Ikhithi yokuxilonga(IsiNgesi)Igolide yeColloidal）yeCalprotectin
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Isixhobo sokuxilonga seCalprotectin (cal) sisixhobo sovavanyo lwe-colloidal gold immunochromatographic sokufumanisa ubungakanani be-cal kwindle yomntu, esinexabiso elibalulekileyo lokuxilonga isifo samathumbu esidumbayo. Olu vavanyo luyi-reagent yokuhlola. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zempilo kuphela. Okwangoku, olu vavanyo lusetyenziselwa i-IVD, akukho zixhobo zongezelelweyo ezifunekayo.

ISISHWANKATHELO
I-Cal yi-heterodimer, eyenziwe yi-MRP 8 kunye ne-MRP 14. Ifumaneka kwi-neutrophils cytoplasm kwaye ibonakaliswa kwiimembrane zeseli ze-mononuclear. I-Cal yiprotheni yesigaba esibukhali, inesigaba esizinzileyo malunga neveki enye kwilindle lomntu, ifunyaniswa ukuba luphawu lwesifo samathumbu esivuthayo. Le khithi luvavanyo olulula, olubonakalayo olulinganiselayo olubona i-cal kwilindle lomntu, lunobuntununtunu bokufumanisa obuphezulu kunye nobuchwephesha obuqinileyo. Uvavanyo olusekelwe kwi-high specificit double antibodies sandwich reaction principle kunye ne-gold immunochromatographic assay analysis techniques, lunokunika iziphumo kwimizuzu eli-15.

UMGAQO WENKQUBO
I-strip ine-anti-cal coating McAb kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula, ebotshelelwe kwi-membrane chromatography kwangaphambili. I-Lable pad igqunywe ngegolide ye-colloidal ebhalwe i-anti-cal McAb kunye ne-colloidal gold ebhalwe i-rabbit IgG antibody kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-cal kwisampuli ihlanganiswe negolide ye-colloidal ebhalwe i-anti-cal McAb, kwaye yenze i-immune complex, njengoko ivunyelwe ukuba ihambe ecaleni kwe-test strip, i-cal conjugate complex ibanjwa yi-anti-cal coating McAb kwi-membrane kwaye yenze i-complex ethi "anti-cal coating McAb-cal-colloidal gold ebhalwe i-anti-cal McAb", ibhendi yovavanyo enemibala yavela kwindawo yovavanyo. Ubungakanani bombala buhambelana kakuhle nomxholo we-cal. Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Nokuba i-cal ikhona kwisampuli okanye ayikho, kukho umgca obomvu obonakalayo kwindawo yokujonga kunye nommandla wolawulo lomgangatho, othathwa njengemigangatho yangaphakathi yeshishini yomgangatho.

IZINTO EZINIKEZELWAYO NEZIXHOBO
Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo ngalinye lifakwe ifoyile ngesixhobo sokucoca
.Iisampulu zokuxuba: izithako ziyi-20mM pH7.4PBS
.Dispette
.Iphakheji efakiweyo

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA

Isitya sokuqokelela iisampulu, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
Sebenzisa isitya esicocekileyo esilahlwayo ukuze uqokelele isampuli yendle entsha, kwaye uvavanywe ngoko nangoko. Ukuba awukwazi ukuvavanywa ngoko nangoko, nceda ugcine kwi-2-8°C iiyure ezili-12 okanye ngaphantsi kwe-15°C iinyanga ezi-4.

INKQUBO YOVAVANYO
1. Khupha intonga yokuvavanya, efakwe kwisampuli yendle, uze uyibuyisele intonga yokuvavanya, uyiqinise uze uyishukumise kakuhle, uphinde wenze loo nto izihlandlo ezi-3. Okanye usebenzisa intonga yokuvavanya ekhethwe malunga nesampuli yendle engama-50mg, uze ufake ityhubhu yesampuli yendle equlethe isampuli yokunyibilikisa, uze uyijije kakuhle.
2.Sebenzisa isampuli yepayipi elahlwayo, thatha isampuli yelindle elincinci kumguli orhudayo, uze wongeze amathontsi amathathu (malunga ne-100uL) kwityhubhu yokuvavanya ilindle uze ushukumise kakuhle, ubeke ecaleni.
3.Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.
4. Susa isiciko kwityhubhu yesampulu uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi amathathu (malunga ne-100uL) isampuli exutyiweyo engaxutyiweyo ngokuthe nkqo uze kancinci ungene kwisampuli equlathe ikhadi nge-dispette enikiweyo, ixesha lokuqala.
5. Isiphumo kufuneka sifundwe kwimizuzu eli-10-15, kwaye asisebenzi emva kwemizuzu eli-15.

IZIPHUMO ZOVAVANYO NOKUTOLIKWA

UKUGCINWA NOKUZINZISA
Le khithi ihlala iinyanga ezingama-24 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. Musa ukuyivula ingxowa evaliweyo ude ulungele ukwenza uvavanyo.
IZILUMKISO NEZILUMKISO
1. Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama¹.
2. Musa ukusebenzisa isampuli ebekwe ixesha elide kakhulu okanye ephindaphindwayo yokuqandisa nokunyibilikisa ukuze kuvavanywe
3. Iisampuli zelindle zigqithisile okanye ubukhulu bunokwenza iisampuli ezixutyiweyo zibe yikhadi lovavanyo elingcolileyo, nceda ufake isampuli exutyiweyo kwindawo enye uze uthathe i-supernatant ukuze ivavanywe.
4. Ukusebenza kakubi, isampuli egqithisileyo okanye encinci kunokukhokelela ekuphambukeni kweziphumo.

UMDA
1. Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sokuphela kokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.².
2. Le reagent isetyenziswa kuphela kuvavanyo lwelindle. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.