Kit ɗin bincike donLuteinizing Hormonefluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai.Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin Diagnostic don Luteinizing Hormone (ƙaddamar da fluorescence immunochromatographic assay) shine ƙimar immunochromatographic mai haske don gano ƙididdigar ƙima na Luteinizing Hormone (LH) a cikin ƙwayar ɗan adam ko plasma, wanda galibi ana amfani dashi a cikin kimanta aikin endocrin pituitary. Duk samfurin tabbatacce dole ne a tabbatar da shi ta hanyar. sauran hanyoyin.Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.

TAKAITACCEN

Luteinizing hormone (LH) wani glycoprotein ne mai nauyin kwayoyin halitta na kimanin 30,000 Dalton, wanda aka samar da pituitary na baya.Matsakaicin LH yana da alaƙa da kusancin ovulation na ovaries, kuma ana hasashen kololuwar LH zai kasance awanni 24 zuwa 36 na ovulation.Sabili da haka, ana iya lura da ƙimar mafi girma na LH a lokacin hawan haila don ƙayyade mafi kyawun lokacin daukar ciki.Ayyukan endocrin mara kyau a cikin glandon pituitary zai iya haifar da rashin daidaituwa na LH. Ana iya amfani da ƙaddamar da LH don kimanta aikin endocrin pituitary.Kit ɗin bincike ya dogara ne akan immunochromatography kuma yana iya ba da sakamako cikin mintuna 15.

KA'IDAR HANYA

Ana lulluɓe jikin na'urar gwajin da anti-LH antibody akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa.Label pad an lullube shi ta hanyar walƙiya mai lakabin anti LH antibody da zomo IgG a gaba.Lokacin gwada ingantaccen samfurin, antigen na LH a cikin samfurin yana haɗuwa tare da walƙiya mai lakabin anti LH antibody, kuma suna samar da cakuda rigakafi.A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, shi hade da anti LH shafi antibody, Forms sabon hadaddun.Matsayin LH yana da alaƙa da alaƙa da siginar kyalli, kuma ana iya gano maida hankali na LH a cikin samfur ta hanyar gwajin immunoassay fluorescence.

REAgents DA KAYAN DA AKA BAYAR

Abubuwan kunshin 25T:

.Katin gwajin daidaiku da foil ɗin da aka shayar da shi tare da 25T na busa
.Sample diluents
. Kunshin saka

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabarun tattara samfurin.Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
3.All samfurin guje wa daskare-narke hawan keke.

HANYAR ASSAY

Hanyar gwaji na kayan aiki duba jagorar immunoanalyzer.Hanyar gwajin reagent shine kamar haka
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6.Add 20μL serum ko plasma samfurin zuwa samfurin diluent, da kuma Mix da kyau ..
7.Add 80μL samfurin bayani don samfurin rijiyar katin.
8. Danna maɓallin "gwajin misali", bayan minti 15, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
9. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).

SAKAMAKON JARRABAWA DA FASSARA

.Bayanan da ke sama shine tazara tazara da aka kafa don gano bayanan wannan kit ɗin, kuma ana ba da shawarar cewa kowane ɗakin gwaje-gwaje ya kamata ya kafa tazara tazara don mahimmancin asibiti da ya dace na yawan jama'a a wannan yanki.
.Ƙaƙƙarwar LH ya fi girma fiye da ma'anar tunani, kuma ya kamata a cire sauye-sauyen ilimin lissafi ko amsawar damuwa.Hakika maras kyau, ya kamata ya haɗu da ganewar asali na asibiti.
.Sakamakon wannan hanyar yana aiki ne kawai ga kewayon tunani da aka kafa ta wannan hanyar, kuma sakamakon ba ya daidaita kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.

AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi.Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C.KAR KA DANKE.Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.

GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.BA musanya reagents tsakanin kits tare da daban-daban kuri'a No..
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.

LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin.Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA.Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, kula da lafiyar marasa lafiya ya kamata a yi la'akari da shi sosai tare da bayyanar cututtuka, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi kawai don gwajin jini da jini.Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.

HALAYEN YI

NASARA
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.

Maɓalli ga alamomin da aka yi amfani da su:

	In Vitro Diagnostic Medical Na'urar
	Mai ƙira
	Adana a 2-30 ℃
	Ranar Karewa
	Kada a sake amfani
	HANKALI
	Tuntuɓi Umarnin Don Amfani

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