Kit Tātaritanga mō te 25-hydroxy Huaora D (fluorescence immunochromatographic anga matou)

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Kit Tātaritanga mō te 25-hydroxy Huaora D (fluorescence immunochromatographic anga matou) hoki i roto i vitro whakamahi tātaritanga anake koa lau kōkuhu āta mua ki te whakamahi i tenei mōkihi, me te tino whai i te tohutohu. e kore e taea te whai tika o ngā hua anga matou ki te reira e tetahi ine i te tohutohu i roto i tenei whakauru mōkihi. Te tikanga, USE Tātaritanga Kit mō te 25-hydroxy Huaora D (fluorescence immunochromatographic anga matou) ko te anga matou fluorescence immunochromatographic mo te d ine ...


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Kit Tātaritanga mō te 25-hydroxy Huaora D (fluorescence immunochromatographic anga matou)
hoki i roto i vitro whakamahi tātaritanga anake

koa lau kōkuhu āta mua ki te whakamahi i tenei mōkihi, me te tino whai i te tohutohu. E kore e taea te whai tika o ngā hua anga matou ki te reira e tetahi ine i te tohutohu i roto i tenei whakauru mōkihi.

Te tikanga, USE
Tātaritanga Kit mō te 25-hydroxy Huaora D (fluorescence anga matou immunochromatographic) ko te anga matou fluorescence immunochromatographic mo te kite ine o te 25-hydroxy Huaora D (25 (OH) VD) i roto i te serum wē ranei te tangata, te nuinga te nei whakamahia ki te arotake nga taumata o te huaora D.It ko me whakapumautia he tātaritanga reagent.All tauira pai ngaahi houalotú e te tahi atu tikanga. Tikanga tenei whakamātautau te mo te hauora whakamahi ngaio anake.

WHAKARĀPOPOTOTANGA
Huaora D ko te huaora me te he ano hoki he taiaki pūtaiaki, te nuinga tae VD2 ko VD3, tona struction he tino rite. Huaora D3 ko D2 e tahuri ki te 25 hydroxyl huaora D (tae atu 25-dihydroxyl D3 huaora me D2). 25 (OH) VD i roto i te tinana tangata, struction pūmau, kukū tiketike. 25 whakaata (OH) VD te tapeke o te huaora D, me te kaha faafariuraa o te huaora D, na te whakaaro 25 (OH) VD ki hei te tohu pai mō te aromātai i te taumata o te huaora D.The Diagnostic Kit te hāngai i runga i immunochromatography a taea hoatu he hua i roto i te 15 meneti.

TEFITO'I o te tikanga
pani te kiriuhi o te pūrere whakamātautau kei ki te haumi o Whanonga Kaipāho me 25 (OH) VD i runga i te rohe whakamātautau me te koati koni anti IgG antibody i runga i te rohe mana. E pani tohu papa i te tohu fluorescence anti 25 (OH) VD antibody me te koni IgG i mua. A, no te whakamatautau tauira, 25 (OH) VD i roto i te tauira ngā ki fluorescence tohua anti 25 (OH) VD antibody, ka hanga i ranunga ārai. I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka haere matatini te rohe whakamātautau, ka e ngā te tohu kōrekoreko free ki 25 (OH) VD i runga i te kukū membrane.The o 25 (OH) VD he ine kino hoki fluorescence tohu, me te kukū o 25 (OH) VD i roto i te tauira e taea te kitea i te fluorescence immunoassay anga matou.

Tauhohenga ME MATERIALS tukua

Wae mōkihi 25T :
kāri .Test fakafo'ituitui konumohe whakapūkoro ki te desiccant 25T
.He otinga 25T
otinga .B 1
.Package kōkuhu 1

MATERIALS hiahiatia ENGARI KĀORE HOROARAA
kohinga Tauira ipu, matawā

TAUIRAITI kohinga me te rokiroki
1.The tauira whakamatauria e taea e serum, heparin anticoagulant wē EDTA wē anticoagulant ranei.

2.According ki ngā rautaki paerewa kohikohi tauira. Ka taea te puritia serum wē tauira ranei te paemahana i 2-8 ℃ mō 7days me cryopreservation i raro -15 ° C mō te 6 marama.
3.All tauira faka'ehi'ehi huringa Freeze-koero.

Te anga Hātepe
te tukanga whakamātau o te mea kite i te ā-immunoanalyzer. Ko te tukanga kaiwhakahohe whakamātautau kei rite whai

1.Lay peka tauhohenga me ngā tauira katoa ki te pāmahana rūma.
2.Open te Kawe Immune Analyzer (Wiz-A101), tomo te takiuru kupuhipa pūkete rite ki te tikanga mahi o te mea, ka tomo i te atanga rapunga.
3.Scan te waehere dentification ki te whakaū i te tūemi whakamātautau.
4.Take i te kāri whakamātautau i te putea konumohe.
5.Insert te kāri whakamātautau ki te mokamoka kāri, te matawai i te waehere UT, ka whakatau i te tūemi whakamātautau.
6.Add 30μL serum wē tauira ki te otinga ranei, a pai uru.
Otinga 7.Add 50μL B ki te ranunga i runga, a pai uru.
8 .Waiho i te ranunga mō te 15 meneti.
9.Add 80μL ranunga ki te rapu pai o te kāri.
10.Click te pātene "whakamātautau paerewa", i muri i te 10 meneti, te mea e kitea aunoa i te kāri whakamātautau, ka taea e te reira te pānui i te hua i te mata whakaatu o te mea, me record / tā te hua whakamātautau.
11.Refer ki te ako o Kawe Immune Analyzer (Wiz-A101).

Uara e tika ana
25 (OH) VD whānui noa: 30-100ng / mL

E tūtohutia ana e ia taiwhanga whakapumautia ona ake ngā noa e tohu tona taupori manawanui.

HUA TEST ME Tuhinga
.Ko i runga raraunga ko te wā tohutoro whakapumautia mo nga raraunga rapunga o tenei kete, a ko te whakaaro, e kia whakapumautia ia taiwhanga he wā tohutoro mo te hiranga haumanu hāngai o te taupori i roto i tenei rohe.

.Ko kukū o 25 (OH) VD ko runga ake i te whānuitanga tohutoro, a kia kia excluded.Indeed rerekē nga huringa whaiaroaro ahotea whakautu ranei, kia paheko tātaritanga tohumate haumanu.
E hāngai ana anake ki te whānuitanga tohutoro whakapumautia e tenei tikanga .Ko hua o tenei tikanga, a kihai e tika i rite nei ki ētahi atu tikanga nga hua.
Ka taea e ngā āhuatanga .Ko meinga hoki hapa i roto i ngā hua rapunga, tae atu ki ngā take hangarau, hapa whakahaere me te tahi atu āhuatanga tauira.

Tuhinga me te pūmautanga
.Ko kete ko 18 marama papa-ora i te rā o te whakanao. Rokiroki i te kete kāore i 2-30 ° C. KAUA E MOMOKÓ. Kaua e whakamahi i tua atu i te rā paunga.

.Kaua e whakatuwhera te putea hiri noa e rite ki te mahi i te whakamātautau koe, ka te whakaaro te kotahi-whakamahi whakamātautau te ki kia whakamahia i raro i te taiao e hiahiatia (pāmahana 2-35 ℃, haumākū 40-90%) i roto i 60 mins rite hohoro rite taea.
Whakamahia diluent .Sample te tonu i muri i te whakatuwheratia.

Ngaahi fakatokanga mo e whakatūpatotanga
kia .Ko kete kia hiritia, ka tiaki ki te houku.

.Ko ka tauira pai kia whakamana e te tahi atu tikanga.
Ka tukinotia tauira .Ko rite parakino pea.
.Kaua e whakamahi kaiwhakahohe pau.
.Kaua e Whakawhitiwhiti tauhohenga i roto i kete ki rerekē rota kahore ..
.Kaua e whakamahi anō kāri whakamātautau me tetahi oko tuku.
.Misoperation, Ka taea e te tauira nui iti ranei arahi ki te hua ine.

L ia peehia
.Ko ki tetahi anga matou mahi paturopi kiore, te vai te taea hoki pokanoa i paturopi anti-kiore tangata (HAMA) i roto i te tauira. Ngā tauira i te tūroro e kua riro taka o paturopi monoclonal mō te tātaritanga therapy kia roto HAMA ranei. Taua tauira kia meinga hua kino teka pai teka ranei.

.Ko hua whakamātautau te anake mo te tohutoro haumanu, e kore e mahi i rite anake te pūtake mō te tātaritanga haumanu me te maimoatanga, kia waiho nga tūroro whakahaere haumanu whakaaro whānui ngā ki ona tohu, hītori hauora, atu whakamātautau taiwhanga, maimoatanga whakautu, mātai tahumaero me ētahi atu mōhiohio .
Kei te anake whakamahia .Ko kaiwhakahohe mō whakamātautau serum me te wē. E kore e kia whiwhi reira hua tika, ka whakamahia mō te tahi atu tauira pērā i huware me mimi me etc.

ĀHUATANGA MAHINGA

Linearity 5 ng / mL ki te 120 ng / mL ine whanaunga: -15% ki + 15%.
Linear whakarea ine pānga: (r) ≥0.9900
tika ka te auau whakaora i roto i te 85% - 115%.
Repeatability CV≤15%

(tetahi o nga matū i te interferent whakamatauria pokanoa i roto i te anga matou)
Interferent kukū Interferent
pūtoto 200μg / mL
transferrin 100μg / mL
peroxidase rarihi Horse 2000μg / mL
huaora D3 50mg / mL
huaora D 50mg / mL

R EFERENCES

1.Hansen JH, et al.HAMA te Whakararuraru ki Immunoassays Murine Monoclonal antibody-I [J] .MAOR o Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.The Nature o Heterophilic paturopi me te Tūnga i roto i Immunoassay te Whakararuraru [J] .MAOR o Clin Immunoassay, 1992,15: 108-114.

Key ki tohu whakamahia:

 t11-1 I roto i vitro Tātaritanga Pūrere Medical
 tt-2 Manufacturer
 tt-71 Store i 2-30 ℃
 tt-3 rā paunga
 tt-4 Kaua Kāore Whakamahi anō
 tt-5 WHAKATŪPATO
 tt-6 Consult Tohutohu hoki Whakamahi

Xiamen Wiz Biotech CO, LTD.
Wāhi noho: 3-4 Floor, no.16 Building, Workshop Bio-hauora, 2030 Wengjiao Te Hau-ā-uru Road, Haicang District, 361026, Xiamen, China
Teremera: + 86-592-6808278
Waea: + 86- 592-6808279


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