Kiti yekuongorora chirwere()Goridhe reColloidal）yeCalprotectin
Kushandiswa kwekuongorora in vitro chete
Ndapota verengai zviri mupakeji iyi nokungwarira musati mashandisa uye teverai mirairo yacho nemazvo. Kuvimbika kwemhedzisiro yekuyedza hakugone kuvimbiswa kana paine chero kutsauka kubva pamirairo iri mupakeji iyi.

KUSHANDISA KWAKATARISIRWA
Kiti yekuongorora chirwere cheCalprotectin (cal) inzira yekuongorora chirwere checolloidal gold immunochromatographic yekuona huwandu hwecalcium kubva mutsvina yevanhu, iyo ine kukosha kwakakosha kwekuongorora chirwere chemudumbu chinozvimba. Iyi bvunzo inzira yekuongorora. Sampuli dzese dzakanaka dzinofanira kusimbiswa nedzimwe nzira. Iyi bvunzo yakagadzirirwa kushandiswa nenyanzvi dzezvehutano chete. Panguva iyi, iyi bvunzo inoshandiswa pakurapa chirwere cheIVD, hapana zvimwe zvinoshandiswa.

PFUPISO
Cal i heterodimer, inoumbwa neMRP 8 neMRP 14. Inowanikwa mu neutrophils cytoplasm uye inoonekwa pa mononuclear cell membranes. Cal i acute phase proteins, ine acute phase phase inokwana vhiki imwe chete mutsvina yevanhu, yakaonekwa sechiratidzo chezvirwere zvemudumbu. Kit iyi i test iri nyore, inoonekwa se semiqualitative iyo inoona cal mutsvina yevanhu, ine high detection sensitivity uye strong specificity. Iyo bvunzo yakavakirwa pa high specificit double antibodies sandwich reaction principle uye gold immunochromatographic assay analysis techniques, inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

NZVIMBO YEMASHURE
Mutsetse uyu une anti-cal coating McAb panzvimbo yekuyedza uye anti-rabbit IgG antibody yembudzi panzvimbo yekudzora, iyo inosungirirwa ku membrane chromatography pachine nguva. Lable pad inoputirwa ne colloidal gold yakanyorwa anti-cal McAb uye colloidal gold yakanyorwa rabbit IgG antibody pachine nguva. Pakuyedza positive sample, cal iri mu sample inosanganiswa ne colloidal gold yakanyorwa anti-cal McAb, uye inoumba immune complex, sezvo ichibvumirwa kufamba pamwe chete ne test strip, cal conjugate complex inobatwa ne anti-cal coating McAb pa membrane uye inoumba "anti-cal coating McAb-cal-colloidal gold yakanyorwa anti-cal McAb", colored test band yakaonekwa panzvimbo yekuyedza. Kusimba kweruvara kwakabatana zvakanaka ne cal content. Negative sample haiburitse test band nekuda kwekushaikwa kwe colloidal gold conjugate cal complex. Hazvinei kuti cal iripo mu sample kana kwete, pane red stripe inoonekwa panzvimbo yekuyedza uye nzvimbo yekudzora mhando, iyo inoonekwa seyemhando yemukati yebhizinesi.

ZVINHU ZVINOGONESESWA NEZVINHU
Zvikamu zvepakeji zve25T：
Kadhi rekuyedza rakaiswa foil yega yega ne desiccant
.Mienzaniso yemusanganiswa: zvinhu zviri mukati mayo i20mM pH7.4PBS
.Dispette
.Pakeji yekuisa

Zvinhu zvinodiwa asi hazvipihwe

Mudziyo wekuunganidza wemuenzaniso, nguva

MIFANANIDZO YEKUCHENGETA NOKUCHENGETA
Shandisa mudziyo wakachena unoraswa kuti uunganidze tsvina itsva, uye uiongorore nekukasika. Kana isingagoni kuongororwa nekukasika, ndapota chengeta pa2-8°C kwemaawa gumi nemaviri kana pasi pe -15°C kwemwedzi mina.

MAITIRO EKUONGORORA
1. Bvisa chimuti chekuyedza, chakaiswa mumuenzaniso wetsvina, wobva wadzosera chimuti chekuyedza, wochisunga zvakasimba wozunza zvakanaka, wodzokorora chiito katatu. Kana kushandisa chimuti chekuyedza chakatorwa sampuli yetsvina inosvika 50mg, woisa muchubhu yemuenzaniso wetsvina ine sampuli yekunyungudutsa, wobva wasunga zvakasimba.
2. Shandisai nzira yekubvisa tsvina kubva muropa, torai nzira yetsvina yakatetepa kubva kumurwere ane manyoka, mobva mawedzera madonhwe matatu (anenge 100uL) muchubhu yetsvina mozunza zvakanaka, moisa parutivi.
3. Bvisa kadhi rekuyedza kubva mubhegi refoil, riise patafura yakaenzana worimaka.
4. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akasanganiswa, wedzera madonhwe matatu (anenge 100uL) pasina bubble yakasanganiswa sampuli yakatwasuka wobva waisa zvishoma nezvishoma mutsime remuenzaniso wekadhi rine dispette yakapihwa, tanga nguva.
5. Mhedzisiro yacho inofanira kuverengwa mukati memaminitsi gumi kusvika gumi nemashanu, uye haichashandi mushure memaminitsi gumi nemashanu.

Mhedzisiro yeMiedzo neDudziro

KUCHENGETA UYE KUGADZIRIKA
Kit iyi inogara kwemwedzi makumi maviri nemana kubva pazuva rakagadzirwa. Chengeta makit asina kushandiswa pa2-30°C. Usavhure package yakavharwa kusvika wagadzirira kuita bvunzo.
YAMBIRO NEMADZIRIRO
1. Kiti yacho inofanira kuvharwa uye kudzivirirwa kubva kuhunyoro¹.
2. Usashandise sampuro inoiswa kwenguva yakareba kana inodzokororwa ichinyungudika kuti uedze
3. Samples dzetsvina dzakawandisa kana kuti ukobvu hunogona kuita kuti samples dzakasanganiswa dzisvibiswe nekadhi rekuyedza, ndapota sanganisa sampuli yakasanganiswa wotora supernatant kuti iitwe bvunzo.
4. Kusashanda zvakanaka, sampuli yakawandisa kana diki kunogona kukonzera kutsauka kwemhedzisiro.

KUGADZIRWA
1. Mhedzisiro iyi yebvunzo ndeyekutarisa kukiriniki chete, haifanirwe kushanda senzira chete yekuongororwa kwekiriniki uye kurapwa, manejimendi yekiriniki yevarwere inofanira kufungwa nezvayo pamwe chete nezviratidzo zvayo, nhoroondo yekurapa, kumwe kuongororwa kwerabhoritari, mhinduro yekurapa, epidemiology nedzimwe ruzivo².
2. Iyi reagent inoshandiswa chete pakuongororwa tsvina. Inogona kusawana mhinduro chaiyo kana ikashandiswa kune dzimwe sampuro dzakadai semate neweti nezvimwewo.