Ikhithi yokuxilonga ye-microalbumin yomchamo

(Uvavanyo lwe-Fluorescence Immunochromatographic)

Ukusetyenziswa kokuxilongwa kwi-vitro kuphela

Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO

Ikhithi yokuxilonga ye-microalbumin yomchamo (i-Fluorescence Immunochromatographic Assay) ifanelekile ekufumaneni ubungakanani be-microalbumin kumchamo womntu ngovavanyo lwe-fluorescence immunochromatographic, olusetyenziswa kakhulu ekuxilongeni isifo sezintso. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO

I-Microalbumin yiproteni eqhelekileyo efumaneka egazini kwaye ayifumaneki kakhulu kumchamo xa isetyenziswa ngendlela eqhelekileyo. Ukuba kukho inani elincinci kumchamo, i-Albumin ingaphezulu kwe-20 micron / mL, ikwi-microalbumin yomchamo, ukuba inganyangwa ngexesha elifanelekileyo, inokulungisa ngokupheleleyo i-glomeruli, isuse i-proteinuria, ukuba ayinyangwa ngexesha elifanelekileyo, inokungena kwisigaba se-uremia. Ukwanda kwe-microalbumin yomchamo kubonakala kakhulu kwi-nephropathy yesifo seswekile, uxinzelelo lwegazi oluphezulu kunye ne-preeclampsia ekukhulelweni. Le meko inokuxilongwa ngokuchanekileyo ngexabiso le-microalbumin yomchamo, kunye nokwenzeka kwayo, iimpawu kunye nembali yezonyango. Ukufunyanwa kwangoko kwe-microalbumin yomchamo kubaluleke kakhulu ukuthintela nokulibazisa ukuphuhliswa kwe-nephropathy yesifo seswekile.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-ALB antigen kwindawo yovavanyo kunye ne-antibody yebhokhwe ye-anti rabbit IgG kwindawo yokulawula. I-Marker pad igqunywe nge-antibody ye-ALB uphawu lwe-fluorescence kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli, i-ALB kwisampuli idibana ne-antibody ye-ALB uphawu lwe-fluorescence, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, i-free fluorescent marker iya kudityaniswa ne-ALB kwi-membrane. Uxinzelelo lwe-ALB lulwalamano olubi lwesignali ye-fluorescence, kwaye uxinzelelo lwe-ALB kwisampuli lunokubonwa ngovavanyo lwe-fluorescence immunoassay.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：

Ikhadi lovavanyo lifakwe ifoyile nganye nganye ene-desiccant engama-25T

Iphakheji efakiweyo 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA

Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO

1. Iisampulu ezivavanyiweyo zinokuba ngumchamo.
2. Iisampuli zomchamo ezintsha zingaqokelelwa kwisitya esicocekileyo nesilahlwayo. Kucetyiswa ukuba kuvavanywe iisampuli zomchamo kwangoko emva kokuba ziqokelelwe. Ukuba iisampuli zomchamo azinakuvavanywa ngoko nangoko, nceda uzigcine ku-2-8.℃, kodwa kuyacetyiswa ukuba ungagciniZibeke ngaphezulu kweeyure ezili-12. Musa ukuzishukumisa isitya. Ukuba kukho inkunkuma ezantsi kwesitya, thatha i-supernatant ukuze uyivavanye.
3. Zonke iisampuli ziphepha imijikelo yokuqandisa nokunyibilikisa.
4. Nyibilikisa iisampulu zibe kubushushu begumbi ngaphambi kokuba uzisebenzise.