MFUNDO

Chipolopolo cha chipangizo choyesera chimakutidwa ndi antibody ya FOB pamalo oyesera ndi antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya FOB yolembedwa fluorescence ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo chabwino, antigen ya FOB mu chitsanzo imaphatikizana ndi antibody ya FOB yolembedwa fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, complex imayenda molunjika ku pepala loyamwa. Complex ikadutsa pamalo oyesera, imaphatikizana ndi antibody ya FOB, imapanga complex yatsopano. Ngati ili ndi negative, chitsanzocho chilibe hemoglobin ya munthu kapena zomwe zili mkati mwake ndizochepa, immune complex zokwanira sizingapangidwe. Sipadzakhala mzere wofiira pamalo ozindikira (T). Mzere wofiira ndiye muyezo womwe umawonekera pamalo owongolera khalidwe (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera:

Chonde werengani zomwe zili mu phukusi musanayese.

1. Tulutsani khadi loyesera kuchokera mu thumba la foil, liyikeni patebulo lolingana ndikuliyika chizindikiro.

2. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezerani madontho atatu (pafupifupi 100uL) opanda thovu lochepetsedwa molunjika komanso pang'onopang'ono mu chitsime cha chitsanzo cha khadi chomwe chili ndi dispette yoperekedwa. Kenako yambani nthawi.

3. Zotsatira zake ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo sizigwira ntchito patatha mphindi 15.