Me kā mākou ʻenehana alakaʻi i ka manawa like me ko mākou ʻuhane o ka hana hou, ka hui like ʻana, nā pōmaikaʻi a me ka hoʻomohala ʻana, e kūkulu ana mākou i ka wā e hiki mai ana me kāu ʻoihana mahalo no ka Good Quality China HCV Rapid Test Strip / Cassette Enterprise Standard, ʻOiai he hui ʻōpio e ulu nei, ʻaʻole paha mākou ka mea maikaʻi loa, akā ua hoʻāʻo mākou i ka maikaʻi loa no ka lilo ʻana i hoa maikaʻi loa.
Me kā mākou ʻenehana alakaʻi i ka manawa like me ko mākou ʻuhane o ka hana hou, ka hui like ʻana, nā pōmaikaʻi a me ka hoʻomohala ʻana, e kūkulu mākou i kahi wā e hiki mai ana me kāu ʻoihana hanohanoʻAi-HCV-Ns, Kina Hepatitis C Virus, Ke koi mau nei mākou i ka manaʻo hoʻokele o "ʻO ka maikaʻi ka mea mua, ʻo ka ʻenehana ke kumu, ʻoiaʻiʻo a me ka hana hou".
No ka hoʻohana diagnostic in vitro wale nō

E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA

ʻO ka Diagnostic Kit no ka Hepatitis C Virus Antibody (Fluorescence Immunochromatographic Assay) he hōʻike immunochromatographic fluorescence no ka ʻike nui ʻana o ka HCV antibody i loko o ka serum kanaka a i ʻole ka plasma, he mea koʻikoʻi ka waiwai diagnostic auxiliary no ka maʻi me ka hepatitis C. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō

1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
6.Add 20μL serum a plasma hāpana e laʻana diluent, a hui pono..
7.Add 80μL hāpana hāpana no ka laʻana maikaʻi o ke kāleka.
8. E kaomi i ke pihi "ho'āʻo maʻamau", ma hope o nā minuke 15, eʻike pono ka mea hana i ke kāleka ho'āʻo, hiki iā ia ke heluhelu i nā hopena mai ka hōʻikeʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena ho'āʻo.
9. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

HOOLAHA

ʻO ka maʻi maʻi Hepatitis C (HCV) he envelope, hoʻokahi stranded positive sense RNA (9.5 kb) virus no ka ʻohana Flaviviridae. Ua ʻike ʻia nā ʻano genotype ʻeono a me nā ʻano subtypes o HCV. Hoʻokaʻawale ʻia i ka makahiki 1989, ʻike ʻia ʻo HCV ʻo ke kumu nui o ka hoʻopā ʻana i pili i ka maʻi hepatitis non-A, non-B. Hōʻike ʻia ka maʻi me ke ʻano acute a me ka maʻi. ʻOi aku ma mua o 50% o ka poʻe i loaʻa i ka maʻi i hoʻomohala ʻia i ka maʻi hepatitis maʻi hoʻoweliweli me ka cirrhosis ate a me nā maʻi hepatocellular carcinomas. Mai ka hoʻomaka ʻana i ka makahiki 1990 o ka hōʻike ʻana i ka anti-HCV i nā hāʻawi koko, ua hoʻemi nui ʻia ka nui o kēia maʻi i ka poʻe lawe i ka transfusion. Hōʻike nā haʻawina lāʻau lapaʻau i ka nui o ka poʻe i loaʻa i ka maʻi HCV e hoʻomohala i nā antibodies i ka NS5 non-structural protein o ka maʻi. No kēia, ua komo nā hoʻāʻo i nā antigens mai ka ʻāina NS5 o ka genome viral me ka NS3 (c200), NS4 (c200) a me ka Core (c22).

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka antigen HCV ma ka ʻāina hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka papa inoa e ka fluorescence i kapa ʻia ʻo HCV antigen a me ka rabbit IgG ma mua. I ka ho'āʻo ʻana i ka laʻana maikaʻi, hui pū ka antibody HCV i ka laʻana me ka fluorescence i kapa ʻia ʻo HCV antigen, a hana i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i loko o ke kuhikuhi o absorbent pepa, i ka wa paʻakikī i hala i ka ho'āʻo māhele 'āina, ua hui pu me HCV antigen uhi antigen, hana hou complex.HCV antibody pae ua positively correlated me ka fluorescence hoailona, ​​a me ka noonoo ana o ka HCV antibody i ka hāpana hiki ke ike e ka fluorescence immunoassay assay.

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：
. Kāleka hoʻāʻo pakahi i hoʻopaʻa ʻia me kahi desiccant
.Laʻana diluents
.Hoʻokomo pūʻolo

MAKEMAKE IA NA MEA AKA AOLE I HAAWI MAI
Laʻana hōʻiliʻili ipu, manawa
KA OHI A ME KA WAIWAI
1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.
2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana. Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
3.All laʻana e pale i nā pōʻaiapuni freeze-thaw.

HANA HOIKE
E ʻoluʻolu e heluhelu i ka manual operation instrument a me ka hoʻokomo pūʻolo ma mua o ka hoʻāʻo ʻana.
.Keia hopena ho'āʻo mea wale no ka lapaʻau kuhikuhi, aole pono e lawelawe i ke kumu wale no ka maʻi maʻi hōʻoia a me ka lapaʻau, ka poe maʻi hoʻomalu lapaʻau pono e piha noonoo i hui pu ia me kona mau hōʻailona, ​​lapaʻau mōʻaukala, 'ē aʻe hoʻokolohua hoʻokolohua, lapaʻau pane, epidemiology a me nā 'ike.
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NA HIO HANA

HOOLAHA
1. Ma hope o ka hoʻoili ʻia ʻana o ka maʻi hepatitis. In: Moore SB, ed. ʻO nā maʻi maʻi maʻi i hoʻoili ʻia. Alington, VA. Am. ʻO Assoc. Na Waihona Koko, pp. 53-38.
2.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
3.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.
4. Alter HJ., Purcell RH, Holland PV, a me al. (1978) He mea hiki ke hoʻololi i ka maʻi hepatitis non-A, non-B. Lancet I: 459-463.
5.Choo QL, Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Hepatitis C Virus: ka mea kumu kumu nui o ka ma'i hepatitis non-A, non-B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Enzyme linked immunosorbent assay (ELISA): ho'āʻo qualitative o IgG. Immunochemistry 8:871-874.

NA WAIWAI I MANAOIA

HCV-Ab<0.02

Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hana i kona ʻano maʻamau e hōʻike ana i kona heluna maʻi.

HUA HOʻOʻO A ME KA HOʻOKAʻANA

• ʻO ka ʻikepili ma luna ka hopena o ka hoʻāʻo HCV-Ab reagent, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi ʻano o nā waiwai ʻike HCV-Ab kūpono i ka heluna kanaka ma kēia māhele. ʻO nā hualoaʻa ma luna nei no ka kuhikuhi wale nō.
• Hoʻopili wale nā ​​hopena o kēia ʻano i nā pae kuhikuhi i hoʻokumu ʻia ma kēia ʻano, a ʻaʻohe hoʻohālikelike pololei me nā ʻano hana ʻē aʻe.
• Hiki i nā kumu ʻē aʻe ke hana i nā hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA

1. ʻO ka pahu he 18 mahina mālama ola mai ka lā i hana ʻia ai. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. MAI HOOMAIKAI. Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a mākaukau ʻoe e hana i kahi hoʻokolohua, a manaʻo ʻia ka hoʻohana hoʻohana hoʻokahi e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka wela 2-35 ℃, haʻahaʻa 40-90%) i loko o 60 mau minuke me ka wikiwiki.
3. Hoʻohana ʻia ka laʻana diluent ma hope koke o ka wehe ʻana.

OLELO HOOLAHA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
.MAI hoʻohana i ka reagent pau.
.Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻāpana ʻokoʻa No..
.Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.ʻO ka hana hewa ʻole, ka nui a i ʻole ka liʻiliʻi o ka laʻana hiki ke alakaʻi i ka hopena.

LHOOLAHA
.E like me ka ho'āʻo ʻana e hoʻohana ana i nā antibodies ʻiole, aia ka hiki ke hoʻopilikia ʻia e nā anti-mouse antibodies (HAMA) i loko o ka specimen. Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa i ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau. Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​​​hopena maikaʻi ʻole.
Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana