UKUSETYENZISWA OKUJOLISWEYO

Ikhithi yokuxilonga yeCalprotectin (cal) luvavanyo lwe-colloidal gold immunochromatographic lokufumanisa ubungakanani be-cal kwindle yomntu, olunezixhobo ezibalulekileyo zokuchonga
ixabiso lesifo samathumbu esidumbileyo. Olu vavanyo luyi-reagent yokuvavanya. Zonke iisampulu ezibonisa ukuba zilungile kufuneka zibe
iqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zempilo kuphela.
Okwangoku, olu vavanyo lusetyenziselwa i-IVD, akukho zixhobo ezongezelelweyo ezifunekayo.
ISISHWANKATHELO
I-Cal yi-heterodimer, eyenziwe yi-MRP 8 kunye ne-MRP 14. Ifumaneka kwi-neutrophils cytoplasm
kwaye ibonakaliswa kwiimembrane zeseli ze-mononuclear. I-Cal yiproteni yesigaba esibukhali, izinzile kakuhle
Emva kweveki enye kwilindle lomntu, kuye kwafunyaniswa ukuba luphawu lwesifo samathumbu esidumbayo.
Le khithi luvavanyo olulula, olubonakalayo nolulinganiselayo olubona iikhalori kwilindle lomntu, lunokubonwa okuphezulu
uvakalelo kunye nokuchaneka okunamandla. Uvavanyo olusekelwe kwisandwich yee-antibodies ezimbini ezicacileyo eziphezulu
umgaqo wokuphendula kunye neendlela zohlalutyo lwe-immunochromatographic assay, inokunika iziphumo
kwimizuzu eli-15.
UMGAQO WENKQUBO
I-strip ine-McAb ekhusela i-cal kwindawo yovavanyo kwaye i-anti-rabbit IgG antibody yebhokhwe ilawulwa.
ummandla, obotshelelwe kwi-membrane chromatography kwangaphambili. I-Lable pad igqunywe yi
igolide ye-colloidal ebhalwe i-anti cal McAb kunye ne-colloidal gold ebhalwe i-rabbit IgG antibody kwangaphambili.
Xa kuvavanywa isampuli ephilayo, i-cal kwisampuli ifakwe igolide ye-colloidal ebhalwe i-anti cal McAb,
kwaye zenze i-immune complex, njengoko ivunyelwe ukuba ihambe ecaleni komcu wovavanyo, i-cal conjugate
I-complex ibanjwa yi-anti-cal coating McAb kwi-membrane kwaye yenze i-“anti-cal coating
I-McAb-cal-colloidal yegolide ebhalwe i-anti cal McAb” complex, ibhendi yovavanyo enemibala yavela kuvavanyo
ummandla. Ubungakanani bombala buhambelana kakuhle nomxholo we-cal. Isampuli engalunganga ayibonisi
velisa ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Nokuba i-cal iyintoni na
ukuba ikhona okanye ayikho kwisampulu, kukho umgca obomvu obonakalayo kwindawo yesalathiso kunye nolawulo lomgangatho
ummandla, othathwa njengemigangatho yomgangatho wangaphakathi weshishini.

Uvavanyo lwethu lwe-CAL ngumzi-mveliso wokuqala ukufumana i-CFDA eTshayina. Sele sithumela kumazwe amaninzi kwaye zonke iimpendulo ziyancomeka.

Wamkelekile ukuba unxibelelane nathi ukuze ufumane iinkcukacha ezithe vetshe.