MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH)VD pamalo oyesera ndi antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya fluorescence mark anti 25-(OH)VD ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo, 25-(OH)VD mu chitsanzo imaphatikizana ndi antibody ya fluorescence mark antibody ya 25-(OH)VD, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zadutsa pamalo oyesera, chizindikiro cha fluorescent chaulere chidzaphatikizidwa ndi 25-(OH)VD pa nembanemba. Kuchuluka kwa 25-(OH)VD ndi mgwirizano woipa wa chizindikiro cha fluorescence, ndipo kuchuluka kwa 25-(OH)VD mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

Njira Yoyesera:

Chonde werengani buku la malangizo ogwiritsira ntchito chipangizocho ndi phukusi la phukusi musanayese.

1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tulutsani khadi loyesera m'thumba la foil.
3. Onjezani 0μL seramu/plasma/magazi athunthu/chala mu yankho, ndipo sakanizani bwino.
4. Werengani zotsatira za mayeso mkati mwa mphindi 15.