Diagnostic Kit bakeng sa 25-hydroxy vithamine D (khanyang immunochromatographic thlahlobo)

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Diagnostic Kit bakeng sa 25-hydroxy vithamine D (khanyang immunochromatographic thlahlobo) Ka ho vitro tšebeliso tekotshupo feela kōpo bala sephutheloana sena kenya ka hloko pele ho sebelisa le tieo latela ditaelo. Tšepahala ha sephetho thlahlobo ke ke Guaranteed ha ho na le bopengwi efe kapa efe ho tswa ho litaelo ena kenyelletsong sephutheloana. Reretsoe SEBELISA Diagnostic Kit bakeng sa 25-hydroxy vithamine D (khanyang immunochromatographic thlahlobo) ke khanyang immunochromatographic thlahlobo bakeng sa le d ditirisanommogo ...


Product Qaqileng

Diagnostic Kit bakeng sa 25-hydroxy vithamine D (khanyang immunochromatographic thlahlobo)
Ka ho vitro tšebeliso tekotshupo feela

kōpo bala sephutheloana sena kenya ka hloko pele ho sebelisa le tieo latela ditaelo. Tšepahala ha sephetho thlahlobo ke ke Guaranteed ha ho na le bopengwi efe kapa efe ho tswa ho litaelo ena kenyelletsong sephutheloana.

Reretsoe SEBELISA
Diagnostic Kit bakeng sa 25-hydroxy vithamine D (khanyang immunochromatographic thlahlobo) ke khanyang immunochromatographic thlahlobo bakeng sa bonoa ditirisanommogo la 25-hydroxy vithamine D (25- (OH) VD) ka serum batho kapa lero la mali, eo e haholo-holo sebelisoa ho hlahloba maemo a oa vithamine ea D.It ke bo thusang tepelletse maikutlong reagent.All positive, sampole tlameha ho tiisoa ke mekgwa ya tse ling. Teko ena e reretsoe ho sebelisoa bophelo bo botle litsebi feela.

Kakaretso
vithamine D ke vithamine le ke e boetse e hormona steroid, haholo-holo ho akarelletsa VD2 le VD3, eo struction ke tsoana haholo. Vithamine d3 lego le D2 ba sokolohela 25 hydroxyl vithamine D (ho akarelletsa le 25-dihydroxyl vithamine d3 lego le D2). 25- (OH) VD ka 'mele oa motho, tsitsitseng struction, phahameng mahloriso. 25- (OH) VD bonahatsa kakaretso oa vithamine D, 'me matla a sokoloha ha vithamine D, kahoo 25- (OH) VD e nkoa e le ea pontshi molemo ka ho fetisisa bakeng sa ho hlahloba boemo ba vithamine D.The Diagnostic Kit e thehiloe immunochromatography le ka fana ka lebaka mor'a metsotso e 15.

MOLAO-MOTHEO OA TSA Tsamaiso
The lera la sesebediswa teko e tlotsitsoeng ka conjugate tsa bsa nntn le 25- (OH) VD ka sebakeng teko le pōli e ba khahlanong le mmutla IgG antibody ka sebakeng taolo. Sebaka lesupa ba tlotsitsoeng ke khanyang letšoao khahlanong 25- (OH) VD antibody le mmutla IgG esale pele. Ha leka sampole, 25- (OH) VD ka sampole kopana le khanyang tšoauoa khahlanong 25- (OH) VD antibody, le bopa motsoako 'mele. Tlas'a bohato la immunochromatography e, ho phalla e rarahaneng ka nģa ea pampiri monya, ha rarahaneng fetile sebakeng teko, The mahala fluorescent lesupa tla ho kopantswe le 25- (OH) VD ka membrane.The mahloriso ea 25- (OH) VD e mpe Correlation bakeng khanyang pontšo, 'me ea mahloriso ea 25- (OH) VD ka sampole ka lemoha ka ho khanyang immunoassay thlahlobo.

REAGENTS le thepa fa

Dikarolo 25T sephutheloana :
.Test karete bomong foil pouched le desiccant 25T
.A tharollo 25T
.B tharollo 1
.Package kenya 1

Thepa ea hlokahala hore EMPA HA A faneng
Sample pokello setshelo, pack

Sampole COLLECTION le poloko
1.The disampole lekoa ka serum, heparin anticoagulant lero la mali kapa EDTA anticoagulant lero la mali.

2.According ho dithekeniki tekanyetso bokella sampole. Serum kapa lero la mali sampole ka 'na a refrigerated ka 2-8 ℃ bakeng 7days le cryopreservation tlase -15 ° C bakeng sa likhoeli tse 6.
3.All sampole lokela ho qoba hatsele-qhibilihisa lipotoloho.

Thlahlobo Tsamaiso
The teko Tsamaiso ea seletsa sa bona immunoanalyzer pukwanana. Tsamaiso e reagent teko e tjena

1.Lay thoko reagents tsohle le disampole ho mocheso kamore.
2.Open e nkehang habobebe 'mele semanolli (Wiz-A101), kenya tlalehong phasewete tsa ho kena ho ea ka mokhoa tšebetso ea seletsa sa,' me ba kene segokanyimmediamentsi sa sebolokigolo ba bonoa.
3.Scan le dentification khoutu ho tiisetsa ntlha ya teko.
4.Take tsoa karete teko tloha mokotla foil.
5.Insert teko karete ka sekotjana sa karete, Scan le khoutu QR, 'me beha ntho teko.
6.Add 30μL serum kapa lero la mali sampole ho tharollo e, 'me kopanya hantle.
7.Add 50μL B tharollo ho motsoako ka holimo, 'me kopanya hantle.
8 .Siea motsoako bakeng sa metsotso e 15.
9.Add 80μL motsoako ho latsoa hantle ya karete.
10.Click le "molao-motheo teko" konopo, ka mor'a metsotso e 10, seletsa se tla ka tsela e iketsang hlokomela karete teko, ho ka bala sephetho ho tswa ho skrine ponahatso ea seletsa sa, 'me tlaleho / условия: diphetho tekong.
11.Refer ho thupelo ea nkehang habobebe 'mele semanolli (Wiz-A101).

Lebeletsoe litekanyetso
25- (OH) VD tloaelehile mefuta e fapaneng: 30-100ng / ML

E khothalletsa hore e mong le e laboratori ea theha tlwaelehileng tsa lona kgatlhanang emetseng baahi ba eona ba le mamello.

Teko RESULTS le tlhaloso
.The hodimo ya data e moo ho buuoang karohano thehilwe bakeng sa boitsebiso ba bonoa ba Kit ena, 'me e etsa tlhahiso ea hore e mong le e laboratori ea lokela ho theha bolela karohano mabapi le bohlokoa ea tleleniki ea baahi ba sebakeng sena.

.The mahloriso ea 25- (OH) VD e phahame ho feta fapaneng ya referense, 'me liphetoho fisioloji kapa ka karabelo khatello ea kelello e lokela ho excluded.Indeed e sa tloaelehang, ba lokela ho kopana litleleniki tsa letšoao tepelletse maikutlong.
.The diphetho mokhoa ona ke ho tshwanetseng feela ho moo ho buuoang mefuta e fapaneng e thehiloeng ke mokhoa ona, 'me liphello e ba tse se ka kotloloho tšoana le mekhoa e meng.
Lintlha .Other Hape ho ka etsa hore liphoso tsa sephetho ba bonoa, ho akarelletsa mabaka a botekgeniki, liphoso tshebetso le lintho tse ling sampole.

Storage le botsitso
.The Kit ke likhoeli tse 18 raka, athe-bophelo ho tloha letsatsing la proizvodnje. Boloka lebokosana le sa sebelisoeng ka 2-30 ° C. U SE KE UA hatsele. U se ke ua sebelisa ka nģ'ane letsatsi tla felloa ke matla ka.

.Do sa bula mokotla tiisitsoe ho fihlela u se u loketse ho etsa teko, mme ba se nang balekane-tšebeliso teko e etsa tlhahiso ea hore a ka sebelisoa ka tlas'a tikoloho tlhokigang (mocheso 2-35 ℃, mongobo 40-90%) ka hare ho 60 mins kapele kamoo ho khoneha.
.Sample diluent e sebediswa hang-hang ka mor'a hore a ile a bula.

TEMOSO E LE mehato
.The Kit lokela ho tiisitsoe 'me o ile a sireletsa khahlanong le mongobo.

.All exemplar positive, le tla tiisitsoe ka oona ke mekgwa ya tse ling.
Exemplar .All tla tšoaroa joaloka pollutant hlahelang.
.DO SE sebelisa e khutlile reagent.
.DO SE fapanyetsana reagents har'a lebokosana le ngata fapaneng ha ..
.DO SE sebedisa botjha tumellano likarete teko le ya disebediswa tse kathoko efe kapa efe lahloang.
.Misoperation, ka tsela e feteletseng kapa ho honyenyane sampole ho ka etsa hore bo hlahe bopengwi.

L etsisa
.As le thlahlobo sebelisa lisireletsi efe kapa efe toeba, monyetla e teng bakeng sa tshitsiso ke lisireletsi batho-ba khahlanong le toeba (Hama) ka disampole le. Exemplar ar tloha bakuli ba amohetse ka litokisetso tsa lisireletsi monoclonal bakeng tepelletse maikutlong kapa phekolo e ka ba le Hama. Exemplar joalo ka 'na ea etsa hore ba bohata liphello tse ntle kapa bohata negative.

.This lebaka teko e feela ya referense litleleniki tsa, ha aa lokela ho sebeletsa e le motheo oa feela phumano ea tleleniki le kalafo, le bakuli ba nang taolo ya tsa tleleniki e lokela ho ba le felletseng la ho nahanela ho kopantswe le matšoao a lona, histori ea bongaka, laboratori ea ho hlahloba ka tse ling, kalafo le karabelo, Epidemiology le tlhahisoleseding e meng .
Reagent .This e feela sebediswa bakeng sa serum le lero la mali le liteko. Ho ka 'na ha se fumana lebaka nepahetse ha sebelisoa bakeng sa ho disampole tse ling tse kang mathe le moroto le joalo-joalo

Performance litšobotsi

Linearity 5 ng, / ML ho 120 ka ng / ML a lekanyelitsoeng kheloha: -15% ho + 15%.
Guttate Correlation coefficient: (a r) ≥0.9900
ho nepahala ha eona Sekhahla hlaphoheloa tla ba ka hare ho 85% - 115%.
Repeatability CV≤15%
Khethehileng
(Ha ho na lintho tse ka ka interferent e lekiloeng kena-kenana ka ho thlahlobo e)
Interferent Interferent mahloriso
haemoglobin 200μg / ML
transferrin 100μg / ML
Horse radish peroxidase 2000μg / ML
vithamine d3 lego 50mg / ML
vithamine D 50mg / ML

R EFERENCES

1.Hansen JH, et-al.HAMA sitisoe le Murine Monoclonal Antibody-Thehiloe Immunoassays [J] .J tsa Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.The Hantle Heterophilic dianthibodi di hlahelle le Karolo ka Immunoassay sitisoe [J] .J tsa Clin Immunoassay, 1992,15: 108-114.

Senotlolo sa ho matshwaonyana sebediswa:

 t11-1 Ka vitro Diagnostic Medical sesebelisoa
 TT-2 Manufacturer
 TT-71 Store ka 2-30 ℃
 TT-3 Letsatsi la pheletso
 TT-4 U se ke Ua sebedisa botjha tumellano
 TT-5 temoso
 TT-6 Rerisanang Instructions Ka Sebelisa

Xiamen Wiz Biotech CO, LTD.
Address: 3-4 Floor, NO.16 Building, Bio-ya kalafi kokoano, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, China
Tel: + 86-592-6808278
Fax: + 86- 592-6808279


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