Kit ɗin bincike donCalprotectin(Fluorescence Immunochromatographic Assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai.Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin bincike donCalprotectin(Cal) ya dace da ƙayyadaddun ƙayyadaddun ƙayyadaddun najasar ɗan adam Cal ta hanyar fluorescence immunochromatographic assay, wanda ke da mahimmancin kayan bincike na kayan haɗi don cututtukan hanji mai kumburi. Duk samfuran tabbatacce dole ne a tabbatar da su ta wasu hanyoyin.Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.

TAKAITACCEN
Cal heterodimer ne, wanda ya ƙunshi MRP 8 da MRP 14^[1].Ya wanzu a cikin cytoplasm neutrophils kuma an bayyana shi akan membranes cell mononuclear.Cal yana da sunadaran lokaci mai tsanani, yana da kwanciyar hankali kusan mako guda a cikin najasar ɗan adam, an ƙaddara ya zama alamar cututtukan hanji mai kumburi.^[2-3].Kit ɗin gwajin ƙididdigewa ne mai sauƙi, na gani wanda ke gano Cal a cikin najasar ɗan adam, yana da haɓakar ganowa da ƙaƙƙarfan keɓantacce.Gwajin da aka danganta da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin rigakafin ƙwayoyin cuta guda biyu na sanwicin ka'idar amsawa da fasahar bincike na bincike na fluorescence, wanda zai iya ba da sakamako cikin mintuna 15.

KA'IDAR HANYA
Tsiri yana da anti-Cal shafi antibody a kan gwajin yankin, wanda aka lazimta zuwa membrane chromatography a gaba.Label pad an lullube shi ta hanyar kyalli mai lakabin anti-Cal antibody a gaba.Lokacin gwada ingantaccen samfurin, Cal a cikin samfurin za a iya haɗe shi da mai walƙiya mai lakabin anti-Cal antibody, kuma ya samar da cakudar rigakafi.Yayin da aka ba da izinin cakuda don yin ƙaura tare da tsiri na gwaji, hadaddun Cal conjugate an kama shi ta anti-Cal cover antibody akan membrane kuma ya zama hadaddun.Ƙarfin haske yana da alaƙa da alaƙa da abun ciki na Cal.Ana iya gano Cal a cikin samfurin ta mai nazarin immunoassay na fluorescence.

REAgents DA KAYAN DA AKA BAYAR

Abubuwan kunshin 25T:
Katin gwajin ɗai-ɗai wanda aka sanye da jakar 25T
Samfurin diluents 25T
Kunshin saka 1

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
1.Yi amfani da kwandon tsaftar da za a iya zubarwa don tattara samfurin najasa sabo, kuma a gwada shi nan da nan.Idan ba za a iya gwadawa nan da nan ba, da fatan za a adana a 2-8°C na tsawon kwanaki 3 ko ƙasa da -15°C na tsawon watanni 6.
2.Za a fitar da sandar samfurin, a saka a cikin samfurin najasar, a maimaita aikin sau 3, sai a dauki sassa daban-daban na samfurin najasar a kowane lokaci, sannan a mayar da sandar samfurin a baya, a dunƙule kuma a girgiza sosai, ko amfani da sandar samfurin da aka tsince. kimanin 50mg na najasar samfurin, sannan a saka a cikin bututun samfurin najasa mai ɗauke da samfurin dilution, sannan a dunƙule tam.
3.Yi amfani da samfurin pipette da za a iya zubar da shi, ɗauki samfurin najasa daga majinyacin zawo, sannan a ƙara digo 3 (kimanin 100µL) zuwa bututun samfurin fecal kuma girgiza sosai.

Bayanan kula:
1.A guji daskare-narke hawan keke.
2.Narke samfurori zuwa dakin zafin jiki kafin amfani.

HANYAR ASSAY

Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji.
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6.Cire hula daga tube samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 3 saukad (game da 100uL) babu kumfa diluted samfurin a tsaye da kuma sannu a hankali a cikin samfurin da kyau na katin tare da bayar dispette.
7. Danna maɓallin "gwajin misali", bayan minti 15, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
8. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).

DABI'UN DA AKE SARANSU
Cal <60μg/g
Ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa nasa kewayon al'ada wanda ke wakiltar yawan majinyacinsa.

SAKAMAKON JARRABAWA DA FASSARA
1.Cal a cikin samfurin ya fi 60μg / g, kuma ya kamata ya yi watsi da canjin yanayin yanayin jiki.Sakamakon yana da ban mamaki kuma ya kamata a gano shi tare da alamun asibiti.
2. Sakamakon wannan hanyar yana aiki ne kawai ga jeri na tunani da aka kafa a cikin wannan hanya, kuma babu wani kwatankwacin kai tsaye tare da wasu hanyoyin.
3.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.

AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi.Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C.KAR KA DANKE.Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.

GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban No..
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.

IYAKA
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin.Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA.Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, kula da lafiyar marasa lafiya ya kamata a yi la'akari da shi sosai tare da bayyanar cututtuka, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi ne kawai don gwaje-gwaje na fecal.Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.

HALAYEN YI

NASARA
1.Li, G.&Y.L.Li.Dangatakar da ke tsakanin calcium da cututtuka na asibiti[J].Jarida na Magungunan Magunguna,2007,23(15)
2.Han, W., Xu, JM, et al. Nazarin asibiti na calamine da lactoferrin a cikin marasa lafiya tare da ulcerative colitis [J].Jaridar Sinanci na Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB, et al.Nazarin kan alakar da ke tsakanin fecal calamine da cututtukan hanji mai kumburi[J].Fasahar Kimiyya da Injiniya, 2010-03,10(8)

Maɓalli ga alamomin da aka yi amfani da su:

	In Vitro Diagnostic Medical Na'urar
	Mai ƙira
	Adana a 2-30 ℃
	Ranar Karewa
	Kada a sake amfani
	HANKALI
	Tuntuɓi Umarnin Don Amfani

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