Ikhithi Yokuxilonga(I-1965)Igolide le-Colloidal）yeCalprotectin
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga yeCalprotectin (cal) iyisivivinyo se-colloidal gold immunochromatographic sokunquma i-calcium ephuma endle yabantu, enenani elibalulekile lokuxilonga isifo samathumbu esivuvukalayo. Lokhu kuhlolwa kuyi-reagent yokuhlola. Wonke amasampula abonisa ukuthi akhona kumele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela. Okwamanje, lokhu kuhlolwa kusetshenziselwa i-IVD, amathuluzi engeziwe awadingeki.

ISIFINYEZO
I-Cal iyi-heterodimer, eyakhiwa yi-MRP 8 kanye ne-MRP 14. Itholakala kuma-neutrophils cytoplasm futhi itholakala kuma-membranes eseli le-mononuclear. I-Cal iyi-acute phase proteins, ine-acute phase ezinzile cishe isonto elilodwa endle yabantu, itholakala njengophawu lwesifo samathumbu esivuvukalayo. Ikhithi iyisivivinyo esilula, esibonakalayo se-semiqualitative esithola i-cal endle yabantu, inokuzwela okuphezulu kokuthola kanye nokucaciswa okuqinile. Isivivinyo esisekelwe ku-high specificit double antibodies sandwich reaction principle kanye ne-gold immunochromatographic assay analysis techniques, singanikeza umphumela zingakapheli imizuzu eyi-15.

ISIMISO SENQUBO
I-strip ine-anti-cal coating McAb endaweni yokuhlola kanye ne-anti-rabbit IgG antibody yembuzi endaweni yokulawula, eboshelwe ku-membrane chromatography kusengaphambili. I-lable pad imbozwe nge-colloidal gold ebhalwe nge-anti-cal McAb kanye ne-colloidal gold ebhalwe nge-rabbit IgG antibody kusengaphambili. Lapho kuhlolwa isampula enhle, i-cal esampula ihlanganiswe ne-colloidal gold ebhalwe nge-anti-cal McAb, futhi yakha i-immune complex, njengoba ivunyelwe ukuhamba eceleni kwe-test strip, i-cal conjugate complex ibanjwa yi-anti-cal coating McAb e-membrane bese yakha i-“anti-cal coating McAb-cal-colloidal gold ebhalwe nge-anti-cal McAb” complex, ibhendi yokuhlola enemibala yavela endaweni yokuhlola. Ubukhulu bombala buhlobene kahle nokuqukethwe kwe-cal. Isampula engemihle ayikhiqizi ibhendi yokuhlola ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Kungakhathaliseki ukuthi i-cal ikhona kusampula noma cha, kukhona umugqa obomvu ovela esifundeni sokubhekisela kanye nesifunda sokulawula ikhwalithi, okubhekwa njengezindinganiso zebhizinisi zangaphakathi zekhwalithi.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE
Izingxenye zephakheji ezingama-25T：
Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant
.Izibonelo zokuxuba: izithako zingama-20mM pH7.4PBS
.Dispette
.Iphakheji yokufaka

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA

Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA
Sebenzisa isitsha esihlanzekile esilahlwayo ukuze uqoqe isampula yendle entsha, bese uhlolwa ngokushesha. Uma ingeke ihlolwe ngokushesha, sicela uyigcine ku-2-8°C amahora ayi-12 noma ngaphansi kuka-15°C izinyanga ezi-4.

INQUBO YOKUHLOLA
1. Khipha induku yokusampula, efakwe kusampula yendle, bese ubuyisela induku yokusampula, uyiqinise bese uyinyakazisa kahle, uphinde isenzo izikhathi ezintathu. Noma usebenzisa induku yokusampula ekhethwe cishe isampula yendle engu-50mg, bese uyifaka eshubhu lesampula yendle equkethe isampula yokuxubha, bese uyiqinisa.
2.Sebenzisa isampula yepayipi elilahlwayo thatha isampula yendle encane evela esigulini esinesifo sohudo, bese ufaka amaconsi amathathu (cishe ama-uL ayi-100) epayipini lokuhlola indle bese ushukumisa kahle, ubeke eceleni.
3. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
4. Susa isivalo epayipini lesampula bese ulahla amaconsi amabili okuqala esampula exutshwe, engeza amaconsi amathathu (cishe ama-uL ayi-100) isampula exutshwe ngamabhamuza iqonde phezulu bese kancane kancane ungene emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.
5. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA

ISITOKELO NOKUZINZIMA
Ikhithi ihlala izinyanga ezingama-24 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. Ungavuli isikhwama esivaliwe kuze kube yilapho usukulungele ukwenza isivivinyo.
IZIXWAYISO NEZIQAPHELO ZOKWENZA
1. Ikhithi kufanele ivalwe futhi ivikelwe emswakama¹.
2. Ungasebenzisi isampula ebekwe isikhathi eside kakhulu noma eqandisiwe futhi incibilikiswa ngokuphindaphindiwe ukuze uyihlole
3. Amasampula endle agcwele ngokweqile noma ukujiya kungenza amasampula axutshwe abe yikhadi lokuhlola elingcolile, sicela ufake isampula exutshwe bese uthatha i-supernatant ukuze iyohlolwa.
4. Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

UMKHAWULO
1. Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zalo, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.².
2. Le reagent isetshenziswa kuphela ekuhlolweni kwendle. Ingase ingatholi umphumela onembile uma isetshenziswa kwezinye izibonelo ezifana namathe nomchamo njll.