KA'IDA

An shafa membrane na na'urar gwajin da maganin rigakafi na FOB a yankin gwaji da kuma maganin rigakafi na IgG na akuya a yankin sarrafawa. Ana shafa murfin label da sinadarin fluorescence mai suna anti FOB antibody da kuma maganin IgG na zomo a gaba. Lokacin gwada samfurin tabbatacce, maganin rigakafi na FOB a cikin samfurin yana haɗuwa da maganin rigakafi mai suna fluorescence mai suna anti FOB antibody, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun zuwa ga takardar sha. Lokacin da hadaddun ya wuce yankin gwaji, an haɗa shi da maganin rigakafi mai suna anti FOB, yana samar da sabon hadaddun. Idan ya kasance mara kyau, samfurin bai ƙunshi hemoglobin na ɗan adam ba ko kuma abun da ke ciki ya yi ƙasa, ba za a iya samar da isasshen hadaddun garkuwar jiki ba. Ba za a sami layin ja a yankin ganowa (T) ba. Layin ja shine ma'aunin yana bayyana a yankin sarrafa inganci (C) don tantance ko akwai isassun samfura da kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani da shi azaman ma'aunin sarrafawa na ciki don reagents.

Tsarin Gwaji:

Da fatan za a karanta abin da aka saka a cikin fakitin kafin a gwada.

1. Cire katin gwaji daga jakar foil, sanya shi a kan teburin matakin sannan ka yi masa alama.

2. Cire murfin daga bututun samfurin kuma jefar da digo biyu na farko na samfurin da aka narkar, ƙara digo 3 (kimanin 100uL) ba tare da kumfa ba a tsaye da hankali a cikin rijiyar samfurin na katin tare da dispette da aka bayar. Sannan kunna mai ƙidayar lokaci.

3. Ya kamata a karanta sakamakon cikin mintuna 10-15, kuma ba shi da inganci bayan mintuna 15.