Sicinga ukuba oko abathengi bakucingayo, ukungxamiseka kokungxamiseka kokuthatha amanyathelo ngokwembono yomthengi, ukuvumela umgangatho ophezulu, ukunciphisa iindleko zokucubungula, uluhlu lwamaxabiso lufanelekile ngakumbi, kunike abathengi abatsha nabaphelelwe lixesha inkxaso kunye nokuqinisekiswa kweeNkampani zoMveliso zeCORONA Virus Disease Rapid Antibody Test Kit, Masisebenzisane ukuze senze ikamva elihle kunye. Siyakwamkela ngokunyanisekileyo ukuba undwendwele inkampani yethu okanye uthethe nathi ukuze sisebenzisane!
Sicinga ukuba oko abathengi bakucingayo, ukungxamiseka kokuthatha amanyathelo ngokwezinto ezifunwa ngumthengi ngokwembono yakhe, ukuvumela umgangatho ophezulu, ukunciphisa iindleko zokucubungula, uluhlu lwamaxabiso lufanelekile ngakumbi, kuzuze abathengi abatsha nabaphelelwe lixesha inkxaso kunye nokuqinisekiswa.Ikhithi yoVavanyo lwe-Antibody yaseTshayina kunye neKiti yoVavanyo lokuHlola, Sisoloko sibambelele kumgaqo wenkampani othi “ukunyaniseka, amava, ukusebenza kakuhle kunye nokuvelisa izinto ezintsha”, kunye nemisebenzi ethi: mabavumele bonke abaqhubi bonwabele ukuqhuba kwabo ebusuku, mabavumele abasebenzi bethu baqonde ixabiso labo lobomi, kwaye babe namandla kwaye bancede abantu abaninzi. Sizimisele ukuba ngumdibanisi wemarike yethu yemveliso kunye nomboneleli wenkonzo omnye kwimarike yethu yemveliso.
Ikhithi yokuxilonga ye-Antibody to Helicobacter Pylori(Uvavanyo lwe-Fluorescence Immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Ikhithi yokuxilonga ye-Antibody to Helicobacter Pylori (Uvavanyo lwe-Fluorescence Immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokufumanisa ubungakanani be-HP antibody kwi-serum okanye kwi-plasma yomntu. Eli lixabiso elibalulekileyo lokuxilongwa kwezifo zesisu. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zempilo kuphela.

ISISHWANKATHELO
Usulelo lwesisu i-helicobacter pylori lunxulumene kakhulu ne-gastritis engapheliyo, isilonda sesisu, i-adenocarcinoma yesisu, i-lymphoma enxulumene ne-mucosa yesisu, izinga losulelo lwe-Hp ylori elimalunga ne-90% kwizigulane ezine-gastritis, isilonda sesisu, isilonda se-duodenal kunye nomhlaza wesisu. I-WHO ichonge i-h. pylori njengohlobo lokuqala lwe-cancer-coming factor. Luphawu oluyingozi lomhlaza wesisu. Ukufunyanwa kwe-H. pylori kubaluleke kakhulu ekuchongweni kosulelo lwe-h. pylori.

UMGAQO WENKQUBO
I-membrane yesixhobo sovavanyo igqunywe nge-HP antigen kwindawo yovavanyo kunye ne-antibody yebhokhwe ye-IgG antibody kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-HP antigen kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-HP antibody kwisampuli idibana ne-fluorescence ebhalwe nge-HP antigen, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-HP coating antigen, yenza i-complex entsha. Inqanaba le-HP-Ab lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-HP-Ab kwisampuli lunokubonwa ngovavanyo lwe-fluorescence immunoassay.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo lifakwe ifoyile nganye nganye ene-desiccant engama-25T
Iisampulu zokuxuba i-25T
Iphakheji efakiweyo 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1.Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye yeplasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezisi-7 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-6.
3.Zonke iisampuli ziphephe imijikelo yokuqandisa nokunyibilikisa.

INKQUBO YOVAVANYO
Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesihlomelo sephakheji ngaphambi kokuvavanya.
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwingxowa yefoyile.
5.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
6. Yongeza isampuli yeserum okanye iplasma engama-20μL kwisampuli ye-diluent, uze udibanise kakuhle.
7. Yongeza isisombululo sesampulu se-80μL kwisampulu yomthombo wekhadi.
8.Cofa iqhosha elithi “uvavanyo oluqhelekileyo”, emva kwemizuzu eli-15, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, singafunda iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
9. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IINQOBO EZILINDELEKILEYO
I-HP-Ab<10
Kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwayo oluqhelekileyo olumele inani lezigulana zayo.

IZIPHUMO ZOVAVANYO NOKUTOLIKWA
.Idatha engentla yimiphumo yovavanyo lwe-HP-Ab reagent, kwaye kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwamaxabiso okufumanisa i-HP-Ab afanelekileyo kuluntu olukulo mmandla. Iziphumo ezingentla zezokujonga kuphela.
Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe kule ndlela, kwaye akukho kuthelekiswa ngokuthe ngqo nezinye iindlela.
Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
1. Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
2. Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza okukhulu.
3. Isampulu yomxube isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo maziqinisekiswe ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
.MUSA ukutshintshanisa ii-reagents phakathi kweekiti ezinenani elahlukileyo.
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwe-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.

IIMPAWU ZOKUSEBENZA

RIINGXELO
1.Shao, JL&F.Wu. Inkqubela phambili yakutshanje kwiindlela zokufumanisa iHelicobacter pylori[J]. Ijenali yeGastroenterology kunye neHepatology, 2012,21(8):691-694
2.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.
Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

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