IHAME

Agace k'icyuma gipima gatwikiriwe na antigen ya microalbumin ku gace k'igeragezwa naho antibody ya IgG ya anti y'ihene ku gace k'igenzura. Agapapuro k'igenzura gatwikiriwe na microalbumin yanditseho fluorescence na IgG ya rabbit mbere y'igihe. Iyo nta albumin iri mu nkari, antibody ya monoclonal ya colloidal ifite label ya gold anti-Alb ku rupapuro rwa gold colloidal izanyura ku gace k'igenzura hamwe n'inkari kugera ku murongo wo kuzipima, kandi ihuzwe na antigen ya Alb ifite umurongo ugaragara. Kandi ibara ry'umurongo ni umwijima kurusha ibara ry'umurongo mu gace k'igenzura (C), ibi ni ibisubizo bibi. Niba inkari zirimo albumin, zizahangana na antigen ya Alb ifite label ya monoclonal kugira ngo zifatanye n'ahantu hato ha antibody kuri antibody ya colloidal ifite label ya gold anti-Alb. Uko ingano ya albumin mu nkari yiyongera, isuzuma

Ibara ry'umurongo rizarushaho kuba ryoroheje. Ibikubiye muri albumin mu nkari bishobora kugaragara mu buryo bungana ugereranije agace ko gusuzuma (T) n'agace ko kugenzura (C). Agace ko kugenzura ubuziranenge (C) n'agace ko kugenzura (R) ku gakoresho bizahora bigaragara mu gihe cyo gusuzuma, kandi ntaho bihuriye no kuba albumin y'inkari iri. Agace ko kugenzura (C) n'agace ko kugenzura (R) bishobora gukoreshwa nk'ikimenyetso cy'icyitegererezo cy'ubuziranenge bw'agakoresho.

Uburyo bwo gupima:

Mbere yo gupima, soma igitabo cy'amabwiriza agenga igikoresho n'agasanduku k'ibikoresho. Kangura ingero kugeza ku bushyuhe bw'icyumba mbere yo kubikoresha.

1. Kura ikarita yo gupima mu gafuka k'ifishi. Uyishyire ahantu hatambitse hanyuma uyishyireho ikimenyetso.

2. Fata icyitegererezo cy'inkari ukoresheje agakoresho gakoreshwa mu gusana, jugunya ibitonyanga bibiri bya mbere by'inkari. Ongeramo ibitonyanga 3 (hafi 100uL) by'inkari zidafite utubumbe hagati mu mwobo w'ikarita y'ikizamini uhagaze hanyuma utangire igihe.

3. Soma igisubizo mu minota 10-15. Ntacyo bimaze niba birengeje iminota 15.