Icyo dukora cyose gifitanye isano n'amahame yacu. "Mbere na mbere, gira icyizere cya mbere, wibanda ku gupfunyika amafunguro no kurengera ibidukikije ku giciro cyo kugurisha cya 2019 mu Bushinwa.Ikizamini cyo gutwita hagati ya 6.0mmAmezi 24 yo kumara igihe kirekire, Dufite ubumenyi mu bicuruzwa by’umwuga n’uburambe buhagije mu gukora. Muri rusange twibwira ko intsinzi yawe ari ubucuruzi bwacu!
Icyo dukora cyose gifitanye isano n'amahame yacu. “Mbere na mbere, gira icyizere cya mbere, wibanda ku gupfunyika amafunguro no kurengera ibidukikije.Igipimo cyo gutwita mu Bushinwa cyagaragaje ubushobozi bwo kugaragaza ibimenyetso by'ubuhanga mu gupima inda ku kigero cya Miu 25, Ikizamini cyo gutwita hagati ya 6.0mmIbikoresho byacu bigezweho, imicungire myiza, ubushakashatsi n'ubushobozi bwo guteza imbere bituma igiciro cyacu kigabanuka. Igiciro dutanga gishobora kuba kitari gito cyane, ariko turahamya ko kizahangana rwose! Murakaza neza kuduhamagara vuba kugira ngo mugire umubano mwiza mu bucuruzi no kugira ngo twese tugire icyo tugeraho!
Agakoresho ko gusuzuma indwara kageneweHormone ya Luteinizingikizamini cya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma imisemburo ya Luteinizing (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima ingano ya Luteinizing Hormone (LH) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma imikorere y'imisemburo ya pituitary. Icyitegererezo cyose cyagaragaye kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE

Umusemburo wa Luteinizing (LH) ni glycoprotein ifite uburemere bwa molekile bugera ku 30.000 Dalton, ikorwa na pituitary y'imbere. Igipimo cya LH gifitanye isano rya hafi no gusohora intanga mu dusabo tw'intanga, kandi igipimo cya LH cyitezwe kuba amasaha 24 kugeza 36 nyuma yo gusohora intanga. Kubwibyo, igipimo cya LH gishobora gukurikiranwa mu gihe cy'imihango kugira ngo hamenyekane igihe cyiza cyo gusama. Imikorere mibi y'imisemburo mu ngirabuzimafatizo ya pituitary ishobora gutuma LH isohoka nabi. Igipimo cya LH gishobora gukoreshwa mu gusuzuma imikorere y'imisemburo ya pituitary. Agakoresho ko gusuzuma indwara gashingiye kuri immunochromatography kandi gashobora gutanga igisubizo mu minota 15.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya LH ku gace k'igeragezwa naho antibody ya IgG ya antibody y'ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya LH yanditseho fluorescence na IgG ya rabbit mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, antigen ya LH iri mu gipimo ihuzwa na antibody ya LH yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex yanyuze mu gace k'igeragezwa, yahujwe na antibody ya LH, ikora complex nshya. Urwego rwa LH rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya LH mu gipimo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw ...

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:

Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Ingero z'ibinyabutabire
.Ipaki yo gushyiramo

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO

Uburyo bwo gupima igikoresho reba igitabo cy'amabwiriza agenga ikoreshwa ry'imyunyungugu. Uburyo bwo gupima reagent ni ubu bukurikira
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Ongeramo 20μL ya serum cyangwa plasma mu gipimo cy'icyitegererezo, hanyuma uvange neza.
7. Ongeraho umuti w'icyitegererezo wa 80μL ku iriba ry'ikarita.
8. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
9. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO

.Amakuru yavuzwe haruguru ni igihe cyo gusuzuma amakuru y’iyi kit, kandi birasabwa ko buri laboratwari igomba gushyiraho igihe cyo gusuzuma amakuru ajyanye n’akamaro k’ubuvuzi ku baturage bo muri aka gace.
.Ingano ya LH iri hejuru y’urugero rw’ifatizo, kandi impinduka z’umubiri cyangwa igisubizo cy’imihangayiko ntizigomba kurebwa. Mu by’ukuri, bigomba guhuza ibimenyetso by’uburwayi.
Ibisubizo by'ubu buryo bireba gusa urwego rw'ifatizo rwashyizweho n'ubu buryo, kandi ibisubizo ntibigereranywa neza n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTIBIRAHINDURANYE ibyuma bihinduranya ibikoresho hagati y'ibikoresho bifite ubwoko butandukanye No.
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

INYANDIKO Z'IBIREGO
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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