Zida Zodziwira Matenda a Matenda aCalprotectin(Kuyesa kwa Fluorescence Immunochromatographic)
Kugwiritsa ntchito poyezetsa matenda m'thupi kokha
Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA
Zida Zodziwira Matenda a Matenda aCalprotectin(Cal) ndi yoyenera kudziwa kuchuluka kwa ndowe za anthu. Cal pogwiritsa ntchito njira yowunikira ya fluorescence immunochromatographic, yomwe ili ndi phindu lofunikira pozindikira matenda otupa m'matumbo. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE
Cal ndi heterodimer, yomwe imapangidwa ndi MRP 8 ndi MRP 14.^[1]Imapezeka mu cytoplasm ya neutrophils ndipo imapezeka pa nembanemba ya maselo a mononuclear. Cal ndi mapuloteni a acute phase, ali ndi gawo lokhazikika pafupifupi sabata imodzi m'ndowe za anthu, ndipo yapezeka kuti ndi chizindikiro cha matenda otupa m'matumbo.^[2-3]. Chida ichi ndi chosavuta, chowoneka bwino chomwe chimazindikira Cal m'ndowe za anthu, chimakhala ndi kuzindikira kwakukulu komanso kutsimikizika kwamphamvu. Chiyesochi chimachokera ku mfundo yayikulu ya double antibodies sandwich reaction principle ndi fluorescence immunochromatographic assay analysis technics, zomwe zingapereke zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRAYI
Chingwecho chili ndi anti-Cal coating antibody pamalo oyesera, omwe amamangiriridwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi anti-Cal antibody yolembedwa kuti fluorescence. Poyesa chitsanzo chabwino, Cal mu chitsanzocho imatha kusakanikirana ndi anti-Cal antibody yolembedwa kuti fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pamene chisakanizocho chikuloledwa kusuntha motsatira mzere woyesera, Cal conjugate complex imagwidwa ndi anti-Cal coating antibody pa membrane ndikupanga complex. Mphamvu ya fluorescence imagwirizanitsidwa bwino ndi kuchuluka kwa Cal. Cal mu chitsanzocho imatha kuzindikirika ndi fluorescence immunoassay analyzer.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA

Zigawo za phukusi la 25T：
Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant 25T
Zitsanzo zoyeretsera 25T
Phukusi lowonjezera 1

Zipangizo zofunika koma siziperekedwa
Chidebe chosonkhanitsira zitsanzo, nthawi

Zitsanzo Zosonkhanitsira ndi Kusunga
1. Gwiritsani ntchito chidebe choyera chomwe chingagwiritsidwe ntchito nthawi yomweyo kuti mutenge ndowe zatsopano, ndipo muyesedwe nthawi yomweyo. Ngati simungathe kuziyesa nthawi yomweyo, chonde sungani pa 2-8°C kwa masiku atatu kapena pansi pa -15°C kwa miyezi 6.
2. Tulutsani ndodo yoyesera, yoyikidwa mu chitsanzo cha ndowe, bwerezani zomwe mwachita katatu, tengani magawo osiyanasiyana a chitsanzo cha ndowe nthawi iliyonse, kenako ikani ndodo yoyesera kumbuyo, gwirani mwamphamvu ndikugwedeza bwino, Kapena pogwiritsa ntchito ndodo yoyesera yomwe mwasankha pafupifupi 50mg ya ndowe, ndikuyika mu chubu choyesera cha ndowe chokhala ndi kusungunuka kwa chitsanzo, ndikuyika mwamphamvu.
3. Gwiritsani ntchito njira yochotsera ndowe m'madzi otuluka m'thupi. Tengani ndowe kuchokera kwa wodwala matenda otsegula m'mimba, kenako onjezerani madontho atatu (pafupifupi 100µL) mu chubu chochotsera ndowe ndikugwedeza bwino.

Zolemba:
1. Pewani kuzizira ndi kusungunula zinthu.
2. Sungunulani zitsanzozo kutentha kwa chipinda musanagwiritse ntchito.

NJIRA YOYESERA

Chonde werengani buku la malangizo ogwiritsira ntchito chipangizocho ndi phukusi la phukusi musanayese.
1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi olowera mu akaunti malinga ndi njira yogwirira ntchito ya chidacho, ndikulowetsa mawonekedwe ozindikira.
3.Scan code yodziwitsa kuti mutsimikizire chinthu choyesera.
4. Tulutsani khadi loyesera m'thumba la foil.
5. Ikani khadi loyesera mu malo oikamo khadi, sikani QR code, ndikuzindikira chinthu choyesera.
6. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezerani madontho atatu (pafupifupi 100uL) opanda thovu lochepetsedwa molunjika ndipo pang'onopang'ono mu chitsime cha chitsanzo cha khadi chomwe chili ndi dispette yoperekedwa.
7. Dinani batani la "standard test", patatha mphindi 15, chidacho chidzazindikira khadi loyesera lokha, chimatha kuwerenga zotsatira kuchokera pazenera lowonetsera chidacho, ndikulemba/kusindikiza zotsatira za mayeso.
8. Onani malangizo a Portable Immune Analyzer (WIZ-A101).

MFUNDO ZOYEMBEKEZEDWA
Ma calories <60μg/g
Ndikofunikira kuti labotale iliyonse ikhazikitse kuchuluka kwake komwe kumayimira odwala ake.

Zotsatira za Mayeso ndi Kutanthauzira
1. Cal yomwe ili mu chitsanzo ndi yoposa 60μg/g, ndipo iyenera kuletsa kusintha kwa momwe thupi limagwirira ntchito. Zotsatira zake sizodabwitsa ndipo ziyenera kupezeka ndi zizindikiro zachipatala.
2. Zotsatira za njira iyi zimagwira ntchito kokha pa ma reference ranges omwe akhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa mwachindunji ndi njira zina.
3. Zinthu zina zingayambitsenso zolakwika pakupeza zotsatira, kuphatikizapo zifukwa zaukadaulo, zolakwika pakugwira ntchito ndi zinthu zina zachitsanzo.

KUSUNGA NDI KUKHALA BWINO
1. Chidachi chimakhala ndi moyo wa miyezi 18 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. MUSAZIYIKE MU MAFURI. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2. Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso, ndipo mayeso ogwiritsidwa ntchito kamodzi akulangizidwa kuti agwiritsidwe ntchito pamalo ofunikira (kutentha 2-35℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere.
3. Chitsanzo cha diluent chimagwiritsidwa ntchito nthawi yomweyo chikatsegulidwa.

MACHENJEZO NDI ZOCHENJEZERA
.Zida ziyenera kutsekedwa ndi kutetezedwa ku chinyezi.
Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.
Zitsanzo zonse ziyenera kuonedwa ngati zowononga chilengedwe.
MUSAGWIRITSE NTCHITO reagent yotha ntchito.
MUSASINTHIRE ma reagent pakati pa zida zomwe zili ndi nambala yosiyana.
MUSAGWIRITSE NTCHITO makhadi oyesera ndi zinthu zina zilizonse zogwiritsidwa ntchito ngati zinyalala.
Kugwiritsa ntchito molakwika, kuchuluka kwa zitsanzo kapena zochepa kungayambitse kusintha kwa zotsatira.

MALIRE
Monga momwe zimakhalira ndi mayeso aliwonse omwe amagwiritsa ntchito ma antibodies a mbewa, pali kuthekera kwa kusokonezedwa ndi ma antibodies a anti-mbewa a anthu (HAMA) mu chitsanzocho. Zitsanzo kuchokera kwa odwala omwe adalandira ma antibodies a monoclonal kuti apeze matenda kapena chithandizo zitha kukhala ndi HAMA. Zitsanzo zotere zingayambitse zotsatira zabodza kapena zabodza.
Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, kuyang'anira odwala kuyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwina kwa labotale, yankho la chithandizo, matenda a epidemiology ndi zina zambiri.
Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa ndowe zokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.

MAKHALIDWE A NTCHITO

MAWU OTHANDIZA
1.Li,G.&Y.L.Li.Ubale pakati pa calcium ndi matenda azachipatala[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al. Kafukufuku wachipatala wa calamine ndi lactoferrin mwa odwala omwe ali ndi ulcerative colitis [J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al. Kafukufuku wokhudza ubale pakati pa calamine wa m'chimbudzi ndi matenda otupa m'matumbo [J]. Science Technology and Engineering,2010-03,10(8)

Chinsinsi cha zizindikiro zomwe zagwiritsidwa ntchito:

	Chipangizo Chachipatala Chodziwitsira Matenda mu Vitro
	Wopanga
	Sungani pamalo otentha 2-30℃
	Tsiku lothera ntchito
	Musagwiritsenso ntchito
	CHENJEZO
	Onani Malangizo Ogwiritsira Ntchito

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