Ikhithi Yokuxilonga ye-microalbumin yomchamo

(Ukuhlolwa kwe-Fluorescence Immunochromatographic)

Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela

Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga ye-Urine microalbumin (i-Fluorescence Immunochromatographic Assay) ifaneleka ekutholakaleni kwenani le-microalbumin emchameni womuntu nge-fluorescence immunochromatographic assay, esetshenziswa kakhulu ekuxilongweni okusizayo kwesifo sezinso. Zonke izibonelo ezinhle kumele ziqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Microalbumin iyiprotheni evamile etholakala egazini futhi ayivamile kakhulu emchameni uma iguqulwa ibe yi-metabolism evamile. Uma kukhona inani elincane emchameni, i-Albumin ingaphezu kwama-micron/mL angama-20, ingeye-microalbumin yomchamo, uma kungenzeka ukwelashwa ngesikhathi esifanele, ingalungisa ngokuphelele i-glomeruli, iqede i-proteinuria, uma kungenjalo ukwelashwa ngesikhathi esifanele, ingangena esigabeni se-uremia. Ukwanda kwe-microalbumin yomchamo kubonakala kakhulu kwi-nephropathy yesifo sikashukela, umfutho wegazi ophakeme kanye ne-preeclampsia ekukhulelweni. Lesi simo singahlolwa ngokunembile ngenani le-microalbumin yomchamo, kuhlanganiswe nokwanda kwayo, izimpawu kanye nomlando wezokwelapha. Ukutholwa kusenesikhathi kwe-microalbumin yomchamo kubaluleke kakhulu ukuvimbela nokubambezela ukuthuthukiswa kwe-nephropathy yesifo sikashukela.

ISIMISO SENQUBO

I-membrane yedivayisi yokuhlola imbozwe nge-ALB antigen endaweni yokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG endaweni yokulawula. Iphedi yomaka imbozwe nge-antibody ye-ALB uphawu lwe-fluorescence kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-ALB kusampula ihlangana ne-antibody ye-ALB uphawu lwe-fluorescence, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, i-free fluorescent marker izohlanganiswa ne-ALB ku-membrane. Ukuhlushwa kwe-ALB kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-ALB kusampula kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：

Ikhadi lokuhlola lifakwe ifoyili ngalinye ngesikhwama se-desiccant esingu-25T

Iphakheji yokufaka 1

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA

Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA

1. Amasampula ahlolwe angaba umchamo.
2. Amasampula omchamo amasha angaqoqwa esitsheni esihlanzekile esilahlwayo. Kunconywa ukuhlola amasampula omchamo ngokushesha ngemva kokuqoqwa. Uma amasampula omchamo engakwazi ukuhlolwa ngokushesha, sicela uwagcine ku-2-8℃, kodwa kunconywa ukuthi ungagciniZibeke isikhathi esingaphezu kwamahora ayi-12. Ungawushukumisi umgqomo. Uma kukhona indle phansi kwesitsha, thatha i-supernatant ukuze uyihlole.
3. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.
4. Ncibilikisa amasampula abe yizinga lokushisa legumbi ngaphambi kokusebenzisa.