Ihe niile anyị na-eme bụ mgbe niile njikọ na echiche anyị "Onye ahịa mbụ, nwee obi ike na nke mbụ, na-etinye uche na nkwakọ ngwaahịa nri na nchekwa gburugburu ebe obibi maka ọnụahịa ahịa China nke afọ 2019Ule Afọ Ime n'etiti 6.0mmNdụ nchekwa ọnwa iri abụọ na anọ, anyị nwere ihe ọmụma gbasara ngwaahịa ọkachamara na ahụmịhe bara ụba na nrụpụta. Anyị na-echekarị na ihe ịga nke ọma gị bụ azụmaahịa anyị!
Ihe anyị na-eme bụ mgbe niile njikọ na echiche anyị "Onye ahịa mbụ, nwee obi ike na nke mbụ, na-etinye uche na nkwakọ ngwaahịa nri na nchekwa gburugburu ebe obibi makaỌkwa Mmetụta nke ule afọ ime na China: Miu 25, Ule Afọ Ime n'etiti 6.0mmNgwa anyị dị elu, njikwa dị mma, ikike nyocha na mmepe na-eme ka ọnụahịa anyị dị ala. Ọnụ ahịa anyị nwere ike ọ gaghị abụ nke kacha ala, mana anyị na-ekwe nkwa na ọ ga-asọmpi nke ọma! Nnọọ ka ịkpọtụrụ anyị ozugbo maka mmekọrịta azụmaahịa n'ọdịnihu na ihe ịga nke ọma n'etiti anyị!
Ngwa Nchọpụta makaHomonụ na-eme ka Luteinizing dịNnyocha immunochromatographic fluorescence (nnwale fluorescence)
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji
Ngwa Nchọpụta maka Luteinizing Hormone (nnwale fluorescence immunochromatographic) bụ nnwale fluorescence immunochromatographic maka nchọpụta ọnụọgụ nke Luteinizing Hormone (LH) na serum ma ọ bụ plasma mmadụ, nke a na-ejikarị eme ihe na nyocha nke ọrụ endocrine pituitary. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.

Nchịkọta

Homonụ Luteinizing (LH) bụ glycoprotein nwere oke molekul dị ihe dị ka Dalton 30,000, nke pituitary anterior na-emepụta. Njupụta nke LH nwere njikọ chiri anya na ovulation nke ovaries, a na-atụkwa anya na elu nke LH ga-abụ awa 24 ruo 36 nke ovulation. Ya mere, enwere ike inyocha oke uru nke LH n'oge okirikiri nsọ iji chọpụta oge kachasị mma ịtụrụ ime. Ọrụ endocrine na-adịghị mma na gland pituitary nwere ike ibute nsogbu nzuzo nke LH. Enwere ike iji ntinye nke LH iji nyochaa ọrụ endocrine pituitary. Ngwa nchọpụta dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji 15.

ỤKPỤRỤ NKE ỤZỌ A

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi LH na mpaghara nnwale ahụ na mgbochi IgG nke ewu na mpaghara njikwa. A na-ekpuchi paadị lable site na iji fluorescence nke ejiri akara anti LH na oke bekee IgG kpuchie tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen LH dị na ihe nlele ahụ na-ejikọta ya na mgbochi LH nke ejiri akara fluorescence mee, ma na-emepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi LH nke ejiri akara anti LH mee, na-emepụta mgbagwoju anya ọhụrụ. Ọkwa LH na-ejikọta nke ọma na mgbaàmà fluorescence, a pụkwara ịchọpụta ntinye nke LH na ihe nlele ahụ site na nnwale immunoassay fluorescence.

Ihe ndị na-eme ka ihe dị nro na ihe ndị e ji mee ihe dị iche iche

Ihe mejupụtara ngwugwu 25T:

. Kaadị ule n'otu n'otu nke ejiri ihe nhicha mmiri 25T kpuchie akpa ahụ
.Ihe ndị na-agbaze ihe nlele
.Ntinye ngwugwu

A CHỌRỌ IHE E JI MEE YA MA E NWEGHỊ YA
Ihe nlele nchịkọta akpa, ihe ngụ oge

Nchịkọta na Nchekwa ihe nlele
1. Ihe nlele ndị a nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2. Dịka usoro ọkọlọtọ si dị, a pụrụ idobe ihe nlele ọbara ma ọ bụ plasma n'ime friji na okpomọkụ 2-8℃ ruo ụbọchị asaa, ebe a na-echekwa ihe nlele ahụ n'okpuru -15°C ruo ọnwa isii.
3. Ihe nlele niile zere usoro oyi kpọnwụrụ akpọnwụ.

Usoro Nnwale

Usoro nnwale nke ngwa ahụ lee akwụkwọ ntuziaka immunoanalyzer. Usoro nnwale reagent bụ dịka ndị a:
1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Mepee Portable Immune Analyzer (WIZ-A101), tinye paswọọdụ akaụntụ dịka usoro ọrụ nke ngwa ahụ si dị, wee tinye interface nchọpụta.
3. Lelee koodu dentification iji kwado ihe nnwale ahụ.
4. Wepụ kaadị ule ahụ n'ime akpa foil ahụ.
5. Tinye kaadị ule ahụ n'ime oghere kaadị ahụ, lelee koodu QR ahụ, wee chọpụta ihe nnwale ahụ.
6. Tinye ihe nlele serum ma ọ bụ plasma 20μL na ihe nlele mmiri, wee gwakọta ya nke ọma.
7. Tinye ihe ngwọta ihe nlele 80μL iji nwalee nke ọma nke kaadị ahụ.
8. Pịa bọtịnụ "ule ọkọlọtọ", mgbe nkeji iri na ise gachara, ngwaọrụ ahụ ga-achọpụta kaadị nnwale ahụ na akpaghị aka, ọ nwere ike ịgụ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, ma dekọọ/bipụta nsonaazụ nnwale ahụ.
9. Lee ntuziaka nke Portable Immune Analyzer (WIZ-A101).

Nsonaazụ na Nkọwa nke Ule

.Data dị n'elu bụ oge ntụaka e guzobere maka data nchọpụta nke ngwa a, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta oge ntụaka maka mkpa ahụike dị mkpa nke ndị bi na mpaghara a.
.Njupụta nke LH dị elu karịa oke ntụaka, a ga-ewepụkwa mgbanwe n'ahụ ma ọ bụ nzaghachi nrụgide. N'ezie, ihe na-adịghị mma kwesịrị ijikọ nchoputa mgbaàmà ahụike.
. Nsonaazụ nke usoro a metụtara naanị oke ntụaka nke usoro a guzobere, nsonaazụ ya adịghịkwa ka nke ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ịkpata mmejọ na nsonaazụ nchọpụta, gụnyere ihe ndị metụtara teknụzụ, mmejọ arụmọrụ na ihe atụ ndị ọzọ.

Nchekwa na nkwụsi ike
1. Ngwa a ga-adịru ọnwa iri na asatọ site na ụbọchị e mepụtara ya. Debe ngwa ndị a na-ejighị na okpomọkụ 2-30°C. EKPERELA FRỊA. Ejila ya karịa ụbọchị njedebe.
2. Emeghela akpa ahụ e mechiri emechi ruo mgbe ị dị njikere ime nnwale, a na-atụkwa aro ka e jiri ule ahụ otu ugboro mee ihe n'okpuru ebe achọrọ (okpomọkụ 2-35℃, iru mmiri 40-90%) n'ime nkeji iri isii ozugbo enwere ike.
3. A na-eji ihe nlele sample ozugbo emepechara ya.

ỊDỌ AKA NA NTỊ NA ỊKPACHICHE
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​na mmiri mmiri.
. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma.
A ga-ewere ihe nlele niile dị ka ihe nwere ike imetọ ihe.
.EJILA ihe na-eme ka ihe ghara ịdị irè.
. EJIGHỊ agbanwe ihe ndị na-emegharị ihe n'etiti ngwa ndị ahụ na ọtụtụ ihe dị iche iche.
.EJILA kaadị ule na ihe ndị ọzọ a na-atụfu atụfu eme ihe ọzọ.
.Ịrụ ọrụ na-ezighi ezi, oke ma ọ bụ obere ihe nlele nwere ike ibute mgbanwe nsonaazụ.

LNṄỤRỤ
Dịka ọ dị na nnwale ọ bụla ejiri ọgwụ mgbochi òké mee, o nwere ike inwe nsogbu site na ọgwụ mgbochi òké mmadụ (HAMA) na ihe nlele ahụ. Ihe nlele sitere n'aka ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchọpụta ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ihe nlele ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ nsonaazụ na-adịghị mma.
.Nsonaazụ ule a bụ naanị maka ntụaka ahụike, ekwesịghị ịbụ naanị ntọala maka nchọpụta na ọgwụgwọ ahụike, njikwa ahụike nke ndị ọrịa kwesịrị ịtụle nke ọma yana mgbaàmà ya, akụkọ ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ.
A na-eji ihe a eme ihe naanị maka ule serum na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dịka ọnụ mmiri na mmamịrị na ihe ndị ọzọ.

ÀGWÀ ÀGWÀ Arụmọrụ

Ntụaka
1. Hansen JH, na ndị ọzọ. Mgbochi HAMA na Mbelata Mgbochi Mgbukpọ nke Dabere na Mgbukpọ Monoclonal [J].J nke Clin Immunoassay, 1993,16:294-299.
2. Levinson SS. Ọdịdị nke Ọgwụ Mgbochi Heterophilic na Ọrụ dị na Mgbochi Immunoassay[J].J nke Clin Immunoassay, 1992,15:108-114.

Isi ihe nnọchianya ejiri mee ihe:

	Ngwaọrụ Ahụike Nchọpụta In Vitro
	Onye nrụpụta
	Chekwaa na okpomọkụ 2-30℃
	Ụbọchị mmebi
	Ejila ya ọzọ
	ỊDỌ AKA NA NTỊ
	Lelee Ntuziaka Maka Ojiji

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