Agapaki ko gusuzuma indwara(cyangwa seIzahabu ya Colloidal)kuri Calprotectin
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma Calprotectin (cal) ni ikizamini cya colloidal gold immunochromatographic assay cyo kumenya umubare wa kal mu mwanda w'umuntu, gifite akamaro gakomeye ko gusuzuma indwara y'amara abyimba. Iki kizamini ni reagent yo gusuzuma. Icyitegererezo cyose cyagaragaje ko kirimo ubwandu kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa. Hagati aho, iki kizamini gikoreshwa kuri IVD, ntabwo gikenewe ibikoresho by'inyongera.

INSHAMAKE
Cal ni heterodimer, igizwe na MRP 8 na MRP 14. Iboneka muri cytoplasm ya neutrophils kandi igaragara ku ruhu rw'uturemangingo tw'ingirabuzimafatizo. Cal ni poroteyine zo mu cyiciro cya acute, ifite igice gihamye neza mu gihe cy'icyumweru kimwe mu mwanda w'umuntu, yagaragaye ko ari ikimenyetso cy'indwara y'amara itera ububyimbirwe. Iyi kit ni ikizamini cyoroshye, gifite ishusho y'ubuziranenge, gipima cal mu mwanda w'umuntu, gifite ubushobozi bwo kumenya neza indwara kandi gifite umwihariko ukomeye. Ikizamini gishingiye ku ihame rya high specificit double antibodies sandwich reaction principle na gold immunochromatographic assay analysis, gishobora gutanga igisubizo mu minota 15.

IHAME RY'UBURYO BWAYO
Agace karimo agapira ka McAb gakingira calcium ku gace k’igeragezwa n’agapira ka IgG gakingira ihene ku gace k’igenzura, gafatanye na membrane chromatography mbere. Agapapuro k’igenzura gatwikiriwe na colloidal gold gafite ikimenyetso cya anti cal McAb na colloidal gold gafite ikimenyetso cya IgG gafite ikimenyetso cya IgG gafite ikimenyetso cya IgAb. Mu gihe cyo gupima icyitegererezo cyanduye, cal iri mu gipimo ivanze na colloidal gold gafite ikimenyetso cya anti cal McAb, kandi igakora ubudahangarwa bw’umubiri, kuko yemerewe kwimuka ku gace k’igerageza, cal conjugate complex ifatwa na anti cal coating McAb ku gapira maze igakora "anti cal coating McAb-cal-colloidal gold gafite ikimenyetso cya anti cal McAb", agapira k’igerageza k’amabara kagaragaye ku gace k’igerageza. Ubukana bw’ibara bufitanye isano ryiza n’ibikubiye muri cal. Icyitegererezo cya negative ntigitanga agapira k’igerageza bitewe n’uko nta colloidal gold conjugate cal complex. Uko cal yaba iri kose mu gipimo cyangwa idafite, hari umurongo utukura ugaragara ku gace k’igenzura n’akarere k’igenzura ry’ubuziranenge, gafatwa nk’amahame y’ubwiza bw’imbere mu kigo.

IBIKORESHO N'IBIKORESHO BYATANZWE
Ibice by'ipaki bya 25T:
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura
.Ingero z'ibinyabutabire: ibikoresho ni 20mM pH7.4PBS
.Dispette
.Ipaki yo gushyiramo

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA

Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
Koresha ikintu gikoreshwa mu gukusanya amasambu mashya, hanyuma uhite ugerageza ako kanya. Niba bidashoboka ko ugerageza ako kanya, bikwa ahantu hashyushye kuri 2-8°C mu gihe cy'amasaha 12 cyangwa munsi ya -15°C mu gihe cy'amezi 4.

UBURYO BWO GUKORA IKIGANIRO
1. Kuramo agakoni k'ingero, kashyizwe mu gipimo cy'amase, hanyuma usubize agakoni k'ingero inyuma, ugateke neza hanyuma uzunguze neza, ongeramo igikorwa inshuro 3. Cyangwa ukoresheje agakoni k'ingero k'amase katoranijwemo 50mg, hanyuma ushyiremo agasanduku k'ingero k'amase karimo icyitegererezo cyo gushonga, hanyuma ugateke neza.
2. Koresha icyitegererezo cy'imiyoboro ikoreshwa mu gupima umwanda, fata icyitegererezo cy'umwanda muto w'umurwayi w'impiswi, hanyuma ongeramo ibitonyanga 3 (hafi 100uL) mu muyoboro w'umwanda hanyuma uzunguze neza, ubishyire ku ruhande.
3. Kura ikarita yo gupima mu gikapu cya foil, uyishyire ku meza iringaniye hanyuma uyishyireho akamenyetso.
4. Kuraho umupfundikizo mu muyoboro w'icyitegererezo hanyuma ujugunye ibitonyanga bibiri bya mbere byakuwemo amazi, ongeramo ibitonyanga 3 (hafi 100uL) nta bitonyanga byakuwemo amazi mu buryo bugororotse hanyuma buhoro buhoro ushyire mu iriba ry'icyitegererezo cy'ikarita hamwe n'igihe cyatanzwe, tangira igihe.
5. Ibisubizo bigomba gusomwa mu minota 10-15, kandi nyuma y'iminota 15 nta gaciro bifite.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO

UBUBIKO N'UBUTABE
Iyi mashini imara amezi 24 kuva ku itariki yakozweho. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. Ntugafungure ipaki ifunze kugeza igihe uzaba witeguye gukora ikizamini.
IMIBURO N'IBYITONDERWA
1. Agakoresho kagomba kuba gafunze kandi karinzwe ubushuhe¹.
2. Ntugakoreshe icyitegererezo gishyirwa igihe kirekire cyane cyangwa gikonjeshwa kenshi kandi kigashonga kugira ngo gipimwe.
3. Ingero z'umwanda ni nyinshi cyane cyangwa ubunini bushobora gutuma ingero zavanzwe zikora nabi, nyamuneka shyira ingero zavanzwe mu cyuma gipima amazi hanyuma ufate supernatant uyipime.
4. Gukoresha nabi, urugero rurenze urugero cyangwa ruto bishobora gutuma ibisubizo bihinduka.

IMBIGAMIJWE
1. Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwagenze, ibyorezo by'indwara n'andi makuru.².
2. Iyi reagent ikoreshwa gusa mu gupima umwanda. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.