ỤKPỤRỤ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na njikọ nke BSA na 25-(OH)VD na mpaghara nnwale ahụ na antibody IgG anti rabbit na mpaghara njikwa. A na-ekpuchi mpempe akara ahụ site na ihe mgbochi fluorescence mark anti 25-(OH)VD na rabbit IgG tupu oge eruo. Mgbe a na-anwale ihe nlele ahụ, 25-(OH)VD na ihe nlele ahụ na-ejikọta ya na antibody anti 25-(OH)VD akara fluorescence, wee mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, a ga-ejikọta akara fluorescence efu na 25-(OH)VD na membrane ahụ. Ntinye nke 25-(OH)VD bụ njikọ na-adịghị mma maka mgbaàmà fluorescence, enwere ike ịchọpụta ntinye nke 25-(OH)VD na ihe nlele ahụ site na nnwale immunoassay fluorescence.

Usoro Ule:

Biko gụọ akwụkwọ ntuziaka ọrụ ngwa ahụ na ihe e tinyere na ngwugwu tupu ị nwaa ya.

1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Wepụ kaadị ule ahụ n'ime akpa foil ahụ.
3. Tinye 0μL serum/plasma/ọbara dum/mkpịsị aka n'ime mmiri ahụ, ma gwakọta ya nke ọma.
4. Gụọ nsonaazụ ule ahụ n'ime nkeji iri na ise.