Tf imapezeka makamaka mu plasma, kuchuluka kwapakati ndi pafupifupi 1.20 ~ 3.25g/L. Mu ndowe za anthu athanzi, pafupifupi palibe. Pamene magazi akutuluka m'mimba, Tf mu seramu imalowa m'mimba ndikutuluka ndi ndowe, imakhala yochuluka m'ndowe za odwala omwe akutuluka magazi m'mimba. Chifukwa chake, Tf ya ndowe imagwira ntchito yofunika komanso yofunika kwambiri pozindikira magazi akutuluka m'mimba. Kitiyi ndi mayeso osavuta, owoneka bwino omwe amazindikira Tf mu ndowe za anthu, ali ndi kukhudzidwa kwakukulu kozindikira komanso kutsimikizika kwamphamvu. Mayesowa amachokera ku mfundo ya sandwich reaction ya specificit double antibodies ndi golide immunochromatographic assay analysis technics, amatha kupereka zotsatira mkati mwa mphindi 15.