Kit sokuxilonga ukuze 25-hydroxy Vitamin D (fluorescence immunochromatographic Assay)

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Kit sokuxilonga ukuze 25-hydroxy Vitamin D (fluorescence immunochromatographic Assay) Ukuze ngaphandle kwesibeletho ukusetshenziswa zokuxilonga kuphela Sicela ufunde lokhu iphakethe ufaka ngokucophelela ngaphambi ukusebenzisa futhi ngokuqinile landela imiyalelo. Ukuthembeka Assay Imiphumela akukwazi ukuqinisekiswa uma kukhona ngeziphambeko kusukela imiyalelo kule Faka iphakethe. Okuhloswe UKUSETSHENZISWA Yokucwaninga Kit for 25-hydroxy Vitamin D (fluorescence immunochromatographic Assay) kuyinto Assay fluorescence immunochromatographic ngokuba d ambalwa ...


umkhiqizo Imininingwane

Kit sokuxilonga ukuze 25-hydroxy Vitamin D (fluorescence immunochromatographic Assay)
Ukuze ngaphandle kwesibeletho ukusetshenziswa zokuxilonga kuphela

Sicela ufunde lokhu iphakethe ufaka ngokucophelela ngaphambi ukusebenzisa futhi ngokuqinile landela imiyalelo. Ukuthembeka Assay Imiphumela akukwazi ukuqinisekiswa uma kukhona ngeziphambeko kusukela imiyalelo kule Faka iphakethe.

Okuhloswe UKUSETSHENZISWA
Yokucwaninga Kit for 25-hydroxy Vitamin D (fluorescence Assay immunochromatographic) kuyinto Assay fluorescence immunochromatographic ngenxa ukuthola ambalwa of 25-hydroxy Vitamin D (25 (OH) vd) e serum womuntu noma uketshezi olungenambala olusegazini, okuyinto ikakhulu kusetshenziswa ukulinganisa amazinga vitamin D.It kuyinto asizayo uphethwe reagent.All isampula omuhle kumele kuqinisekiswa nezinye izindlela. Lokhu kuhlola senzelwe kwezempilo ukusetshenziswa professional kuphela.

UKUFINGQA
Vitamin D amavithamini futhi hormone steroid, ikakhulukazi kuhlanganise VD2 futhi VD3, ogama wokwakha kuyafana. Vitamin D3 no D2 eziguqulelwa 25 hydroxyl vitamin D (kufaka 25-dihydroxyl Vitamin D3 no D2). 25 (OH) vd emzimbeni womuntu, kwakhiwe ezinzile, emakamu eliphezulu. 25 (OH) vd ibonisa isamba uvithamini D, kanye nekhono ukuguqulwa uvithamini D, ngakho 25 (OH) vd ubhekwa inkomba kangcono zokukala izinga Vitamin D.The Yokucwaninga Kit kusekelwe immunochromatography mase yalokho emizuzwini engu-15.

ISIMISO SOMTHETHO INQUBO
I ulwelwesi zedivayisi ekuhlolweni camera ne conjugate ka-BSA futhi 25 (OH) vd ku esifundeni test nembuzi unogwaja anti IgG omzimba ku esifundeni control. Umaka pad kuthiwa camera nge fluorescence uphawu anti 25 (OH) vd omzimba kanye unogwaja IgG kusengaphambili. Lapho ukuhlola isampula, 25 (OH) vd kwisempula okuhlangana ngayo fluorescence imakwe anti 25 (OH) vd omzimba, futhi yakha ingxube mzimba. Ngaphansi isenzo immunochromatography, ukugeleza eziyinkimbinkimbi bebheke iphepha umswakama, lapho eziyinkimbinkimbi lidlule esifundeni test, I khulula umaka Fluorescent uzobe kuhlanganiswe 25 (OH) vd phezu membrane.The lokuhlushwa 25 (OH) vd kuyinto ukuhlanganisa emibi isignali fluorescence, futhi lokuhlushwa 25 (OH) vd kwisempula kungatholakala by fluorescence immunoassay Assay.

Reagents nangezinto zokwakha

Izingxenye 25T iphakethe :
ikhadi .Test ngamunye ucwecwe pouched nge desiccant 25T
.A isixazululo 25T
.B isixazululo 1
.Package ufaka 1

TINTFO ADINGEKAYO KEPHA INIKEZELWA
Sample iqoqo esitsheni, timer

ISAMPULA IQOQO KANYE UKUGCINWA
amasampula 1.The ihlolwe kungaba serum, heparin anticoagulant oketshezini olungenambala olusegazini noma EDTA plasma anticoagulant.

2.According bavezelwe amathekniki ejwayelekile ukuqoqa isampula. Serum noma uketshezi olungenambala olusegazini isampula ingagcinwa esiqandisini ezingeni 2-8 ℃ ngoba 7days futhi cryopreservation ngezansi -15 ° C izinyanga ezingu-6.
3.All isampula Gwema nqabela-ncibilika imijikelezo.

Assay INQUBO
Inqubo yokuhlola ye insimbi bheka ibhuku lokusebenzisa immunoanalyzer. Inqubo reagent yokuhlola kanje

1.Lay eceleni zonke reagents futhi amasampuli ekamelweni lokushisa.
2.Open Ephathekayo Immune Analyser (Wiz-A101), faka ngemvume iphasiwedi ye-akhawunti ngokwendlela indlela ukusebenza kwe-ithuluzi, bese ufaka i-interface ukuthola.
3.Scan ikhodi dentification ukuqinisekisa into yokuhlola.
4.Take out ikhadi test kusukela isikhwama ucwecwe.
5.Insert ikhadi yokuhlola ku embobeni yekhadi, ihlole ikhodi QR, futhi ukucacisa nto test.
6.Add 30μL serum noma uketshezi olungenambala olusegazini isampula Isixazululo, uhlanganise kahle.
Isixazululo 7.Add 50μL B ingxube ngenhla, uhlanganise kahle.
8 .Shiya ingxube imizuzu engu-15.
9.Add 80μL ingxube lokuphuza kahle ekhadini.
10.Click inkinobho "ukuhlolwa ejwayelekile", ngemva kwemizuzu engu-10, lo mshini izothola ngokuzenzakalela ikhadi test, kungaba ukufunda Imiphumela iboniswe isihenqo insimbi, futhi urekhode / ukuphrinta imiphumela yokuhlolwa.
11.Refer imfundo enikezwa ephathekayo Immune Analyser (Wiz-A101).

IZIMO EZILINDELEKILE
25 (OH) vd uhla evamile: 30-100ng / ml

Kunconywa ukuthi laboratory ngamunye ukumisa okwabo siqu uhla yayo evamile emelela bakhona nesineke.

IMIPHUMELA YOKUHLOLWA KANYE UKUHUNYUSHWA
.I ngenhla idatha isithenjwa isikhawu olusungulelwe idatha ukuthola kule khithi, futhi wasikisela ukuthi laboratory ngamunye kumele ajube ithimba inkomba isikhawu ukubaluleka olufanele emtholampilo sabantu kule ndawo.

.I lokuhlushwa 25 (OH) vd ingaphezu kwe-inkomba ububanzi, futhi izinguquko bokuphila noma ukucindezeleka impendulo kufanele excluded.Indeed normal, kufanele sihlanganise emitholampilo uphawu uphethwe.
.I imiphumela le ndlela ziyasetshenziswa ububanzi inkomba zokuhlola ezasungulwa le ndlela kuphela, futhi imiphumela ngokuqondile ufana ezinye izindlela.
.Abanye izici ingabagulisa amaphutha ukuthola imiphumela, kuhlanganise Ngezizathu zezobuchwepheshe, amaphutha tekusebenta nezinye izinto isampula.

UKUGCINWA KANYE ESIZINZILE
.I kwekhithi izinyanga ezingu-18 eshalofini-ukuphila kusukela usuku ukwakhiwa. Gcina kits ubuningi at 2-30 ° C. Hhayi kwi freezer. Ungayisebenzisi ngale okungapheli.

.Ningavumeli ukuvula sikhwama uphawu kuze kube yilapho usukulungele ukwenza isivivinyo esithile, bese isivivinyo esisodwa-ukusebenzisa kusikiselwa ukuba kusetshenziswe ngaphansi imvelo edingekayo (lokushisa 2-35 ℃, umswakama 40-90%) ngaphakathi amaminithi 60 ngokukhulu ukushesha kungenzeka.
Diluent .Sample isetshenziswa ngokushesha ngemva kokuba kuvulwe.

IZIXWAYISO kanye Okumele kuqashelwe
.I ikhithi kufanele uphawu futhi uvikelekile umswakama.

.Konke zezinhlobo omuhle liyakushunyayelwa sigunyazwe nezinye izindlela.
Zezinhlobo .Konke ngeke ziphathwe njengemfucuza ukungcola ezingaba khona.
.INGABE usebenzise reagent yisikhathi.
.INGABE HHAYI Yokunikezelana reagents phakathi kits nge eziningi ezahlukene kungekho ..
.INGABE HHAYI usebenzise amakhadi test futhi iyiphi izesekeli zalutho.
.Misoperation, isampula ngokweqile noma kancane kungaholela umphumela ngeziphambeko.

L mbumbulu
.Njengoba nanoma yimuphi Assay kuqashwa igundane amasosha omzimba, kungenzeka ekhona ithonya lomuntu anti-igundane amasosha omzimba (Hama) e isifanekiso. Zezinhlobo ezigulini abamukele amalungiselelo omzimba monoclonal ngoba uphethwe noma ukwelashwa ingaqukatha Hama. Zezinhlobo ezinjalo zingakwenza Imiphumela okungamanga noma amanga ezimbi.

.Le yi ekuhlolweni kuphela inkomba yomtholampilo, akufanele laba yisisekelo kuphela uphethwe emitholampilo kanye nokwelashwa, iziguli ukuphathwa emitholampilo kufanele kube ukucabangela esibanzi kuhlangene izimpawu zayo, umlando wezempilo, nezinye luhlolo laboratory, impendulo ukwelashwa, lwezifo kanye neminye imininingwane .
.Le reagent isetshenziselwa kuphela ukuhlolwa serum ne-plasma. Kungase bathole yi olunembile uma isetshenziselwa ezinye amasampula ezifana ngamathe futhi umchamo futhi njll

UKUSEBENZA CHARACTERISTICS

Linearity 5 ng / ml ku-120 ng / ml ukuphambuka isihlobo: -15% kuya ku- + 15%.
Ukuthuthukiswa ukuhlanganisa Coefficient: (r) ≥0.9900
Ukunemba Izinga yokutakula uzakwenziwa phakathi 85% - 115%.
Repeatability CV≤15%
Ngokucacile
(Akekho izinto ngesikhathi interferent wavivinya liphazamisa ku Assay)
Interferent okuhlushwa Interferent
I-hemoglobin 200μg / ml
transferrin 100μg / ml
Horse isithombo peroxidase 2000μg / ml
Vitamin D3 50mg / ml
Vitamin D 50mg / ml

R EFERENCES

1.Hansen JH, et al.HAMA Impambaniso nge Immunoassays Murine Monoclonal omzimba-Isekelwe [J] .B ka Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.The Umhlobo Heterophilic Amasosha Omzimba kanye Indima e Immunoassay Impambaniso [J] .B ka Clin Immunoassay, 1992,15: 108-114.

Isihluthulelo izimpawu ezisetshenziswa:

 t11-1 Ngaphandle kwesibeletho Yokucwaninga Kudivayisi Medical
 tt-2 Umkhiqizi
 tt-71 Isitolo at 2-30 ℃
 tt-3 Usuku lokuphelelwa isikhathi
 tt-4 Ningabeki Sebenzisa
 tt-5 Ukuqapha
 tt-6 Bheka Imiyalelo Ukuze Sebenzisa

Xiamen Wiz Biotech CO, LTD.
Ikheli: 3-4 Floor, NO.16 Isakhiwo, Workshop Bio-ezokwelapha, wezi-2030 Wengjiao West Road, Haicang District, 361026, Xiamen, China
Ucingo: + 86-592-6808278
Ifeksi: + 86- 592-6808279


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