KA'IDA

An shafa membrane na na'urar gwajin da maganin rigakafi na Rotavirus Group A a yankin gwaji da kuma maganin rigakafi na IgG na maganin akuya a yankin sarrafawa. Ana shafa murfin label da hasken haske mai lakabin anti Rotavirus Group A da kuma maganin IgG na zomo a gaba. Lokacin gwada samfurin da ya dace, RV a cikin samfurin yana haɗuwa da hasken haske mai lakabin anti Rotavirus Group A, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun zuwa ga takardar sha. Lokacin da hadaddun ya wuce yankin gwaji, ya haɗu da maganin rigakafi na Rukunin A mai rufi na anti-Rotavirus, yana samar da sabon hadadden tsari. Idan ya kasance mara kyau, babu maganin rigakafi na Rotavirus Group A a cikin samfurin, don haka ba za a iya samar da hadaddun garkuwar jiki ba, ba za a sami layin ja a yankin ganowa ba (T). Ko rotavirus na rukuni na A yana cikin samfurin, ana sanya IgG na linzamin kwamfuta mai lakabin latex zuwa yankin sarrafa inganci (C) kuma an kama shi da maganin rigakafi na IgG na akuya. Layin ja zai bayyana a yankin sarrafa inganci (C). Layin ja shine ma'aunin da ke bayyana a yankin kula da inganci (C) don tantance ko akwai isassun samfura da kuma ko tsarin chromatography na al'ada ne. Haka kuma ana amfani da shi azaman ma'aunin kula da ciki don reagents.

Tsarin Gwaji:

1. Ya kamata a tattara marasa lafiya da ke da alamun cutar. A cewar rahotanni, mafi girman fitar rotavirus a cikin najasar marasa lafiya da ke fama da cutar gastroenteritis yana faruwa ne kwanaki 3-5 bayan fara cutar da kuma kwanaki 3-13 bayan fara alamun cutar. Idan an ɗauki samfurin tsawon lokaci bayan gudawa, adadin antigens ɗin bazai isa ya haifar da sakamako mai kyau ba.

2. Ya kamata a tattara samfuran a cikin akwati mai tsabta, busasshe, mai hana ruwa shiga wanda ba ya ƙunshe da sabulun wanki da abubuwan kiyayewa.

3. Ga marasa lafiya da ba sa fama da gudawa, samfurin najasar da aka tattara bai kamata ya zama ƙasa da gram 1-2 ba. Ga marasa lafiya da gudawa, idan najasar ruwa ce, da fatan za a tattara aƙalla 1-2 ml na ruwan najasar. Idan najasar ta ƙunshi jini da yawa da majina, da fatan za a sake tattara samfurin.

4. Ana ba da shawarar a gwada samfuran nan da nan bayan an tattara su, in ba haka ba, a aika su zuwa dakin gwaje-gwaje cikin awanni 6 a adana su a zafin 2-8°C. Idan ba a gwada samfuran ba cikin awanni 72, ya kamata a adana su a zafin da ke ƙasa da -15°C.

5. Yi amfani da najasa sabo don gwaji, sannan a haɗa samfuran najasa da ruwan da aka tace ko aka tace