kwamfuta antibodies (HAMA) a cikin misali. Samfurori daga marasa lafiya da suka karbi shirye-shirye na monoclonal antibodies ga ganewar asali ko far iya ƙunsar HAMA. Irin wannan samfurori iya haifar da ƙarya kyau ko karya korau sakamakon.

.Wannan gwajin sakamakon da aka sani kawai, domin asibiti reference, kada ku bauta a matsayin kawai dalilin asibiti ganewar asali da kuma magani, da marasa lafiya na asibiti management ya zama m shawara a hade tare da bayyanar cututtuka, likita tarihi, wasu dakin gwaje-gwaje jarrabawa, magani martani, epidemiology da kuma sauran bayanai .
.Wannan reagent ne kawai amfani da magani da kuma jini gwaje-gwaje. Yana iya ba samu cikakken sakamakon lokacin da amfani ga sauran samfurori kamar yau da fitsari da kuma sauransu.

yi halaye

Linearity 5 ng / ml zuwa 120 ng / ml zumunta karkacewa: -15% zuwa + 15%.
Ƙirgar hulda coefficient: (r) ≥0.9900
daidaito A dawo da kudi zai kasance a cikin 85% - 115%.
Repeatability CV≤15%
Bayani dalla-dalla
(Babu wani daga cikin abubuwa a interferent gwada tsoma baki a cikin kima)
Interferent Interferent taro
haemoglobin 200μg / ml
transferrin 100μg / ml
Doki radish peroxidase 2000μg / ml
Vitamin d3 50mg / ml
Vitamin D 50mg / ml

R EFERENCES

1.Hansen JH, et al.HAMA tsangwama tare Murine Monoclonal antibody-Based Immunoassays [J] .Ba fasikai na Sabi Immunoassay, 1993,16: 294-299.
2.Levinson SS.The Nature na Heterophilic antibodies da Aikin a Immunoassay tsangwama [J] .Ba fasikai na Sabi Immunoassay, 1992,15: 108-114.

Key to alamomin amfani:

 t11-1 A vitro bincike Medical Na'ura
 TT-2 manufacturer
 TT-71 Store a 2-30 ℃
 TT-3 ranar karewa
 TT-4 Kada sake amfani
 TT-5 Tsanaki
 TT-6 Shawarci Umarnin Domin Amfani

Xiamen wiz Biotech CO., LTD
Address: 3-4 Floor, NO.16 Building, Bio-likita Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, kasar Sin
Tel: + 86-592-6808278
Fax: + 86- 592-6808279


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