UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-FOB antibody kwindawo yovavanyo kunye ne-IgG antibody yebhokhwe elwa nomvundla kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-antiFOB antibody kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli echanekileyo, i-antigen ye-FOB kwisampuli idibana ne-antiFOB antibody ebhalwe nge-fluorescence, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-antiFOB coating antibody, yenza i-complex entsha. Ukuba i-negative, isampuli ayinayo i-hemoglobin yomntu okanye umxholo uphantsi, ii-immune complex ezaneleyo azinakwenziwa. Akuyi kubakho mgca obomvu kwindawo yokufumanisa (T). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) wokugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo:

Nceda ufunde iphakheji engezantsi ngaphambi kokuba uyivavanye.

1.Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.

2. Susa isiciko kwityhubhu yesampulu uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi amathathu (malunga ne-100uL) isampuli exutyiweyo engenamaqamza ngokuthe nkqo kwaye kancinci kancinci kwiqula lesampulu yekhadi ene-dispette enikiweyo. Emva koko qala i-timer.

3.Isiphumo kufuneka sifundwe kwimizuzu eli-10-15, kwaye asisebenzi emva kwemizuzu eli-15.