I-Tf ifumaneka kakhulu kwi-plasma, umxholo ophakathi umalunga ne-1.20 ~ 3.25g/L. Kubantu abaphilileyo, akukho ndawo ibonakalayo. Xa ukopha kwindlela yokugaya ukutya, i-Tf esegazini ingena kwindlela yokugaya ukutya kwaye ikhutshwe kunye nendle, ininzi kwindle yabantu abanesifo sokopha esiswini. Ke ngoko, i-Tf yendle idlala indima ebalulekileyo ekufumaneni ukopha kwendle. Le khithi luvavanyo olulula nolubonakalayo olubona i-Tf kwindle yabantu, inobukrelekrele bokufumanisa kunye nokuchaneka okunamandla. Uvavanyo olusekelwe kwi-high specificit double antibodies sandwich reaction principle kunye ne-gold immunochromatographic assay analysis techniques, lunokunika iziphumo kwimizuzu eli-15.