KA'IDA

An shafa membrane na na'urar gwajin da antigen microalbumin a yankin gwaji da kuma antibody IgG antibodies na akuya a yankin sarrafawa. Ana shafa faifan label da fluorescence mai lakabin microalbumin da zomo IgG a gaba. Idan babu albumin a cikin fitsari, antibody monoclonal monoclonal mai lakabin colloidal mai lakabin zinariya anti-Alb akan takardar zinariya ta colloidal zai gudana akan membrane tare da fitsari zuwa layin ganowa, kuma ya haɗu da antigen mai rufi da Alb tare da layi a bayyane. Kuma launin layin ya fi launin layi duhu a yankin sarrafawa (C), wannan sakamako ne mara kyau. Idan fitsarin ya ƙunshi albumin, za su yi gogayya da antigen mai rufi da Alb akan membrane don ɗaure zuwa iyakokin wuraren antibody akan antibody monoclonal mai lakabin zinariya na colloidal anti-Alb. Yayin da adadin albumin a cikin fitsari ke ƙaruwa, gwaji yana ƙaruwa, gwaji

Launin layin zai yi haske da haske. Ana iya gano abun cikin albumin a cikin fitsari ta hanyar kwatanta yankin gano (T) da yankin sarrafawa (C). Yankin kula da inganci (C) da yankin tunani (R) akan kayan aikin koyaushe zasu bayyana yayin gwajin, kuma ba su da alaƙa da kasancewar albumin fitsari. Ana iya amfani da layin kula da (C) da yankin tunani (R) azaman ma'aunin kula da inganci na ciki na kayan aikin.

Tsarin Gwaji:

Da fatan za a karanta littafin aikin kayan aiki da kuma abin da aka saka a cikin fakitin kafin a gwada. A narke samfuran zuwa zafin ɗaki kafin a yi amfani da su.

1. Cire katin gwaji daga jakar foil. Sanya shi a kan saman da aka kwance sannan a yi masa alama.

2. A ɗauki samfurin fitsari da pipette mai yarwa, a jefar da digo biyu na farko na samfurin fitsari. A ƙara digo uku (kimanin 100uL) na fitsarin da ba shi da kumfa a tsakiyar ramin samfurin katin gwaji a tsaye sannan a fara lokacin.

3. Karanta sakamakon a cikin mintuna 10-15. Ba daidai ba ne idan ya wuce mintuna 15.