Kiti yekuongorora chirwere chepfungwaCalprotectin(Kuyedzwa kweFluorescence Immunochromatographic)
Kushandiswa kwekuongorora in vitro chete
Ndapota verengai zviri mupakeji iyi nokungwarira musati mashandisa uye teverai mirairo yacho nemazvo. Kuvimbika kwemhedzisiro yekuyedza hakugone kuvimbiswa kana paine chero kutsauka kubva pamirairo iri mupakeji iyi.

KUSHANDISA KWAKATARISIRWA
Kiti yekuongorora chirwere chepfungwaCalprotectin(Cal) yakakodzera pakuongorora huwandu hwetsvina yevanhu. Cal kuburikidza ne fluorescence immunochromatographic assay, iyo ine kukosha kwakakosha kwekuongorora chirwere chemudumbu chinozvimba. Sampuro dzese dzakanaka dzinofanira kusimbiswa nedzimwe nzira. Bvunzo iyi yakagadzirirwa kushandiswa nenyanzvi dzehutano chete.

PFUPISO
Cal i heterodimer, iyo inoumbwa neMRP 8 neMRP 14^[1]Inowanikwa mu cytoplasm ye neutrophils uye inowanikwa pa membrane yemasero e mononuclear. Cal iproteins ye acute phase, ine chikamu chakasimba chinogara kwevhiki imwe chete mutsvina yevanhu, inozivikanwa sechiratidzo chezvirwere zvemudumbu zvinozvimba.^[2-3]Kit iyi ibvunzo iri nyore, inoonekwa nehuwandu hwekuona inoona Cal mutsvina yevanhu, ine kunzwisisika kwakanyanya uye hunyanzvi hwakasimba. Bvunzo iyi yakavakirwa pakuongorora kwakawanda kwema antibodies maviri sandwich reaction principle uye fluorescence immunochromatographic assay analysis technics, iyo inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

NZVIMBO YEMASHURE
Mutsetse uyu une anti-Cal coating antibody panzvimbo yekuyedzwa, iyo inosungirirwa ku membrane chromatography pachine nguva. Lable pad inoputirwa ne fluorescence yakanyorwa kuti anti-Cal antibody pachine nguva. Pakuyedzwa positive sample, Cal iri mu sample inogona kusanganiswa ne fluorescence yakanyorwa kuti anti-Cal antibody, uye kugadzira immune mixture. Sezvo musanganiswa uchibvumirwa kufamba uchitevedza test strip, Cal conjugate complex inobatwa ne anti-Cal coating antibody pa membrane uye inoumba complex. Kusimba kwe fluorescence kunoenderana zvakanaka nehuwandu hweCal. Cal iri mu sample inogona kuonekwa ne fluorescence immunoassay analyzer.

ZVINHU ZVINOGONESESWA NEZVINHU

Zvikamu zvepakeji zve25T：
Kadhi rekuyedza rakaiswa mufoil imwe neimwe ine desiccant 25T
Mienzaniso yekuderedza mvura 25T
Chikamu chekutanga chepakeji

Zvinhu zvinodiwa asi hazvipihwe
Mudziyo wekuunganidza wemuenzaniso, nguva

MIFANANIDZO YEKUCHENGETA NOKUCHENGETA
1. Shandisa mudziyo wakachena unoraswa kuti uunganidze tsvina itsva, wobva waedza ipapo ipapo. Kana usingakwanise kuongororwa ipapo ipapo, ndapota chengeta pa2-8°C kwemazuva matatu kana pasi pe -15°C kwemwedzi mitanhatu.
2. Bvisa chimuti chekuyedza, chakaiswa mumuenzaniso wetsvina, wodzokorora chiito katatu, tora zvikamu zvakasiyana zvemuenzaniso wetsvina nguva imwe neimwe, wobva wadzosera chimuti chekuyedza, wochisunga zvakasimba wozunza zvakanaka, Kana uchishandisa chimuti chekuyedza chakatorwa sampuli yetsvina inosvika 50mg, woisa muchubhu yemuenzaniso wetsvina ine sampuli yekunyungudutsa, wobva wasunga zvakasimba.
3. Shandisai nzira yekubvisa tsvina kubva muropa, torai nzira yetsvina kubva kumurwere ane manyoka, mobva mawedzera madonhwe matatu (anenge 100µL) muchubhu yetsvina mozunza zvakanaka.

Zvinyorwa:
1.Dzivisa kuomesa nekunyungudutsa.
2. Nyautsai sampuli kusvika patembiricha yemukamuri musati mashandisa.

MAITIRO EKUONGORORA

Ndapota verenga bhuku rekushandisa chishandiso uye chifukidziro chepakeji usati waedza.
1. Isa parutivi ma reagents ese nemasamples patembiricha yemukamuri.
2.Vhura Portable Immune Analyzer(WIZ-A101), isa password yekupinda muakaunti zvichienderana nenzira yekushanda kwemudziyo, uye isa interface yekuona.
3. Skena kodhi yekuziva kuti usimbise chinhu chebvunzo.
4. Bvisa kadhi rekuyedza kubva mubhegi refoil.
5. Isa kadhi rekuyera mubhokisi remakadhi, skena QR code, uye sarudza chinhu chekuyedza.
6. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akasanganiswa, wedzera madonhwe matatu (anenge 100uL) pasina bubble yakasanganiswa yakatwasuka wobva waisa zvishoma nezvishoma muchitubu chemuenzaniso wekadhi rine dispette yakapihwa.
7. Dzvanya bhatani rekuti “standard test”, mushure memaminitsi gumi nemashanu, mudziyo unoona wega kadhi rebvunzo, unogona kuverenga mhinduro kubva pachiratidziro chemudziyo, uye wonyora/kudhinda mhinduro dzebvunzo.
8. Tarisa mirairo yePortable Immune Analyzer (WIZ-A101).

ZVINHU ZVINOTARISIRWA
Macalorie <60μg/g
Zvinokurudzirwa kuti rabhoritari yega yega igadzire huwandu hwayo hwevarwere vayo.

Mhedzisiro yeMiedzo neDudziro
1. Cal iri mumuenzaniso inodarika 60μg/g, uye inofanira kubvisa shanduko yemamiriro emuviri. Mhedzisiro yacho haina kujairika uye inofanira kuonekwa nezviratidzo zvekiriniki.
2. Mhedzisiro yenzira iyi inoshanda chete kune mareferensi akagadzwa nenzira iyi, uye hapana kuenzaniswa kwakananga nedzimwe nzira.
3. Zvimwe zvinhu zvinogona kukonzera zvikanganiso mumhedzisiro yekuona, kusanganisira zvikonzero zvehunyanzvi, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.

KUCHENGETA UYE KUGADZIRIKA
1. Kiti iyi inogara kwemwedzi gumi nemisere kubva pazuva rakagadzirwa. Chengeta makiti asina kushandiswa pa2-30°C. USAIISA muchando. Usashandise mushure mezuva rekupera.
2. Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo inoshandiswa kamwe chete inokurudzirwa kuti ishandiswe munzvimbo inodiwa (tembiricha 2-35℃, hunyoro 40-90%) mukati memaminitsi makumi matanhatu nekukurumidza sezvinobvira.
3. Musanganiswa wemuenzaniso unoshandiswa pakarepo mushure mekuvhurwa.

YAMBIRO NEMADZIRIRO
.Kit yacho inofanira kuvharwa uye kudzivirirwa kubva kuunyoro.
.Mienzaniso yese yakanaka inofanira kusimbiswa nedzimwe nzira.
.Mienzaniso yese inofanira kubatwa seinogona kusvibisa.
.USASHANDISE reagent yapera basa.
.USACHINJANA ma reagents pakati pemakiti ane nhamba dzakasiyana.
.USASHANDISA makadhi ekuyedza uye chero zvishongedzo zvinoraswa.
Kusashanda zvakanaka, kuwanda kwemuenzaniso kana kuti kushoma kunogona kukonzera kutsauka kwemhedzisiro.

KUGADZIRWA
.Sezvinoita chero bvunzo inoshandisa ma antibodies embeva, pane mukana wekuti ma antibodies emunhu anodzivirira mbeva (HAMA) apindire mumuenzaniso. Ma sampuli kubva kuvarwere vakagamuchira ma antibodies e monoclonal kuti vaongororwe kana kurapwa anogona kunge aine HAMA. Ma sampuli akadaro anogona kukonzera mhedzisiro yenhema kana yenhema.
.Mhedzisiro iyi yebvunzo ndeyekutarisa kukiriniki chete, haifanirwe kushanda senzira chete yekuongororwa kwekiriniki uye kurapwa, manejimendi ekiriniki evarwere anofanira kufungwa zvakadzama pamwe chete nezviratidzo zvavo, nhoroondo yekurapa, kumwe kuongororwa kwerabhoritari, mhinduro yekurapa, epidemiology nedzimwe ruzivo.
.Chishandiso ichi chinoshandiswa chete pakuongororwa tsvina. Chingasawana mhinduro chaiyo kana chikashandiswa kune mamwe masampuli akadai semate neweti nezvimwewo.

Hunhu hweKuita

ZVINOTAURWA
1.Li,G.&Y.L.Li. Hukama huripo pakati pecalcium nechirwere chekiriniki[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,etc. Chidzidzo chekiriniki checalamine nelactoferrin muvarwere vane ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,etc. Chidzidzo pamusoro pehukama huripo pakati pechikafu chetsvina nechirwere chemudumbu chinoputika [J]. Science Technology and Engineering,2010-03,10(8)

Kiyi yezviratidzo zvakashandiswa:

	Mudziyo wekurapa weIn Vitro Diagnostic
	Mugadziri
	Chengeta pa 2-30℃
	Zuva rekupera
	Usashandisezve
	CHENJERERO
	Bvunza Mirayiridzo Yekushandisa

Xiamen Wiz Biotech CO.,LTD
Kero: 3-4 Floor, NO.16 Building, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, China
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