NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa ne microalbumin antigen panzvimbo yekuyedza uye anti-rabbit IgG antibody yembudzi panzvimbo yekuyedza. Mapepa eLable pad akaputirwa ne fluorescence yakanyorwa kuti microalbumin uye rabbit IgG pachine nguva. Kana pasina albumin muweti, colloidal gold-labeled anti-Alb-labeled monoclonal antibody iri pabepa re colloidal gold ichamhanya paganda neweti ichienda kumutsetse wekuona, uye ichabatana neAlb-coated antigen ine mutsetse unooneka. Uye ruvara rwemutsetse rwakasviba kupfuura ruvara rwemutsetse munzvimbo yekuyedza (C), izvi hazvina kunaka. Kana weti iine albumin, ichakwikwidzana neAlb-coated antigen iri pamembrane kuti isunge kune nzvimbo dzeantibody dzakaganhurirwa pane colloidal gold-labeled anti-Alb-labeled monoclonal antibody. Sezvo huwandu hwealbumin muweti huchiwedzera, kuyedza

Ruvara rwemutsetse ruchawedzera kujeka. Zviri mu albumin muweti zvinogona kuonekwa zvishoma nezvishoma nekuenzanisa nzvimbo yekuona (T) nenzvimbo yekudzora (C). Nzvimbo yekudzora hunhu (C) nenzvimbo yekuyera (R) pakiti zvichagara zvichionekwa panguva yekuedzwa, uye hazvina chekuita nekuvapo kwe albumin yeweti. Nzvimbo yekudzora (C) nenzvimbo yekuyera (R) zvinogona kushandiswa sechiratidzo chemukati chekudzora hunhu hwekiti.

Maitiro Ekuyedza:

Ndapota verengai bhuku rekushandisa mudziyo uye chifukidziro chepakeji musati maedza. Nyungudutsai sampuli kusvika patembiricha yemukamuri musati mashandisa.

1. Bvisa kadhi rekuyedza kubva mubhegi refoil. Riise rakati sandara panzvimbo yakatambanuka wobva wamaka.

2. Tora weti uchishandisa pipette inoraswa, rasa madonhwe maviri ekutanga eweti. Wedzera madonhwe matatu (anenge 100uL) eweti isina mabubble pakati peburi remuenzaniso wekadhi rekuyedza wakamira wotanga nguva.

3.Verenga mhinduro yacho mumaminitsi gumi kusvika gumi nemashanu. Hazvishande kana inopfuura maminitsi gumi nemashanu.