ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-anti-AFP antibody endaweni yokuhlola kanye ne-anti-IgG antibody yembuzi endaweni yokulawula. Iphedi yelebula imbozwe nge-anti-AFP antibody ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-AFP kusampula ihlangana ne-anti-AFP antibody ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ohlangothini lwephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, ihlanganiswe ne-anti-AFP coating antibody, yakha i-complex entsha. Izinga le-AFP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-AFP kusampula kungatholakala nge-fluorescence immunoassay assay.

Inqubo Yokuhlola

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3. Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma i-plasma engu-20μL ku-diluent yesampula, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL emthonjeni wesampula wekhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).