Ikhithi Yokuxilonga(I-1965)Igolide le-Colloidal）i-Luteinizing Hormone
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Le khithi isetshenziselwa ukuthola amazinga e-Luteinizing Hormone (LH) kumasampula omchamo womuntu. Ifanele ukubikezela isikhathi sokuvuthwa kweqanda. Qondisa abesifazane abaseminyakeni yokuzala ukuthi bakhethe isikhathi esingcono kakhulu sokukhulelwa, noma uqondise ukuvimbela ukukhulelwa okuphephile. Lokhu kuhlolwa kuyi-reagent yokuhlola. Wonke amasampula abonisa ukuthi akhona kumele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela. Okwamanje, lokhu kuhlolwa kusetshenziselwa i-IVD, akudingeki amathuluzi engeziwe.

USAYIZI WEPAKETHE

Ikhithi eli-1/ibhokisi, amakhithi ayi-10/ibhokisi, amakhithi angama-25,/ibhokisi, amakhithi ayi-100/ibhokisi.

ISIFINYEZO
I-LH iyi-hormone ye-glycoprotein ekhishwa yi-pituitary gland, ikhona egazini lomuntu nasemchameni, engakhuthaza ukukhishwa kwamaqanda avuthiwe esibelethweni. I-LH ikhishwa phakathi nesikhathi esiphakathi sokuya esikhathini, futhi lapho ifinyelela inani eliphakeme le-LH, yakhuphuka ngokushesha yafika esicongweni sama-25-200 miu/mL kusukela ezingeni eliyisisekelo lama-5-20 miu/mL. Ukuhlushwa kwe-LH emchameni kuvame ukukhuphuka kakhulu emahoreni angama-36-48 ngaphambi kokuvuthwa, kufinyelele inani eliphakeme emahoreni ayi-14-28. Inani le-LH emchameni livame ukukhuphuka kakhulu emahoreni angama-36 kuya kwangu-48 ngaphambi kokuvuthwa, futhi lifinyelele inani eliphakeme emahoreni ayi-14-28, ulwelwesi lwe-follicular lwaqhuma cishe emahoreni ayi-14 kuya kwangu-28 ngemva kokuvuthwa futhi lwakhipha amaqanda avuthiwe. Abesifazane bavame ukuvunda kakhulu esicongweni se-LH zingakapheli izinsuku eziyi-1-3, ngakho-ke, ukutholwa kwe-LH emchameni kungasetshenziswa ukubikezela isikhathi sokuvuthwa.^[1]. Le khithi isekelwe kubuchwepheshe bokuhlaziya i-colloidal gold immune chromatography ukuthola i-antigen ye-LH kumasampula omchamo womuntu, okunganikeza umphumela zingakapheli imizuzu eyi-15.

INQUBO YOKUHLOLA
1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
2. Lahla isampula yokuqala yamaconsi amabili, engeza amaconsi amathathu (cishe i-100μL) ngaphandle kwesampula yebhamuza ngokuqondile bese kancane kancane uyifaka emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.
3. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.