Ikhithi Yokuxilonga yeI-Calprotectin(Ukuhlolwa kwe-Fluorescence Immunochromatographic)
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga yeI-Calprotectin(I-Cal) ifanelekela ukunqunywa kwenani lendle yomuntu. I-Cal nge-fluorescence immunochromatographic assay, enenani elibalulekile lokuxilonga isifo samathumbu esivuvukalayo. Wonke amasampula abonisa ukuthi akhona kumele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Cal iyi-heterodimer, eyakhiwa yi-MRP 8 kanye ne-MRP 14^[1]Itholakala kuma-neutrophils cytoplasm futhi itholakala kuma-membranes eseli le-mononuclear. I-Cal iyi-acute phase proteins, ine-acute phase stage ehlala izinzile cishe isonto elilodwa endle yabantu, itholakala njengophawu lwesifo samathumbu esivuvukalayo.^[2-3]. Le khithi iyisivivinyo esilula, esibonakalayo esilinganisayo esithola i-Cal endle yabantu, sinokuzwela okuphezulu kokuthola kanye nokucaciswa okuqinile. Isivivinyo esisekelwe ekucacisweni okuphezulu kwama-antibodies amabili isimiso sokusabela kwe-sandwich kanye nobuchwepheshe bokuhlaziya i-fluorescence immunochromatographic assay, okunganikeza umphumela zingakapheli imizuzu eyi-15.

ISIMISO SENQUBO
I-strip ine-antibody enamathelayo ye-anti-Cal endaweni yokuhlola, eboshelwe ku-membrane chromatography kusengaphambili. I-Lable pad imbozwe yi-antibody enamathelayo ye-fluorescence kusengaphambili. Lapho kuhlolwa isampula enhle, i-Cal esampula ingaxutshwa ne-antibody enamathelayo ye-fluorescence, bese yakha ingxube ye-immune. Njengoba ingxube ivunyelwe ukuhamba eceleni kwe-test strip, i-Cal conjugate complex ibanjwa yi-antibody enamathelayo ye-anti-Cal ephepheni bese yakha i-complex. Ukuqina kwe-fluorescence kuhlobene kahle nokuqukethwe kwe-Cal. I-Cal esampula ingatholwa nge-fluorescence immunoassay analyzer.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：
Ikhadi lokuhlola lifakwe ifoyili ngalinye ngesikhwama se-desiccant esingu-25T
Amasampula okuhlanza angu-25T
Iphakheji yokufaka 1

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA
1. Sebenzisa isitsha esihlanzekile esilahlwayo ukuze uqoqe isampula yendle entsha, bese uhlolwa ngokushesha. Uma ingeke ihlolwe ngokushesha, sicela igcinwe ku-2-8°C izinsuku ezi-3 noma ngaphansi kuka-15°C izinyanga ezi-6.
2. Khipha induku yokusampula, efakwe kusampula yendle, phinda isenzo izikhathi ezi-3, thatha izingxenye ezahlukene zesampula yendle isikhathi ngasinye, bese ubuyisela induku yokusampula emuva, uyiqinise bese uyinyakazisa kahle, Noma usebenzisa induku yokusampula ethathe isampula yendle engama-50mg, bese uyifaka eshubhu lesampula yendle equkethe isampula yokuxubha, bese uyiqinisa.
3.Sebenzisa isampula yepayipi elilahlwayo thatha isampula yendle evela esigulini esinesifo sohudo, bese ufaka amaconsi amathathu (cishe u-100µL) epayipini lokuhlola indle bese ushukumisa kahle.

Amanothi:
1. Gwema imijikelezo yokuqandisa nokuncibilikisa.
2. Ncibilikisa amasampula abe semazingeni okushisa egumbi ngaphambi kokusebenzisa.

INQUBO YOKUHLOLA

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.
1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Susa isivalo epayipini lesampula bese ulahla amaconsi amabili okuqala esampula exutshwe, engeza amaconsi amathathu (cishe ama-uL ayi-100) isampula exutshwe ngamabhamuza iqonde phezulu bese kancane kancane ungene emthonjeni wesampula wekhadi nge-dispette enikeziwe.
7.Chofoza inkinobho ethi “ukuhlolwa okujwayelekile”, ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
8.Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

AMANANI ALINDELEKILE
Amakhalori <60μg/g
Kunconywa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumele iziguli zayo.

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA
1. I-Cal esampula ingaphezu kuka-60μg/g, futhi kufanele isuse ushintsho esimweni somzimba. Imiphumela ayijwayelekile ngempela futhi kufanele itholakale inezimpawu zomtholampilo.
2. Imiphumela yale ndlela isebenza kuphela kumabanga okubhekisela abekwe kule ndlela, futhi akukho ukuqhathaniswa okuqondile nezinye izindlela.
3. Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA
1. Ikhithi ihlala izinyanga ezingu-18 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
3. Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
UNGASEBENZISI i-reagent ephelelwe yisikhathi.
UNGASHINTSHI ama-reagent phakathi kwamakhithi anenombolo ehlukile.
UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

UMKHAWULO
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zaso, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwendle. Ingase ingatholi umphumela onembile uma isetshenziswa kwezinye izibonelo ezifana namathe nomchamo njll.

IZIMALI ZOKUSEBENZA

IZINKOMBA
1.Li,G.&Y.L.Li.Ubudlelwano phakathi kwe-calcium nesifo somtholampilo[J].I-Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,etc. Ucwaningo lwezokwelapha lwe-calamine ne-lactoferrin ezigulini ezine-ulcerative colitis [J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,etc. Ucwaningo mayelana nobudlelwano phakathi kwe-calamine yendle nesifo samathumbu esivuvukalayo[J]. Isayensi Yezobuchwepheshe Nobunjiniyela,2010-03,10(8)

Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa

I-Xiamen Wiz Biotech CO.,LTD
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