Ukuhlolwa Kwasekuqaleni, Ukuthula Kwengqondo: Ukuqonda i-HCV kanye Nobuchwepheshe Obubili Obukhulu Bokuthola Okusheshayo

Igciwane le-Hepatitis C (i-HCV) Ukutheleleka kuyinkinga yezempilo yomphakathi yomhlaba wonke. Kudluliselwa kakhulu ngegazi, futhi ukutheleleka kwasekuqaleni kuvame ukuba nezimpawu ezingenazimpawu, okushiya abantu abaningi abangenwe yileli gciwane bengazi ngesimo sabo. Kodwa-ke, leli gciwane lilimaza isibindi buthule, okungenzeka lithuthukele ekubeni yi-fibrosis yesibindi, i-cirrhosis, ngisho nomdlavuza wesibindi. Ngakho-ke, ukuhlolwa nokuxilongwa kwasekuqaleni kuyizinyathelo zokuqala ezibalulekile ekumiseni ukuqhubeka kwesifo kanye nokuthuthukisa amazinga okuphulukiswa.

Okwamanje, okokuqalaI-HCV ukuhlolwa kuncike kakhulu ekutholeni ukuba khonaI-HCVama-antibodies egazini. Ngokuthuthuka kwezobuchwepheshe, izindlela zokuhlola ezilula nezisheshayo zenze kwaba lula kakhulu ukuhlola, phakathi kwazo ukuhlolwa kwegolide kwe-colloidal kanye nama-reagent e-fluorescent immunoassay okuyizindlela ezimbili zobuchwepheshe ezisetshenziswa kabanzi.

Ukuhlolwa Okusheshayo Kwegolide Le-Colloidal: “I-Scout” Esheshayo Nelula

Indlela yegolide le-colloidal ubuchwepheshe obuvame ukusetshenziswa kulokho esikubiza ngokuthi “ukuhlolwa okusheshayo.” Isimiso sayo esiyinhloko sihilela ukusebenzisa ama-nanoparticle egolide alungiselelwe ngokukhethekile njengama-tracer.I-HCV Ama-antibodies akhona kusampula, abopheka ngqo kuma-antigen aklanywe kusengaphambili ku-reagent futhi aqongelela emgqeni wokuhlola, okwenza izinhlayiya zegolide ezingabonakali ekuqaleni zihlangane futhi zakhe ibhande elibomvu elicacile.

Izinzuzo ezinkulu zale ndlela yilezi:

1) Isivinini:Isivinini ngokuvamile singahlolwa ngokubona ngaphakathi kwemizuzu eyi-15-20.

2) Kulula ukusebenza:Akukho mishini eyinkimbinkimbi edingekayo; abasebenzi abaqeqeshwe bangaqedela inqubo ngisho nasezikhungweni zokunakekelwa kwempilo eziyisisekelo noma ezindaweni ezithile zokufakwa.

3) Ukuqina okuhle:Ama-reagent ngokuvamile angagcinwa futhi athuthwe ekushiseni kwegumbi.

Ngakho-ke, ukuhlolwa okusheshayo kwe-colloidal gold kufaneleka kahle ekuhlolweni kokuqala okukhulu, ukuhlolwa okuphuthumayo, kanye nokuhlolwa kokuqala ezindaweni ezinezinsizakusebenza zezokwelapha ezilinganiselwe, okuhlonza ngempumelelo abantu abangaba negciwane kubantu.

I-Fluorescent Immunoassay Diagnostic Reagent: “Umhlaziyi” Ozwelayo Nonembile

Ukuhlolwa kwe-fluorescent immunochromatographic kumelela ukuthuthukiswa okuphawulekayo kubuchwepheshe bendabuko be-chromatography. Kusebenzisa ama-microsphere akhethekile e-fluorescent njengezimpawu. Ngemva kokuba kwenzeke ukusabela kwe-antigen-antibody, izinto ze-fluorescent eziqoqwe emgqeni wokuhlola zikhipha isignali ebonakalayo ye-fluorescent uma zivuselelwa umthombo othile wokukhanya.

Uma kuqhathaniswa nendlela yegolide ye-colloidal, izici zayo eziyinhloko yilezi:

1) Isaziso Esiphezulu kanye Nekhwalithi Ye-Waveform:Iyakwazi ukubona ukumelana okugxilile, okuholela emiphumeleni enembile kakhulu futhi inciphise ngempumelelo ingozi yokutholwa okungafanele kanye nemiphumela engamanga.

2) Ukuhlaziywa Kwenani:Ukufunda ukuqina kwe-fluorescence usebenzisa i-fluorescence detector ezinikele kunikeza ulwazi olucebile lokuthola.

3) Okufanele Kakhulu Ukuzenzakalela:Ihlangana kalula nemishini yokuthola, ithuthukisa ukusebenza kahle kokuhlolwa kweqembu emalabhorethri.

Ama-reagents e-fluorescent immunoassay asetshenziswa kakhulu ezibhedlela, eminyangweni yelabhorethri, ezikhungweni zokulawula izifo, noma emalabhorethri okuhlola avela eceleni. Asebenza njengokuqinisekiswa okwengeziwe kwamasampula abonisa ukuthi akhona noma asetshenziswa ngqo ezimweni zokuhlolwa kwemitholampilo ezidinga ukunemba okuphezulu.

Isifinyezo

Kungakhathaliseki ukuthi ukuhlolwa kwegolide kwe-colloidal, ithuluzi elinamandla lokuhlola okusheshayo kokuqala, noma ukuhlolwa kwe-fluorescent immunoasses, ukuqinisekiswa okunembile kwendlela ekhethwayo, kokubili kungamathuluzi abalulekile ekulweni ne-hepatitis C. Ukukhetha indlela kuncike esimweni esithile sokusetshenziswa, izinsiza ezitholakalayo, kanye nezidingo zokuhlola.

I-Baysen Medical ihlala igxile ebuchwephesheni bokuxilonga ukuze ithuthukise ikhwalithi yempilo. Sithuthukise amapulatifomu obuchwepheshe ayi-5 - i-Latex, igolide le-colloidal, i-Fluorescence Immunochromatographic Assay, i-Molecular, i-Chemiluminescence Immunoassay, i-OurUkuhlolwa Okusheshayo kwe-HCVFuthiI-reagent ye-HCV FIA kulula ukusebenza futhi ungathola umphumela wokuhlolwa ngemizuzu eyi-15