Isingeniso: Ukubaluleka Komtholampilo Kokuqapha Komsebenzi Wezinso Wakuqala:

Isifo sezinso esingamahlalakhona (CKD) sesiphenduke inselele yezempilo yomphakathi emhlabeni jikelele. Ngokwezibalo zeNhlangano Yezempilo Yomhlaba, cishe abantu abayizigidi ezingu-850 emhlabeni wonke bahlushwa izifo ezihlukahlukene zezinso, kanti ukwanda kwezifo zezinso ezingamahlalakhona emhlabeni wonke kulinganiselwa ku-9.1%. Okubi nakakhulu ukuthi isifo sezinso esingapheli ngokuvamile asinazo izimpawu ezicacile, okwenza inani elikhulu leziguli liphuthelwe isikhathi esingcono kakhulu sokungenelela. Ngokumelene nalesi sizinda,I-microalbuminuria, njengenkomba ebucayi yokulimala kwezinso zakuqala, iye yaba yigugu kakhulu. Izindlela zendabuko zokuhlola ukusebenza kwezinso ezifana ne-serum creatinine kanye nesilinganiso sokuhlunga kwe-glomerular (eGFR) sizobonisa kuphela okungavamile lapho ukusebenza kwezinso kulahleka ngaphezu kuka-50%, kuyilapho ukuhlolwa kwe-albumin yomchamo kunganikeza amasignali ayisixwayiso kusenesikhathi lapho ukusebenza kwezinso kulahleka ngo-10-15%.

Inani lomtholampilo kanye nesimo samanje seI-ALBukuhlolwa komchamo

I-albhamuin (ALB) iyiphrotheni eningi kakhulu emchameni wabantu abanempilo, enezinga elivamile lokuphuma elingaphansi kuka-30mg/24h. Uma izinga lokuphuma kwe-albumin yomchamo lingaphakathi kwebanga elingu-30-300mg/24h, lichazwa ngokuthi i-microalbuminuria, futhi lesi sigaba yisikhathi esihle kakhulu sokungenelela ukuze kubuyiselwe umonakalo wezinso. Njengamanje, okuvame ukusetshenziswaI-ALBIzindlela zokutholwa emisebenzini yomtholampilo zihlanganisa i-radioimmunoassay, i-enzyme-linked immunosorbent assay (ELISA), i-immunoturbidimetry, njll., kodwa lezi zindlela ngokuvamile zinezinkinga ezifana nokusebenza okuyinkimbinkimbi, ukusetshenziswa isikhathi eside, noma isidingo semishini ekhethekile. Ikakhulukazi ezikhungweni zokwelapha eziyinhloko kanye nezimo zokuqapha ekhaya, ubuchwepheshe obukhona bunzima ukuhlangabezana nezidingo ezilula, isivinini, nokunemba, okuholela ekutheni inani elikhulu leziguli ezinokulimala kwezinso zakuqala lingatholakali ngesikhathi.

Ukuphumelela Okusha ku-PrecisionUkuhlolwa komchamo kwe-ALBI-Reagent

Ukuphendula imikhawulo yobuchwepheshe bokuhlola obukhona, inkampani yethu ithuthukise i-PrecisionUkuhlolwa komchamo kwe-ALB I-Reagent yokuthola impumelelo eminingi kwezobuchwepheshe. I-reagent isebenzisa ubuchwepheshe obuthuthukisiwe be-immunochromatographic obuhlobene kakhulu kanye nokucaciswa okuphezulu kwe-albumin ye-albumin ye-monoclonal yomuntu ukuze kuqinisekiswe ukunemba nokuthembeka kokuhlolwa. Ukuqamba okusha kwezobuchwepheshe kubonakala kakhulu ezicini ezintathu:

• Ukuzwela okuthuthuke kakhulu: umkhawulo ophansi wokutholwa ufinyelela ku-2mg/L, futhi ukwazi ukubona ngokunembile umkhawulo womchamo we-microalbumin ongu-30mg/24h, ongcono kakhulu kunokuzwela kwemicu yokuhlola evamile.
• Ikhono elithuthukisiwe lokulwa nokuphazamiseka: Ngokuklama kwesistimu ye-buffer eyingqayizivele, inganqoba ngempumelelo ukuphazamiseka kokuguquguquka kwe-pH yomchamo, izinguquko zamandla e-ionic nezinye izici emiphumeleni yokuhlola, iqinisekisa ukuzinza kokuhlolwa ngaphansi kwezimo ezihlukahlukene zomzimba.
• Ukutholwa komthamo okusha: umfundi oyisipesheli osekelayo angakwazi ukubona isilinganiso esincane ukuya ekutholweni komthamo, uhla lokutholwa luhlanganisa u-0-200mg/L, ukuze kuhlangatshezwane nezidingo zomtholampilo ezihlukene kusukela ekuhlolweni kuye ekuqashweni.

Ukusebenza Komkhiqizo kanye Nezinzuzo

Iqinisekiswe ngokomtholampilo ezibhedlela eziningana zemfundo ephakeme, lesi siphehli sikhombisa izinkomba zokusebenza ezinhle kakhulu. Uma kuqhathaniswa nesilinganiso segolide esijwayelekile somchamo wamahora angu-24, i-coefficient yokuhlanganisa ifinyelela ngaphezu kuka-0.98; ama-coefficients angaphakathi kanye ne-inter-batch okuhlukahluka angaphansi kuka-5%, aphansi kakhulu kunezinga lemboni; isikhathi sokutholwa siyimizuzu eyi-15 kuphela, okuthuthukisa kakhulu ukusebenza kahle komtholampilo. Izinzuzo zomkhiqizo zifingqiwe ngezansi:

• Ukusebenza okulula: asikho isidingo sokwelashwa kwangaphambili okuyinkimbinkimbi, amasampula omchamo angaba ngqo kusampula, ukuhlinzwa okuzinyathelo ezintathu ukuqedela ukuhlolwa, abangewona ochwepheshe bangakwazi kahle ngemva kokuqeqeshwa okufushane.
• Imiphumela enembile: ukusetshenziswa kwesistimu yokuthuthukisa umbala ocacile, iso lenyama lingafundwa ekuqaleni, amakhadi ombala afanayo angaba ukuhlaziywa okulinganiselwe, ukuhlangabezana nezidingo zezimo zohlelo lokusebenza ezahlukene.
• Umnotho futhi uyasebenza: izindleko zokuhlolwa okukodwa ziphansi kakhulu kunezokuhlolwa kwaselabhorethri, ezifanele ukuhlolwa okukhulu nokuqapha isikhathi eside, futhi kunenani elivelele lezomnotho lezempilo.

• Inani lesexwayiso sangaphambi kwesikhathi: ukulimala kwezinso kungatholwa eminyakeni engu-3-5 ngaphambili kunezinkomba zendabuko zokusebenza kwezinso, kuzuze isikhathi esibalulekile sokungenelela emtholampilo.

Izimo zesicelo somtholampilo kanye nezincomo zomhlahlandlela

UkunembaI-ALB Urine Testinezinhlobonhlobo zezimo zohlelo lokusebenza. Emkhakheni wesifo sikashukela i-mellitus, imihlahlandlela ye-American Diabetes Association (ADA) incoma ngokusobala ukuthi zonke iziguli ezinesifo sikashukela sohlobo loku-1 ≥ iminyaka emi-5 nazo zonke iziguli ezinesifo sikashukela sohlobo 2 kufanele zihlolwe umchamo we-albin njalo ngonyaka. Ekulawulweni kwe-hypertension, imihlahlandlela ye-ESC/ESH yomfutho wegazi ophezulu ibala i-microalbuminuria njengophawu olubalulekile lokulimala kwesitho esiqondiwe. Ngaphezu kwalokho, i-reagent ifanele izimo eziningi ezifana nokuhlola ingozi yesifo senhliziyo, ukuhlolwa kokusebenza kwezinso kubantu asebekhulile, nokuqapha kwezinso ngesikhathi sokukhulelwa.

Okuthakazelisa kakhulu ukuthi lo mkhiqizo ufanelana ngokuphelele nezidingo zokuxilongwa nokwelashwa kwe-hierarchical. Ingasetshenziswa njengethuluzi elisebenzayo lokuhlola isifo sezinso ezikhungweni zokwelapha eziyisisekelo njengezibhedlela zomphakathi kanye nezikhungo zezempilo zasemalokishini; eminyangweni ye-nephrology kanye ne-endocrinology yezibhedlela ezijwayelekile, ingasetshenziswa njengethuluzi elibalulekile lokulawulwa kwezifo kanye nokuqapha ukusebenza kahle; ezikhungweni zokuhlolwa kwezokwelapha, ingafakwa kumaphakheji okuhlola impilo ukuze kwandiswe izinga lokutholwa kokulimala kwezinso kusenesikhathi; futhi kulindeleke ukuthi ingene emakethe yokuqapha impilo yomndeni ngemuva kokuqinisekiswa okwengeziwe esikhathini esizayo.

Isiphetho

I-We Baysen Medical ihlale igxile kumasu okuxilonga ukuze sithuthukise izinga lempilo. Senze izinkundla zobuchwepheshe ezi-5- i-Latex, igolide eli-colloidal, i-Fluorescence Immunochromatographic Assay, i-Molecular,Chemiluminescence Immunoassay.SinayoUkuhlolwa kwe-ALB FIA Ukuqapha ukulimala kwezinso kwesigaba sokuqala