Diagnostic Kit(Golide wa Colloidal）kwa Calprotectin
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo.Kudalirika kwa zotsatira zoyeserera sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOYENERA KUGWIRITSA NTCHITO
Diagnostic Kit for Calprotectin(cal) ndi colloidal gold immunochromatographic assay for semiquantitative cal kuchokera ku ndowe za anthu, zomwe zimakhala ndi zofunikira zowunikira matenda otupa a m'mimba.Chiyeso ichi ndi chowunikira.Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.Kuyezetsaku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.Pakadali pano, mayesowa amagwiritsidwa ntchito pa IVD, zida zowonjezera sizikufunika.

CHIDULE
Cal ndi heterodimer, yomwe imapangidwa ndi MRP 8 ndi MRP 14. Imapezeka mu neutrophils cytoplasm ndipo imawonetsedwa pamagulu a cell a mononuclear.Cal ndi pachimake gawo mapuloteni, ali bwino khola gawo pafupifupi sabata imodzi mu ndowe za anthu, izo anatsimikiza kukhala kutupa m`mimba chizindikiro.Chidachi ndi mayeso osavuta, owonera semiqualitative omwe amazindikira cal mu ndowe za anthu, amakhala ndi chidwi chodziwikiratu komanso mawonekedwe amphamvu.Mayeso otengera ma antibodies awiri a sandwich reaction mfundo ndi golide immunochromatographic assay technics, amatha kupereka zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRA
Mzerewu uli ndi anti cal coating McAb pagawo loyesa komanso anti-Rabbit IgG antibody pachigawo chowongolera, chomwe chimamangiriridwa ku membrane chromatography pasadakhale.Lable pad amakutidwa ndi golide wa colloidal wolembedwa kuti anti cal McAb ndi golide wa colloidal wotchedwa IgG antibody ya kalulu.Mukayesa zitsanzo zabwino, ma cal mu zitsanzo adapangidwa ndi golide wa colloidal otchedwa anti cal McAb, ndikupanga chitetezo chamthupi, chifukwa amaloledwa kusuntha motsatira mzere woyeserera, cal conjugate complex imagwidwa ndi anti cal coating McAb pa nembanemba ndi mawonekedwe. "anti cal coating McAb-cal-colloidal gold lolembedwa kuti anti cal McAb", gulu loyesera lamitundumitundu lidawonekera pagawo loyesa.Kuchuluka kwamtundu kumalumikizidwa bwino ndi zomwe zili ndi cal.Chitsanzo choyipa sichimapanga gulu loyesera chifukwa chosowa colloidal gold conjugate cal complex.Ziribe kanthu kuti cal ilipo mu zitsanzo kapena ayi, pali mikwingwirima yofiyira yomwe imawonekera pagawo loyang'anira komanso chigawo chowongolera, chomwe chimatengedwa ngati miyezo yamabizinesi amkati.

REAGENTS NDI Zipangizo ZOPEREKA
Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant
.Sample diluents: zosakaniza ndi 20mM pH7.4PBS
.Disette
.Kuyika phukusi

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA

Chidebe chosonkhanitsira zitsanzo, chowerengera nthawi

KUSONKHA ZITSANZO NDI KUSINTHA
Gwiritsani ntchito chidebe choyera chotayirapo kuti mutenge ndowe zatsopano, ndikuyezetsani nthawi yomweyo.Ngati simungathe kuyesedwa nthawi yomweyo, chonde sungani pa 2-8 ° C kwa 12hours kapena chisanu -15 ° C kwa miyezi inayi.

NTCHITO YOSANGALALA
1. Tulutsani ndodo, ikani mu ndowe, kenaka bwezerani ndodoyo, pukuta mwamphamvu ndikugwedezani bwino, bwerezani zomwezo katatu.Kapena kugwiritsa ntchito zitsanzo zomata za 50mg za ndowe, ndikuyika mu chubu cha ndowe chokhala ndi dilution, ndikupukuta mwamphamvu.
2. Gwiritsani ntchito zitsanzo za pipette zotayidwa tengani ndowe zowonda kwambiri za wodwala matenda otsekula m'mimba, kenaka onjezerani madontho atatu (pafupifupi 100uL) ku chubu chopangira ndowe ndikugwedezani bwino, ikani pambali.
3.Tulutsani khadi loyesera kuchokera m'thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikulemba chizindikiro.
4.Chotsani kapu kuchokera ku chubu lachitsanzo ndikutaya madontho awiri oyambirira osungunuka, onjezerani madontho a 3 (pafupifupi 100uL) palibe bubble diluted chitsanzo verticaly ndi pang'onopang'ono mu chitsanzo chabwino cha khadi ndi dispette anapereka, kuyamba nthawi.
5.Zotsatira ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo ndizosavomerezeka pakatha mphindi 15.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA

KUSINTHA NDI KUKHALA
Zidazi ndi miyezi 24 ya alumali kuyambira tsiku lopangidwa.Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C.Osatsegula thumba lomata mpaka mutakonzeka kuyesa.
CHENJEZO NDI CHENJEZO
1.Chidacho chiyenera kusindikizidwa ndikutetezedwa ku chinyezi¹.
2.Musagwiritse ntchito chitsanzo chomwe chimayikidwa motalika kwambiri kapena mobwerezabwereza kuzizira ndi kusungunuka kuti muyese
3.Fecal zitsanzo ndi mopambanitsa kapena makulidwe akhoza kuchepetsedwa zitsanzo zoipa mayeso khadi, chonde centrifuge chitsanzo kuchepetsedwa ndi kutenga supernatant kuyezetsa.
4.Misoperation, zambiri kapena zochepa zitsanzo zingayambitse zotsatira zolakwika.

MALIRE
1.Zotsatira zoyezetsazi ndizongofotokozera zachipatala, siziyenera kukhala chifukwa chokhacho chodziwira matenda ndi chithandizo chamankhwala, kasamalidwe ka odwala ayenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, mayeso ena a labotale, kuyankha kwamankhwala, miliri ndi zina. zambiri².
2.Reagent iyi imagwiritsidwa ntchito poyesa ndowe.Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.