Vhitamini D ivhitamini uye zvakare ihormone yesteroid, kunyanya inosanganisira VD2 neVD3, ine chimiro chakafanana zvikuru. Vhitamini D3 neD2 zvinoshandurwa kuita 25 hydroxyl vitamin D (kusanganisira 25-dihydroxyl vitamin D3 neD2). 25-(OH) VD mumuviri wemunhu, chimiro chakasimba, huwandu hwakawanda. 25-(OH) VD inoratidza huwandu hwese hwevhitamini D, uye kugona kwekushandura kwevhitamini D, saka 25-(OH)VD inoonekwa sechiratidzo chakanakisa chekuongorora huwandu hwevhitamini D. Diagnostic Kit yakavakirwa pa immunochromatography uye inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.