Ngwa Nchọpụta(Ọlaedo Colloidal）maka Hormone nke na-akpali follicle
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji

A na-eji ngwa a achọpụta ọkwa nke homonụ na-akpali follicle (FSH) n'ime mmamịrị. Ọ dị mma iji chọpụta ọdịdị nke nsọ nsọ nwanyị.

Nha ngwugwu

Ngwa 1 /igbe, ngwa 10 /igbe, ngwa 25, /igbe, ngwa 50 /igbe.

Nchịkọta

FSH bụ homonụ glycoprotein nke gland pituitary na-ewepụta, ọ nwere ike ịbanye n'ọbara na mmamịrị site na mgbasa ọbara. Maka ụmụ nwoke, FSH na-akwalite ntozu okè nke testicular seminiferous tubule na mmepụta nke sperm, maka ụmụ nwanyị, FSH na-akwalite mmepe na ntozu okè, ma na-ejikọ LH na follicles tozuru okè na-ewepụta estrogen na ovulation, na-etinye aka na nhazi nke nsọ nwanyị nkịtị[1]. FSH na-anọgide na-enwe ọkwa basal kwụsiri ike mgbe niile na ndị mmadụ nkịtị, ihe dị ka 5-20mIU/mL. Mmechi anya ụmụ nwanyị na-abụkarị n'agbata afọ 49 na 54, ma na-adịru nkezi nke afọ anọ ruo ise. N'oge a, n'ihi atrophy nke ovarian, follicular atresia na mmebi, mmepụta estrogen belatara nke ukwuu, ọtụtụ ihe na-akpali akpali nke pituitary gonadotropin secretion, karịsịa ọkwa FSH ga-abawanye nke ukwuu, na-abụkarị 40-200mIU/ml, ma na-anọgide na-enwe ọkwa ahụ n'ime ogologo oge.^[2]Ngwa a dabere na teknụzụ nyocha chromatography colloidal gold immune maka nchọpụta qualitative nke antigen FSH na mmamịrị mmadụ, nke nwere ike inye nsonaazụ n'ime nkeji iri na ise.

Usoro Nnwale
1. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.
2. Tụfuo ihe nlele abụọ mbụ ahụ, tinye ntapụ atọ (ihe dị ka 100μL) enweghị afụ sample kwụ ọtọ ma jiri nwayọ banye n'ime sample nke kaadị ahụ yana dispette enyere, malite oge.
3. A ga-agụ ihe a chọpụtara n'ime nkeji iri ruo iri na ise, ọ gaghịkwa adị irè mgbe nkeji iri na ise gachara.