Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic tshuaj ntsuam)

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Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic tshuaj ntsuam) Rau nyob rau hauv vitro diagnostic siv xwb Thov nyeem qhov no pob ntxig ua tib zoo ua ntej yuav siv thiab nruj me ntsis raws li cov lus qhia. Kev ntseeg tau ntawm tshuaj ntsuam tau yuav tsis tau guaranteed hais tias muaj tej yam hloov los ntawm cov lus qhia nyob rau hauv no pob insert. Npaj SIV Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic tshuaj ntsuam) yog ib tug fluorescence immunochromatographic tshuaj ntsuam rau cov quantitative d ...


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Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic tshuaj ntsuam)
Rau nyob rau hauv vitro diagnostic siv xwb

Thov nyeem qhov no pob ntxig ua tib zoo ua ntej yuav siv thiab nruj me ntsis raws li cov lus qhia. Kev ntseeg tau ntawm tshuaj ntsuam tau yuav tsis tau guaranteed hais tias muaj tej yam hloov los ntawm cov lus qhia nyob rau hauv no pob insert.

Npaj SIV
Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic tshuaj ntsuam) yog ib tug fluorescence immunochromatographic tshuaj ntsuam rau cov quantitative nrhiav kom tau ntawm 25-hydroxy Vitamin D (25- (OH) VD) nyob rau hauv tib neeg ntshiab los yog ntshav, uas yog tsuas yog siv los soj ntsuam ntau ntau tus ntawm vitamin D.It yog ib tug pab mob reagent.All zoo qauv yuav tsum tau paub tseeb hais tias los ntawm lwm cov methodologies. Qhov no kuaj no yog npaj rau kws kho mob siv xwb.

NTSIAB
Vitamin D yog ib tug vitamin thiab kuj yog ib tug steroid lawm, mas xws li VD2 thiab VD3, uas nws struction yog heev uas zoo sib xws. Vitamin D3 thiab D2 yog hloov dua siab tshiab rau 25 hydroxyl vitamin D (xws li 25-dihydroxyl vitamin D3 thiab D2). 25- (OH) VD nyob rau hauv tib neeg lub cev, ruaj khov struction, siab concentration. 25- (OH) VD qhia tag nrho cov nyiaj ntawm vitamin D, thiab cov hloov dua siab tshiab muaj peev xwm ntawm vitamin D, yog li 25- (OH) VD yog xav tau qhov zoo tshaj plaws qhia rau ntsuam cov theem ntawm vitamin D.The Diagnostic Kit yog raws li nyob rau hauv immunochromatography thiab yuav muab ib tug tshwm sim tsis pub dhau 15 feeb.

Hauv paus ntsiab lus NTAWM LUB NTSUAM
Lub membrane ntawm qhov kev kuaj ntaus ntawv yog coated nrog lub conjugate ntawm BSA thiab 25- (OH) VD hauv qhov kev xeem cheeb tsam thiab tshis anti luav IgG antibody rau cov kev tswj cheeb tsam. Cim ntaub qhwv to yog coated los ntawm fluorescence cim anti 25- (OH) VD antibody thiab luav IgG nyob rau hauv ua ntej. Thaum kuaj hnoos qeev, 25- (OH) VD nyob rau hauv cov qauv ke nrog fluorescence cim anti 25- (OH) VD antibody, thiab daim ntawv tiv thaiv kab mob sib tov. Nyob rau hauv qhov kev txiav txim ntawm lub immunochromatography, lub complex txaus nyob rau hauv cov kev taw qhia ntawm ntxeem tau dej daim ntawv, thaum complex dhau lub xeem cheeb tsam, Lub free fluorescent marker yuav tau chav nrog 25- (OH) VD rau lub membrane.The concentration ntawm 25- (OH) VD yog tsis zoo correlation rau fluorescence teeb liab, thiab lub concentration ntawm 25- (OH) VD nyob rau hauv cov qauv yuav kuaj tau los ntawm fluorescence immunoassay tshuaj ntsuam.

Reagents THIAB COV NTAUB NTAWV nkag

25T pob Cheebtsam :
.Test card ib lub zuj zus ci pouched nrog ib tug desiccant 25T
.Ib tshuaj 25T
.B tshuaj 1
.Package ntxig 1

NTAUB NTAWV YUAV TSUM TAU TAB SIS TSIS MUAB
Qauv sau thawv, timer

QAUV SAU THIAB CIA
1. kuaj kuaj yuav ua tau ntshiab, heparin anticoagulant ntshav los yog EDTA anticoagulant ntshav.

2.According rau standard hom kev kawm sau cov qauv. Ntshiab los yog ntshav qauv yuav tsum khaws tseg cia hauv tub yees nyob rau ntawm lub 2-8 ℃ rau 7days thiab cryopreservation hauv qab no -15 ° C rau 6 lub hlis.
3.All qauv tsis txhob freeze-thaw mus.

Tshuaj ntsuam NTSUAM
Qhov kev kuaj txheej txheem ntawm lub twj paj nruag saib lub immunoanalyzer phau ntawv no. Lub reagent xeem txoj kev yog raws li nram no

1.Lay tseg tag nrho cov reagents thiab kuaj rau chav tsev kub.
2.Open lub Portable Immune Analyzer (Wiz-A101), nkag mus rau lub account lo lus zais ID nkag mus raws li lub lag luam txoj kev ntsuas, thiab nkag mus rau hauv lub nrhiav kom tau interface.
3.Scan lub dentification code kom paub meej tias cov kev kuaj yam khoom.
4.Take tawm lub kuaj card los ntawm cov ntawv ci hnab.
5.Insert lub xeem daim ntawv mus rau lub daim npav qhov, luam theej duab cov QR code, thiab txiav txim seb lub xeem yam khoom.
6.Add 30μL ntshiab los yog ntshav qauv mus rau A tshuaj, thiab mix zoo.
7.Add 50μL B daws mus rau saum toj no sib tov, thiab mix zoo.
8 .Cia lub sib tov rau 15 feeb.
9.Add 80μL sib tov rau qauv zoo ntawm daim card.
10.Click lub "standard xeem" khawm, tom qab 10 feeb, lub twj paj nruag yuav ntes tus xeem card, nws yuav tau nyeem cov tau los ntawm cov zaub screen ntawm lub twj paj nruag, thiab cov ntaub ntawv / sau lub xeem.
11.Refer rau cov kev qhia ntawv ntawm Portable Immune Analyzer (Wiz-A101).

Xav QHOV TSEEM CEEB
25- (OH) VD cev ntau: 30-100ng / mL

Nws yog pom zoo kom hais tias txhua tus kuaj tsim nws tus kheej cev ntau sawv cev nws tus neeg mob cov pejxeem.

TEST TAU THIAB txhais
.Tus saum toj no cov ntaub ntawv yog cov siv luv tsim rau cov nrhiav kom tau cov ntaub ntawv ntawm cov khoom siv no, thiab nws yog pom tias txhua kuaj yuav tsum tsim kom muaj ib tug siv luv rau cov kev soj ntsuam tseem ceeb ntawm cov pejxeem nyob rau hauv cov cheeb tsam no.

.Tus concentration ntawm 25- (OH) VD yog siab tshaj cov kev siv ntau yam, thiab cov physiological kev hloov los yog teb kev nyuaj siab yuav tsum tau excluded.Indeed txawv txav, yuav tsum muab soj ntsuam cov tsos mob mob.
.Tus tau ntawm cov qauv no yog tsuas muaj feem xyuam rau cov kev siv ntau yam tsim los ntawm no txoj kev, thiab cov kev tshwm sim tsis yog muab nrog lwm txoj kev.
.Other yam tseem ceeb los kuj ua tsis nyob rau hauv kev nrhiav kom tau kev tshwm sim, xws li kev vim li cas, ua hauj lwm uas tsis thiab lwm yam qauv yam.

CIA THIAB STABILITY
.Tus khoom siv yog 18 lub hlis txee-lub neej los ntawm lub hnub ntawm kev siv thiab ua. Cia cov tsis siv cov khoom siv ntawm 2-30 ° C. TSIS TXHOB khov. Tsis txhob siv dhau qhov hnub tas sij hawm.

.Do tsis qhib lub kaw hnab kom txog thaum uas koj npaj txhij mus ua ib txoj kev kuaj, thiab ib leeg-yuav siv cov qhab yog pom tias yuav tsum tau siv nyob rau hauv lub yuav tsum tau puag ncig (kub 2-35 ℃, av noo 40-90%) tsis pub dhau 60 mins kom sai li sai tau.
.Sample diluent yog siv tam sim ntawd tom qab qhib.

CEEB TOOM THIAB KEV CEEV FAJ
.Tus cov khoom siv yuav tsum tau muab zwm tseg thiab kev tiv thaiv tiv thaiv dej.

.All zoo nrws yuav tsum validated los ntawm lwm yam methodologies.
.All nrws yuav tau kho raws li tej zaum pollutant.
.DO TXHOB siv dhau reagent.
.DO TSIS interchange reagents cov khoom siv uas txawv heev tsis muaj ..
.DO TSIS rov qab siv lub xeem phaib thiab tej pov tseg accessories.
.Misoperation, ntau los me me qauv yuav ua tau kom raug hloov.

L imitation
.As nrog rau tej tshuaj ntsuam ua hauj lwm nas antibodies, tau tshwm sim rau cuam los ntawm tib neeg anti-nas tshuaj (HAMA) nyob rau hauv lub tshem me me. Nrws los ntawm cov neeg mob uas tau txais kev npaj ntawm monoclonal tshuaj rau mob los yog kev kho tej zaum yuav muaj HAMA. Tej lub nrws yuav ua cuav zoo los yog tsis muaj tseeb tsis tau.

.Cov kuaj yog tsuas yog rau cov kev soj ntsuam siv, yuav tsum tsis txhob pab raws li lub xwb lub hauv paus rau kev soj ntsuam mob thiab kev kho mob, tus neeg mob soj ntsuam kev tswj yuav tsum tau kev saib xyuas ua ke nrog nws cov tsos mob, kev kho mob yav dhau los, lwm kuaj soj ntsuam, kev kho mob cov lus teb, Epidemiology thiab lwm yam ntaub ntawv .
.Cov reagent yog tsuas yog siv rau cov ntshiab thiab ntshav kev ntsuam xyuas. Nws yuav tsis tau tseeb tshwm sim thaum siv rau lwm yam kuaj xws li cov qaub ncaug thiab zis thiab lwm yam

KEV KAWM CWJ PWM

Linearity 5 ng / mL mus rau 120 ng / mL txheeb ze sib txawv: -15% mus rau + 15%.
Tawm correlation coefficient: (r) ≥0.9900
raug Cov rov qab npaum li cas yuav tsum tsis pub dhau 85% - 115%.
repeatability CV≤15%
Tseem
(Tsis muaj ntawm cov tshuaj nyob rau interferent ntsuam teeb nyob rau hauv lub tshuaj ntsuam)
Interferent Interferent concentration
hemoglobin 200μg / mL
transferrin 100μg / mL
Nees qos liab peroxidase 2000μg / mL
vitamin D3 50MG / mL
vitamin D 50MG / mL

R EFERENCES

1.Hansen JH, thiab al.HAMA Cuam nrog Murine Monoclonal Antibody-Raws li Immunoassays [J] .J ntawm Clin immunoassay, 1993,16: 294-299.
2.Levinson SS.The Xwm ntawm Heterophilic tshuaj thiab lub luag hauj lwm nyob rau hauv immunoassay Cuam [J] .J ntawm Clin immunoassay, 1992,15: 108-114.

Ntsiab rau cim uas siv:

 t11-1 Nyob rau hauv vitro Diagnostic Ntaus Ntawv Kho Mob
 tt-2 Chaw tsim tshuaj paus
 tt-71 Khw muag khoom thaum 2-30 ℃
 tt-3 Tas Hnub
 tt-4 Tsis rov qab siv lub
 tt-5 CEEV FAJ
 tt-6 Nrog Lus Qhia Rau Siv

Xiamen Wiz Biotech CO., LTD
Chaw nyob: 3-4 Pem Teb, NO.16 Building, Bio-kev kho mob Rhiav, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Tuam Tshoj
Tel: + 86-592-6808278
Fax: + 86- 592-6808279


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