KA HOʻOHANA I MANAʻO ʻIA

Pahu Hōʻailona no Calprotectin(cal) he hoʻāʻo immunochromatographic gula colloidal no ka hoʻoholo semiquantitative o ka cal mai nā feces kanaka, nona ka diagnostic kōkua koʻikoʻi
waiwai no ka maʻi ʻōpū mumū. He mea hoʻāʻo kēia hoʻāʻo. Pono nā hāpana maikaʻi a pau
ua hōʻoia ʻia e nā ʻano hana ʻē aʻe. No ka hoʻohana ʻana o nā loea mālama ola kino wale nō kēia hoʻāʻo.
I kēia manawa, hoʻohana ʻia kēia hoʻāʻo no IVD, ʻaʻole pono nā mea hana keu.
HŌʻULUʻULU MANAʻO
Kale he heterodimer, i haku ʻia me MRP 8 a me MRP 14. Aia ia i loko o ka cytoplasm o nā neutrophils
a hōʻike ʻia ma nā membrane cell mononuclear. He mau protein pae koke ʻo Cal, he paʻa maikaʻi kona
ma kahi o hoʻokahi pule i loko o ka ʻōpala kanaka, ua hoʻoholo ʻia he hōʻailona ia o ka maʻi ʻōpū mumū.
He hoʻāʻo maʻalahi ka pahu hana, ʻike maka semiqualitative e ʻike ana i ke cal i loko o nā ʻōpala kanaka, he kiʻekiʻe kona ʻike ʻana
ʻike a me ke kikoʻī ikaika. ʻO ka hoʻāʻo e pili ana i nā antibodies pālua kikoʻī kiʻekiʻe
ke kumumanaʻo o ka hopena a me nā ʻenehana loiloi immunochromatographic gula, hiki iā ia ke hāʻawi i kahi hopena
i loko o 15 mau minuke.
KUMUHANA O KE KAʻINA HANA
Loaʻa i ke kaha ka uhi anti cal ʻo McAb ma ka ʻāpana hoʻāʻo a me ka antibody IgG anti-rabbit kao ma ka mana.
ʻāpana, kahi i hoʻopaʻa ʻia i ka chromatography membrane ma mua. Ua uhi ʻia ka pad Lable e
ʻO ke gula colloidal i kapa ʻia ʻo anti cal McAb a me ke gula colloidal i kapa ʻia ʻo rabbit IgG antibody ma mua.
I ka hoʻāʻo ʻana i ka hāpana maikaʻi, ua hui pū ʻia ke cal i loko o ka hāpana me ke gula colloidal i kapa ʻia ʻo anti cal McAb,
a hoʻokumu i ka paʻakikī pale, ʻoiai e ʻae ʻia e neʻe ma ke kaha hoʻāʻo, ka cal conjugate
Ua hopu ʻia ka paʻakikī e ka uhi anti cal ʻo McAb ma ka membrane a hana i ka "uhi anti cal
ʻO McAb-cal-colloidal gold i kapa ʻia he anti cal McAb” complex, ua ʻike ʻia kahi hui hoʻāʻo kala ma ka hoʻāʻo
ʻāpana. Pili maikaʻi ka ikaika o ke kala me ka ʻike cal. ʻAʻole kahi hāpana maikaʻi ʻole
e hana i kahi hui hoʻāʻo ma muli o ka loaʻa ʻole o ka colloidal gold conjugate cal complex. ʻAʻohe mea cal
aia i loko o ka hāpana a ʻaʻole paha, aia kahi kaha ʻulaʻula e ʻike ʻia ma ka ʻāpana kuhikuhi a me ka kaohi maikaʻi
ʻāina, kahi i manaʻo ʻia he mau kūlana kūlana kūloko o ka ʻoihana.

ʻO kā mākou hoʻāʻo CAL ka hale hana mua e loaʻa iā CFDA ma Kina. Ua hoʻouna mua mākou i nā ʻāina he nui me nā pane maikaʻi a pau.

Welina mai e hoʻokaʻaʻike mai iā mākou no nā kikoʻī hou aku.