Agakoresho ko gusuzuma indwara kageneweCalprotectin(Isuzuma rya Fluorescence Immunochromatographic Assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Agakoresho ko gusuzuma indwara kageneweCalprotectin(Cal) ikwiriye mu gupima ingano y'umwanda w'umuntu. Cal ikoresheje isuzuma rya fluorescence immunochromatographic, ifite agaciro gakomeye ko gusuzuma indwara y'amara abyimba. Ingero zose zabonetse zigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
Cal ni heterodimer, igizwe na MRP 8 na MRP 14^[1]Iboneka muri cytoplasm ya neutrophils kandi igaragara ku ruhu rw'uturemangingo tw'ingirabuzimafatizo. Cal ni poroteyine zifata igihe kinini, ifite igihe gihamye mu gihe cy'icyumweru kimwe mu mwanda w'umuntu, byagaragaye ko ari ikimenyetso cy'indwara y'amara iterwa n'ububyimba.^[2-3]. Iyi kit ni ikizamini cyoroshye kandi gipima mu buryo bw'amashusho, gipima Cal mu mwanda w'umuntu, gifite ubushobozi bwo kumenya neza indwara kandi gifite ubushobozi bwo kumenya neza indwara. Ikizamini gishingiye ku buryo bworoshye bwo gusuzuma antibodies ebyiri (double antibodies) hamwe n'ikoranabuhanga ryo gusuzuma immunochromatographic assay techniques, rishobora gutanga igisubizo mu minota 15.

IHAME RY'UBURYO BWAYO
Agace gafite antibody yo gukingira irangi ya Anti-Cal ku gice cy’igeragezwa, gashyirwa kuri chromatography ya membrane mbere. Lable pad itwikiriwe na antibody yo gukingira irangi ya fluorescence mbere. Mu gupima icyitegererezo cyagaragaye, Cal iri mu gipimo ishobora kuvangwa na antibody yo gukingira irangi ya fluorescence, maze igakora uruvange rw’ubudahangarwa bw’umubiri. Uko uruvange rwemerewe kwimuka ku gice cy’igerageza, Cal conjugate complex ifatwa na antibody yo gukingira irangi ya Anti-Cal ku membrane maze igakora complex. Ubukana bwa fluorescence bufitanye isano ryiza n’ingano ya Cal. Cal iri mu gipimo ishobora kumenyekana hakoreshejwe fluorescence immunoassay analyzer.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupimisha ifite agapfunyika k'umuti wo koza amenyo ka 25T
Ingero z'ibinyabutabire 25T
Agasanduku k'ipaki 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Koresha ikintu gisukuye gikoreshwa mu gukusanya icyitegererezo cy'umwanda mushya, hanyuma ugipime ako kanya. Niba bidashoboka ko ugipima ako kanya, ubibike kuri 2-8°C mu gihe cy'iminsi 3 cyangwa munsi ya -15°C mu gihe cy'amezi 6.
2. Kuramo agati k'ingero, kashyizwe mu gipimo cy'amase, ongeramo igikorwa inshuro 3, fata ibice bitandukanye by'igipimo cy'amase buri gihe, hanyuma usubize agati k'ingero inyuma, ukaze neza hanyuma uzunguze neza, Cyangwa ukoresheje agati k'ingero katoranijwemo amase ya 50mg, hanyuma ushyiremo umuyoboro w'ingero z'amase urimo icyitegererezo cyo gushonga, hanyuma ukaze neza.
3. Koresha isuzuma ry'imiyoboro y'amazi ikoreshwa mu gupima, fata isuzuma ry'umwanda w'impiswi, hanyuma ongeramo ibitonyanga 3 (hafi 100µL) mu muyoboro w'umwanda hanyuma uzunguze neza.

Inyandiko:
1. Irinde ko ibintu bikonjesha cyangwa bigashonga.
2. Shyira ingero ku bushyuhe bw'icyumba mbere yo kuzikoresha.

UBURYO BWO GUKORA IKIGANIRO

Mbere yo gupima, soma igitabo cy'amabwiriza agenga imikorere y'igikoresho n'agasanduku k'ibikoresho.
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Kuraho umupfundikizo mu muyoboro w'icyitegererezo hanyuma ujugunye ibitonyanga bibiri bya mbere byakuwemo amazi, ongeramo ibitonyanga 3 (hafi 100uL) nta bitonyanga byakuwemo amazi mu buryo bugororotse hanyuma buhoro buhoro ubishyire mu iriba ry'icyitegererezo cy'ikarita hamwe n'ikimenyetso cyatanzwe.
7. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
8. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INDANGAGACIRO ZITEGANYIJWE
Kalori <60μg/g
Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
1. Kalori iri mu gipimo irenga 60μg/g, kandi igomba gukuraho impinduka mu miterere y'umubiri. Ibisubizo ntabwo bidasanzwe kandi bigomba gupimwa ibimenyetso by'uburwayi.
2. Ibisubizo by'ubu buryo bireba gusa ingero z'ingero zashyizweho muri ubu buryo, kandi nta kugereranya kutaziguye n'ubundi buryo.
3. Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTUGAHINDURE ibyuma bihinduranya ibikoresho hagati y'ibikoresho bifite ubwoko butandukanye No.
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

IMBIGAMIJWE
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima umwanda. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

INYANDIKO Z'IBIREGO
1.Li,G.&Y.L.Li.Isano iri hagati ya kalisiyumu n'indwara zo kwa muganga[J]. Ikinyamakuru cy'Ubuvuzi Bufatika,2007,23(15)
2.Han,W.,Xu,JM,et al. Inyigo ya calamine na lactoferrin ku barwayi barwaye indwara ya ulcerative colitis [J].Ikinyamakuru cy'Abashinwa cya Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,etc. Inyigo ku isano iri hagati ya calamine yo mu ifu n'indwara yo kubyimba amara [J]. Ikoranabuhanga n'Ubwubatsi mu bya Siyansi,2010-03,10(8)

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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