NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neFOB antibody panzvimbo yekuyedza uye mbudzi anti rabbit IgG antibody panzvimbo yekudzora. Lable pad inoputirwa ne fluorescence yakanyorwa kuti anti FOB antibody uye tsuro IgG pachine nguva. Pakuyedza sampuro yakanaka, FOB antigen iri mumuenzaniso inosangana ne fluorescence yakanyorwa kuti anti FOB antibody, uye inoumba musanganiswa we immune immune. Pasi pekushanda kwe immunochromatography, complex inoyerera ichienda kune bepa rinonyudza. Kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti FOB coating antibody, inoumba complex itsva. Kana iri negative, sampuro haina hemoglobin yemunhu kana zvirimo zviri pasi, immune complex yakakwana haigone kuumbwa. Hapazovi nemutsara mutsvuku munzvimbo yekuongororwa (T). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora mhando (C) yekutonga kana paine samples dzakakwana uye kana maitiro e chromatography ari akajairwa. Inoshandiswawo se internal control standard ye reagents.

Maitiro Ekuyedza:

Ndapota verengai zviri mupakeji musati maedza.

1. Bvisa kadhi rekuyera kubva mubhegi refoil, riise patafura yakaenzana worimaka.

2. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akasanganiswa, wedzera madonhwe matatu (anenge 100uL) pasina bubble yakasanganiswa yakatwasuka yakatwasuka uye zvishoma nezvishoma mutsime remuenzaniso wekadhi rine dispette yakapihwa. Wobva watanga timer.

3. Mhedzisiro yacho inofanira kuverengwa mukati memaminitsi gumi kusvika gumi nemashanu, uye haichashandi mushure memaminitsi gumi nemashanu.