Matenda zida kwa 25 hydroxy Vitamini D (kuwala immunochromatographic zofufuza)

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Matenda zida kwa 25 hydroxy Vitamini D (kuwala immunochromatographic zofufuza) Pakuti m'galasi ntchito matenda yekha Chonde werengani phukusili amaika mosamala asanayambe ntchito ndi mosamalitsa malangizo. Kudalirika kwa zotsatira zofufuza sangathe amayenda ngati pali zolakwika chilichonse malangizo izi Ikani phukusi. Angazigwiritse NTCHITO matenda zida kwa 25 hydroxy Vitamini D (kuwala immunochromatographic zofufuza) ndi kuwala immunochromatographic zofufuza kwa anthu d kachulukidwe ...


mankhwala Mwatsatanetsatane

Matenda zida kwa 25 hydroxy Vitamini D (kuwala immunochromatographic zofufuza)
Pakuti m'galasi ntchito matenda yekha

Chonde werengani phukusili amaika mosamala asanayambe ntchito ndi mosamalitsa malangizo. Kudalirika kwa zotsatira zofufuza sangathe amayenda ngati pali zolakwika chilichonse malangizo izi Ikani phukusi.

Angazigwiritse NTCHITO
matenda zida kwa 25 hydroxy Vitamini D (kuwala immunochromatographic zofufuza) ndi kuwala immunochromatographic zofufuza chifukwa kudziwika kachulukidwe 25-hydroxy Vitamini D (25- (o) VD) mu seramu wa anthu kapena plasma, amene zimagwiritsa ntchito kupenda amaudindo vitamini D.It ndi wothandiza matenda reagent.All zabwino chitsanzo ayenera anatsimikizira mwa njira zina. Kuyeza kumeneku cholinga ntchito chisamaliro chaumoyo akatswiri yekha.

SUMMARY
Vitamini D ndi vitamini ndipo alinso timadzi steroid, makamaka kuphatikizapo VD2 ndi VD3, amene struction ndi ofanana kwambiri. Vitamini D3 ndi D2 mtima kwa 25 hydroxyl vitamini D (kuphatikizapo 25 dihydroxyl vitamini D3 ndi D2). 25- (o) VD mu thupi la munthu, khola struction, mkulu ndende. 25- (o) VD amasonyeza chiwerengero cha vitamini D, ndi luso kutembenuka vitamini D, kotero 25- (o) VD akuti ndi chizindikiro yabwino imene ingatithandize kuzindikira mlingo wa vitamini D.The matenda zida zachokera immunochromatography ndi angapereke chifukwa mkati mphindi 15.

MFUNDO ZA ndondomeko
The Kakhungu chipangizo mayeso TACHIMATA ndi conjugate wa BSA ndi 25- (o) VD pa dera mayeso ndi mbuzi odana kalulu IgG oteteza pa dera ulamuliro. PAD Marker ndi TACHIMATA ndi kuwala chizindikiro odana 25- (o) VD oteteza ndi kalulu IgG pasadakhale. Pamene kudziyesa chitsanzo, 25- (o) VD mu chitsanzo zimagwirizizirana ndi kuwala amalembedwa odana 25- (o) VD oteteza ndi kupanga osakaniza m'thupi. Mu zochita za immunochromatography ndi kulandira zovuta motsogozedwa pepala absorbent, pamene zovuta anadutsa m'dera mayeso, The ufulu fulorosenti chikhomo adzakhala pamodzi 25- (o) VD pa membrane.The ndende ya 25- (o) VD ndi zoipa malumikizanidwe kwa kuwala chizindikiro, ndipo anthu ambiri 25- (o) VD mu nyemba angathe kuonedwa ndi kuwala immunoassay zofufuza.

Reagents NDI zipangizo

Zigawo 25T phukusi :
.Test khadi payekha zojambulazo pouched ndi desiccant 25T
.A njira 25T
.B njira 1
.Package amaika 1

Zipangizo Amafuna KOMA sinabweretse
Zitsanzo zosonkhanitsira chidebe, powerengetsera

SAMPLE Collection ndi kusunga
1. zitsanzo anayesedwa kungakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.

2.According kuti njira muyezo kusonkhanitsa nyemba. Seramu kapena plasma nyemba akhoza kusungidwa m'firiji pa 2-8 ℃ kwa 7days ndi cryopreservation m'munsimu -15 ° C kwa miyezi 6.
3.All chitsanzo Pewani amaundana-m'firiji pamtunda m'zinthu.

Zofufuza ndondomeko
The mayeso ndondomeko ya chida cha kuona immunoanalyzer Buku. Ndondomeko reagent chiyeso motere

1.Lay pambali reagents onse ndi zitsanzo kwa firiji.
2.Open ndi zam'manja Timakumana chowunikira (WIZ-A101), lowa nkhani achinsinsi malowedwe mogwirizana ndi njira ntchito chida, ndi kulowa mawonekedwe kudziwika.
3.Scan ndi dentification code ku atsimikizire item mayeso.
4.Take kunja khadi mayeso ku thumba zojambulazo.
5.Insert mayeso khadi mu khadi kagawo, aone QR code, ndi kudziwa item mayeso.
6.Add 30μL seramu kapena plasma chitsanzo njira A, ndi kusakaniza bwino.
7.Add 50μL B njira kusakaniza pamwamba, ndi kusakaniza bwino.
8 .Siyani osakaniza kwa mphindi 15.
9.Add 80μL osakaniza ndi kuyesa bwino a khadi.
10.Click "zovomerezedwa ndi kuyesedwa" batani, pambuyo pa maminitsi 10, chida cha adzakhala basi kudziwa khadi mayeso, tingathe kuwerenga zotsatira za nsalu yotchinga kuwonetsera chida, ndi mbiri / kusindikiza zotsatira mayeso.
11.Refer malangizo a zam'manja Timakumana chowunikira (WIZ-A101).

AKHRISTU NDI MAKHALIDWE ABWINO
25- (o) VD yachibadwa osiyanasiyana: 30-100ng / mL

Ndi bwino kuti aliyense zasayansi kukhazikitsa yachibadwa yake osiyanasiyana woimira anthu ake mtima.

Mayeso zotsatira ndi kutanthauzira
.The pamwamba deta ndi Buku imeneyi unakhazikitsidwa kuti deta kudziwika zida izi, ndi akuti aliyense zasayansi ayenera kukhazikitsa Buku imeneyi kwa zogwirizana ndi tanthauzo matenda alionse m'dera lino.

.The ndende ya 25- (o) VD ndi apamwamba kuposa osiyanasiyana Buku, ndi kusintha thupi kapena ayankhe nkhawa ayenera excluded.Indeed nthenda, ayenera kuphatikiza matenda chizindikiro matenda.
.The zotsatira za njira izi zimagwira ntchito yokhayo kutchulidwa osiyanasiyana anakhazikitsa njira imeneyi, ndipo zotsatira sali ofanana ndi njira zina.
Zinthu .Other zina chifukwa zolakwa zotsatira kudziwika, kuphatikizapo zifukwa zina, zolakwa ntchito ndi zinthu zina nyemba.

Yosungirako ndi kukhazikika
.The zida ndi miyezi 18 alumali moyo kuchokera tsiku kupanga. Kusunga zida za makuponi pa 2-30 ° C. KODI amaundana. Musagwiritse ntchito kupitirira tsiku lothera ndi.

.Do sanatsegule chinthucho munasindikizidwa nawo kufikira ndinu wokonzeka kuchita mayeso, ndipo limodzi ntchito mayeso akuti kugwiritsidwa ntchito pa chilengedwe chofunika (kutentha 2-35 ℃, chinyezi 40-90%) mwa 60 mphindi mwamsanga zotheka.
.Sample diluent ntchito yomweyo pambuyo anatsegula.

MACHENJEZO ndi njira zopewera
.The zida ayenera losindikizidwa ndi kutetezedwa ku chinyezi.

.All toyesa zabwino tidzakwatulidwa linatsimikizidwa ndi njira zina.
Toyesa .All adzakhala kuchitiridwa ngati zoipa zopezeka angathe.
.DO ntchito anatsirizika reagent.
.DO OSATI kutonthozana reagents mwa zida ndi zambiri zosiyana palibe ..
.DO OSATI zingagwiritsidwenso ntchito makadi mayeso ndi Chalk chilichonse chopanda ntchito.
.Misoperation, amaika kapena pang'ono chitsanzo kungachititse kuti zotsatira zolakwika.

L onyenga
.As ndi zofufuza ntchito chitetezo chilichonse mbewa, n'zotheka kutichitikira kusokonezedwa ndi chitetezo anthu odana ndi mbewa (mzinda) ku chitsanzo cha. Toyesa kwa odwala amene alandira kukonzekera za chitetezo monoclonal kwa matenda kapena mankhwala anyamula mzinda. Toyesa amenewa zikhoza onyenga zotsatira zabwino kapena zonama negative.

.This chifukwa mayeso kokha kuti adzigwiritsidwa ntchito yachipatala, sayenera kukhala ngati maziko okha matenda matenda ndi mankhwala, odwala kasamalidwe matenda ayenera kukhala wopambana kuganizira pamodzi ndi zizindikiro zake, mbiri zachipatala, zasayansi Kupenda zina, chithandizo Poyankha, EPIDEMIOLOGY ndi zina .
Reagent .This kokha ntchito seramu ndi madzi a m'magazi mayesero. Sangathe kupeza zotsatira zolondola ntchito zitsanzo zina monga malovu ndi mkodzo ndi etc.

Magwiridwe makhalidwe

Linearity 5 ng / mL kwa 120 ng / mL wachibale kupatuka: -15% kwa + 15%.
Liniya malumikizanidwe koyefishienti: (R) ≥0.9900
Zowona Mlingo kuchira adzakhala mkati 85% - 115%.
Repeatability CV≤15%
Kulunjika
(Palibe zinthu pa interferent mayesedwe zinasokoneza mu zofufuza za)
Interferent Interferent ndende
hemoglobin 200μg / mL
transferrin 100μg / mL
Kavalo radish peroxidase 2000μg / mL
vitamini D3 50mg / mL
vitamini D 50mg / mL

R EFERENCES

1.Hansen JH, neri al.HAMA kusokonezedwa ndi Murine Monoclonal oteteza-yachokera Immunoassays [J] .J wa Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.The Nature wa Heterophilic chitetezo ndi Udindo mu Immunoassay kusokonezedwa [J] .J wa Clin Immunoassay, 1992,15: 108-114.

Kiyi zizindikiro ntchito:

 t11-1 Mu m'galasi matenda Medical Chipangizo
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 TT 71 Store pa 2-30 ℃
 TT-3 Tsiku lothera ntchito
 TT-4 Musakhale zingagwiritsidwenso
 TT-5 CHENJEZO
 TT-6 Funani Malangizo ntchito

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