Zida Zodziwira Matenda(a)Golide wa Colloidal）za Calprotectin
Kugwiritsa ntchito poyezetsa matenda m'thupi kokha
Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA
Chida Chodziwira Matenda cha Calprotectin (cal) ndi njira yoyesera ya colloidal gold immunochromatographic yodziwira kuchuluka kwa cal kuchokera m'ndowe za anthu, zomwe zimakhala ndi phindu lofunikira pozindikira matenda otupa m'matumbo. Mayesowa ndi njira yowunikira. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha. Pakadali pano, mayesowa amagwiritsidwa ntchito pa IVD, zida zina sizikufunika.

CHIDULE
Cal ndi heterodimer, yomwe imapangidwa ndi MRP 8 ndi MRP 14. Imapezeka mu cytoplasm ya neutrophils ndipo imaonekera pa nembanemba ya maselo a mononuclear. Cal ndi mapuloteni a acute phase, imakhala ndi gawo lokhazikika bwino pafupifupi sabata imodzi mu ndowe za anthu, imawonedwa ngati chizindikiro cha matenda otupa m'matumbo. Kitiyi ndi mayeso osavuta, owoneka bwino omwe amazindikira cal mu ndowe za anthu, ali ndi kukhudzidwa kwakukulu kozindikira komanso kutsimikizika kwamphamvu. Mayesowa amachokera ku mfundo ya sandwich reaction ya sandwich ya high specificit double antibodies ndi golide immunochromatographic assay analysis technics, amatha kupereka zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRAYI
Chingwecho chili ndi anti-cal coating McAb pamalo oyesera ndi anti-rabbit IgG antibody ya mbuzi pamalo olamulira, yomwe imalumikizidwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi colloidal gold yolembedwa anti-cal McAb ndi colloidal gold yolembedwa anti-IgG antibody ya kalulu pasadakhale. Poyesa chitsanzo chabwino, cal mu chitsanzocho imaphatikizidwa ndi colloidal gold yolembedwa anti-cal McAb, ndipo imapanga immune complex, chifukwa imaloledwa kusuntha motsatira mzere woyesera, cal conjugate complex imagwidwa ndi anti-cal coating McAb pa nembanemba ndikupanga "anti-cal coating McAb-cal-colloidal gold yolembedwa anti-cal McAb", gulu loyesera lamitundu limawonekera pamalo oyesera. Mphamvu ya utoto imagwirizana bwino ndi kuchuluka kwa cal. Chitsanzo choyipa sichipanga gulu loyesera chifukwa chosakhalapo colloidal gold conjugate cal complex. Kaya cal ilipo mu chitsanzo kapena ayi, pali mzere wofiira womwe umawonekera pachigawo chofotokozera ndi dera lowongolera khalidwe, lomwe limawonedwa ngati miyezo yamkati yamakampani abwino.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA
Zigawo za phukusi la 25T：
Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant
Zitsanzo za zosungunulira: zosakaniza zake ndi 20mM pH7.4PBS
.Dispette
.Choyika phukusi

Zipangizo zofunika koma siziperekedwa

Chidebe chosonkhanitsira zitsanzo, chowerengera nthawi

Zitsanzo Zosonkhanitsira ndi Kusunga
Gwiritsani ntchito chidebe choyera chomwe chingagwiritsidwe ntchito nthawi yomweyo kuti mutenge ndowe zatsopano, ndipo muyesedwe nthawi yomweyo. Ngati simungathe kuziyesa nthawi yomweyo, chonde sungani pa 2-8°C kwa maola 12 kapena pansi pa -15°C kwa miyezi 4.

NJIRA YOYESERA
1. Tulutsani ndodo yoyesera, yoyikidwa mu chitsanzo cha ndowe, kenako ikani ndodo yoyesera kumbuyo, ikani mwamphamvu ndikugwedeza bwino, bwerezani zomwezo katatu. Kapena pogwiritsa ntchito ndodo yoyesera yomwe mwasankha pafupifupi 50mg ya ndowe, ndikuyika mu chubu choyesera ndowe chokhala ndi kusungunuka kwa chitsanzo, ndikuyiyika mwamphamvu.
2. Gwiritsani ntchito njira yochotsera ndowe zopyapyala. Tengani chitsanzo cha ndowe zopyapyala kuchokera kwa wodwala matenda otsegula m'mimba, kenako onjezerani madontho atatu (pafupifupi 100uL) mu chubu chochotsera ndowe ndikugwedeza bwino, muyike pambali.
3. Tulutsani khadi loyesera kuchokera mu thumba la foil, liyikeni patebulo lolingana ndikuliyika chizindikiro.
4. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezerani madontho atatu (pafupifupi 100uL) osasungunuka ndi thovu molunjika kenako pang'onopang'ono mu chitsime cha chitsanzo cha khadi ndi dispette yoperekedwa, yambani nthawi.
5. Zotsatira zake ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo sizigwira ntchito patatha mphindi 15.

Zotsatira za Mayeso ndi Kutanthauzira

KUSUNGA NDI KUKHALA BWINO
Chidachi chimakhala ndi moyo wa miyezi 24 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso.
MACHENJEZO NDI ZOCHENJEZERA
1. Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi¹.
2. Musagwiritse ntchito chitsanzo chomwe chimayikidwa nthawi yayitali kapena kuzizira mobwerezabwereza ndikusungunuka kuti muyesere
3. Zitsanzo za ndowe ndi zochulukirapo kapena makulidwe angapangitse zitsanzo zochepetsedwa kukhala khadi loyesera loyipa, chonde perekani chitsanzo chochepetsedwacho pakati ndikutenga supernatant kuti akayesedwe.
4. Kugwiritsa ntchito molakwika, chitsanzo chochuluka kapena chochepa kungayambitse zolakwika.

MALIRE
1. Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, chisamaliro cha odwala chiyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake, mbiri yachipatala, mayeso ena a labotale, yankho la chithandizo, matenda a epidemiology ndi zina zambiri.².
2. Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa ndowe zokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.