Diagnostic Kit kwaCalprotectin(Fluorescence Immunochromatographic Assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo.Kudalirika kwa zotsatira zoyeserera sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOYENERA KUGWIRITSA NTCHITO
Diagnostic Kit kwaCalprotectin(Cal) ndi yoyenera kutsimikizira kuchuluka kwa ndowe za anthu Cal ndi fluorescence immunochromatographic assay, yomwe ili ndi zofunikira zowunikira matenda otupa m'matumbo. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.Kuyezetsaku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE
Cal ndi heterodimer, yomwe imapangidwa ndi MRP 8 ndi MRP 14^[1].Imapezeka mu neutrophils cytoplasm ndipo imawonetsedwa pa nembanemba yama cell a mononuclear.Cal ndi mapuloteni owopsa, ali ndi gawo lokhazikika pafupifupi sabata imodzi m'ndowe za anthu, atsimikiza kukhala chizindikiro cha matenda a m'mimba.^[2-3].Chidachi ndi mayeso osavuta, owoneka bwino omwe amazindikira Cal mu ndowe za anthu, amakhala ndi chidwi chodziwikiratu komanso mawonekedwe amphamvu.Mayeso otengera kutsimikizika kwakukulu kwa ma antibodies awiri a sandwich reaction mfundo ndi fluorescence immunochromatographic assay technics, omwe angapereke zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRA
Mzerewu uli ndi anti-Cal coating antibody pamalo oyesera, omwe amamangiriridwa ku membrane chromatography pasadakhale.Lable pad imakutidwa ndi fluorescence yotchedwa anti-Cal antibody pasadakhale.Mukayesa zitsanzo zabwino, Cal mu zitsanzo zimatha kusakanizidwa ndi fluorescence yotchedwa anti-Cal antibody, ndikupanga osakaniza a chitetezo chamthupi.Pamene kusakaniza kumaloledwa kusuntha motsatira mzere woyesera, Cal conjugate complex imatengedwa ndi anti-Cal coating antibody pa nembanemba ndikupanga zovuta.Kuchuluka kwa fluorescence kumagwirizana bwino ndi zomwe Cal zili.Cal mu zitsanzo amatha kuzindikirika ndi fluorescence immunoassay analyzer.

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:
Khadi yoyesera payokha zojambulazo zopakidwa ndi desiccant 25T
Zitsanzo za diluent 25T
Phukusi 1

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer

KUSONKHA ZITSANZO NDI KUSINTHA
1.Gwiritsani ntchito chidebe choyera chotayidwa kuti mutenge ndowe zatsopano, ndikuyesedwa nthawi yomweyo.Ngati simungathe kuyesedwa nthawi yomweyo, chonde sungani pa 2-8 ° C kwa masiku atatu kapena pansi -15 ° C kwa miyezi 6.
2. Tulutsani ndodo, ikani mu ndowe, bwerezani zomwezo katatu, tengani magawo osiyanasiyana a ndowe nthawi iliyonse, kenaka bwezerani ndodo, pukutani bwino ndikugwedezani bwino, Kapena kugwiritsa ntchito ndodo yomwe mwatola. pafupifupi 50mg ndowe chitsanzo, ndi kuika mu ndowe chitsanzo chubu chokhala ndi dilution chitsanzo, ndi wononga mwamphamvu.
3. Gwiritsani ntchito zitsanzo za pipette zotayidwa tengani ndowe za wodwala wotsekula m'mimba, kenaka onjezerani madontho atatu (pafupifupi 100µL) ku chubu chopangira ndowe ndikugwedezani bwino.

Ndemanga:
1. Pewani kuzizira kozizira.
2.Thaw zitsanzo kutentha kutentha musanagwiritse ntchito.

NTCHITO YOSANGALALA

Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese.
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Chotsani kapu kuchokera ku chubu lachitsanzo ndikutaya madontho awiri oyambirira osungunuka, onjezerani madontho a 3 (pafupifupi 100uL) palibe kuwira kuchepetsedwa chitsanzo verticaly ndi pang'onopang'ono mu chitsanzo bwino khadi ndi dispette anapereka.
7.Dinani batani la "standard test", pakatha mphindi 15, chidacho chidzangozindikira khadi yoyesera, imatha kuwerenga zotsatira kuchokera pachiwonetsero chowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
8. Onani malangizo a Portable Immune Analyzer(WIZ-A101).

MFUNDO ZOYENERA
Kalori <60μg/g
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA
1. Cal mu chitsanzo ndi yoposa 60μg / g, ndipo iyenera kuletsa kusintha kwa chikhalidwe cha thupi.Zotsatira zake zimakhala zachilendo ndipo ziyenera kuzindikiridwa ndi zizindikiro zachipatala.
2.Zotsatira za njirayi zimangogwiritsidwa ntchito pazigawo zofotokozera zomwe zakhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa kwachindunji ndi njira zina.
3.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zina zachitsanzo.

KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga.Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C.OSATI MASIMA.Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kukuyenera kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga. momwe zingathere.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zatha.
.OSATANA kusinthanitsa ma reagents pakati pa zida zomwe zili ndi magawo osiyanasiyana No.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.

MALIRE
.Mofanana ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo.Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA.Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Chitsulochi chimangogwiritsidwa ntchito poyeza ndowe.Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.

ZINTHU ZOCHITIKA

MALONJE
1.Li,G.&Y.L.Li.Ubale pakati pa calcium ndi matenda achipatala[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al.The Clinical Study of calamine and lactoferrin in patients with ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang, ZH, Guo, HB, et al.Kafukufuku wokhudza ubale pakati pa fecal calamine ndi matenda otupa m'matumbo[J].Sayansi Yaukadaulo ndi Umisiri, 2010-03,10(8)

Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito

Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279