Diagnostic Kit yeCalprotectin(Fluorescence Immunochromatographic Assay)
Yekushandisa in vitro diagnostic chete
Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo.Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.

ZVIRI KUSHANDISA
Diagnostic Kit yeCalprotectin(Cal) inokodzera kuongororwa kwehuwandu hwetsvina yevanhu Cal nefluorescence immunochromatographic assay, iyo ine zvakakosha zvekuongororwa kukosha kwechirwere chechirwere chechirwere.Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete.

SUMMARY
Cal is heterodimer, iyo inoumbwa neMRP 8 uye MRP 14^[1].Inowanikwa mune neutrophils cytoplasm uye inoratidzwa pane mononuclear cell membranes.Cal is acute phase mapuroteni, ine yakanyatsogadzikana chikamu chingangoita vhiki imwe mutsvina yevanhu, yakatemwa kuve chiratidzo chekuzvimba bowel chirwere.^[2-3].Iyo kiti ndeye yakapfava, inoonekwa yehuwandu bvunzo inoona Cal mutsvina yemunhu, ine yakakwirira yekuona senitivity uye yakasimba chaiyo.Muedzo wakavakirwa pahupamhi hwepamusoro hweaviri masoja ekudzivirira chirwere sandwich reaction musimboti uye fluorescence immunochromatographic assay yekuongorora matekiniki, ayo anogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

ZVINOTAURWA ZVINOITA
Iyo tambo ine anti-Cal inovhara antibody padunhu rebvunzo, iyo yakasungirirwa kune membrane chromatography pamberi.Lable pad yakaputirwa nefluorescence yakanyorwa kuti anti-Cal antibody pamberi.Paunenge uchiyedza sampuli yakanaka, iyo Cal mumuenzaniso inogona kusanganiswa nefluorescence yakanyorwa kuti anti-Cal antibody, uye kuumba musanganiswa wekudzivirira muviri.Sezvo musanganiswa uchibvumidzwa kutama uchitevedza bvunzo tambo, iyo Cal conjugate complex inotorwa neAnti-Cal coating antibody pa membrane uye mafomu akaoma.Iyo fluorescence intensity inodyidzana zvakanaka neCal content.Iyo Cal mumuenzaniso inogona kuonekwa ne fluorescence immunoassay analyzer.

REAGENTS NEZVINHU ZVINOPIWA

25T package zvikamu:
Kadhi rekuyedza rimwe nerimwe foil rakaiswa muhomwe ine desiccant 25T
Sample diluents 25T
Package isa 1

ZVINHU ZVINODIWA ASI ZVISINA KUPIWA
Sample yekuunganidza mudziyo, timer

SAMPLE KUCHENGA UYE KUCHENGA
1.Shandisa chigaba chakachena chinoraswa kuti utore sampuli yetsvina itsva, woongororwa nekukasika.Kana isingakwanisi kuongororwa nekukasira, ndapota inochengetwa pa2-8 ° C kwemazuva matatu kana pasi -15 ° C kwemwedzi mitanhatu.
2.Bvisa sampling stick, wopinza mutsvina, dzokorora chiito katatu, tora zvikamu zvakasiyana zvetsvina nguva yega yega, wozodzorera sampling stick, screw tight wozunza zvakanaka, Kana kushandisa sampling stick yanhongwa. nezve 50mg tsvina yesampuli, uye isa muchubhu yemuchubhu ine tsvina ine sample dilution, uye screw zvakasimba.
3.Shandisa pipette sampling inoraswa tora tsvina yemurwere wemanyoka, wobva wawedzera madonhwe matatu (angangoita 100µL) pachubhu yefecal sampling uye zunza zvakanaka.

Notes:
1.Dzivisa kutonhora-kunyungudika kutenderera.
2.Thaw samples kune tembiricha yekamuri usati washandisa.

ASAY PROCEDURE

Ndokumbira uverenge bhuku rekushandisa chekushandisa uye pasuru isa usati waedza.
1.Kuisa parutivi zvose reagents uye samples kune tembiricha yekamuri.
2.Vhura iyo Portable Immune Analyzer (WIZ-A101), isa iyo password password login maererano nenzira yekushanda yechiridzwa, uye pinda iyo yekuona interface.
3.Scan dentification code kuti usimbise chinhu chekuedza.
4.Bvisa kadhi rekuedza kubva mubhegi refoil.
5.Isai kadhi rekuedza mukadhi kadhi, tarisai QR code, uye sarudza chinhu chekuedza.
6.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample verticaly uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi ine dipette yakapiwa.
7.Click "standard test" bhatani, mushure memaminitsi e15, chiridzwa chichangoona kadhi rekuedza, rinogona kuverenga migumisiro kubva pachiratidziro chechiratidzo chechiridzwa, uye nyora / kudhinda mhinduro dzekuedza.
8.Rejera kune rairo yePortable Immune Analyzer(WIZ-A101).

ZVINOTARISIRWA ZVINOKOSHA
Cal <60μg/g
Zvinokurudzirwa kuti murabhoritari yega yega imise zera rayo rakajairwa rinomiririra huwandu hwevarwere vayo.

TEST RESULTS UYE DUDZIRIRO
1.The Cal mumuenzaniso yakawanda iyo 60μg / g, uye inofanira kubvisa kunze kwekuchinja kwehutano hwehupenyu.Mhedzisiro yacho zvechokwadi haina kujairika uye inofanirwa kuongororwa zviratidzo zvekiriniki.
2.Migumisiro yeiyi nzira inoshandiswa chete kune zvinyorwa zvinotaridzirwa zvakasimbiswa nenzira iyi, uye hapana kuenzaniswa kwakananga nedzimwe nzira.
3.Zvimwe zvikonzero zvinogonawo kukonzera kukanganisa mumigumisiro yekuona, kusanganisira zvikonzero zvehutano, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.

KUCHENGA UYE KUgadzikana
1.Ikiti ndeye 18 mwedzi pasherufu-hupenyu kubva pazuva rekugadzirwa.Chengetedza makiti asina kushandiswa pa2-30°C.MUSAIZE.Usashandise kupfuura zuva rekupera.
2.Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo-yekushandisa imwe chete inokurudzirwa kushandiswa pasi penzvimbo inodiwa (tembiricha 2-35 ℃, humidity 40-90%) mukati me60 mins nekukurumidza. sezvinobvira.
3.Sample diluent inoshandiswa pakarepo mushure mekuvhurwa.

YAMBIRO UYE CHENJERERO
.Ikiti inofanira kuvharwa uye kuchengetedzwa kubva pakunyorova.
.Yese mienzaniso yakanaka ichasimbiswa nedzimwe nzira.
.Mienzaniso yese ichabatwa sezvingango svibisa.
.USA shandisa expired reagent.
.USACHINANA mareagents pakati pemakiti ane akawanda akawanda Nha.
.USAshandise zvakare makadhi ebvunzo uye chero zvinhu zvinoraswa.
.Misoperation, yakawandisa kana sampuli shoma inogona kutungamirira kumhedzisiro yekutsauka.

LIMITATION
.Sezvinoita chero muyedzo unoshandisa masoja ekudzivirira embeva, mukana uripo wekukanganiswa nemasoja ekudzivirira mbeva (HAMA) mumuenzaniso.Mienzaniso kubva kuvarwere vakagamuchira gadziriro dzemonoclonal antibodies yekuongorora kana kurapwa inogona kunge iine HAMA.Mienzaniso yakadaro inogona kukonzera nhema dzakanaka kana nhema dzisina kunaka.
.Iyi mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete, haifanire kushanda seyo chete hwaro hwekuongororwa kwekiriniki uye kurapwa, varwere vekiriniki manejimendi vanofanirwa kutarisisa kwakadzama kusanganiswa nezviratidzo zvayo, nhoroondo yezvekurapa, imwe ongororo yerabhoritari, mhinduro yekurapa, epidemiology uye mamwe mashoko. .
.Iri reagent rinoshandiswa pakuongorora tsvina chete.Iyo inogona kusawana mhedzisiro chaiyo kana ichishandiswa kune mamwe masampuli senge mate uye weti uye nezvimwe.

PERFORMANCE CHARACTERISTICS

REFERENCES
1.Li,G.&Y.L.Li.Ukama huri pakati pecalcium nechirwere chekiriniki[J].The Journal of Practical Medicine,2007,23(15)
2.Han, W., Xu, JM, et al.Chidzidzo chekiriniki checalamine uye lactoferrin muvarwere vane ulcerative colitis [J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang, ZH, Guo, HB, et al.Chidzidzo pamusoro pehukama pakati pefecal calamine uye chirwere chinoputika [J].Sainzi Tekinoroji neUinjiniya,2010-03,10(8)

Kiyi yezviratidzo zvakashandiswa:

	In Vitro Diagnostic Medical Device
	Mugadziri
	Chengetedza pa2-30 ℃
	Zuva rekupera
	Usashandisezve
	KUCHENJERA
	Bvunza Mirayiridzo Yekushandisa

Xiamen Wiz Biotech CO., LTD
Kero:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel:+86-592-6808278
Fax:+86-592-6808279