Ikhithi yokuxilonga yeI-Luteinizing Hormonei-fluorescence immunochromatographic assay)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo.Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuhlola I-Luteinizing Hormone (i-fluorescence immunochromatographic assay) iwukuhlola kwe-fluorescence immunochromatographic ukuze kutholwe inani le-Luteinizing Hormone (LH) ku-serum yomuntu noma ku-plasma, esetshenziswa kakhulu ekuhlolweni komsebenzi we-pituitary endocrine. ezinye izindlela.Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Luteinizing hormone (LH) iyi-glycoprotein enesisindo samangqamuzana esingaba ngu-30,000 Dalton, ekhiqizwa i-anterior pituitary.Ukuhlushwa kwe-LH kuhlobene eduze nokuvuthwa kwama-ovari, futhi ukuphakama kwe-LH kubikezelwa ukuthi kungaba amahora angu-24 kuya kwangu-36 ovulation.Ngakho-ke, inani eliphakeme le-LH lingagadwa phakathi nomjikelezo wokuya esikhathini ukuze kunqunywe isikhathi esifanelekile sokukhulelwa.Ukusebenza okungavamile kwe-endocrine ku-pituitary gland kungabangela i-LH secretion irregularity.I-concentration ye-LH ingasetshenziswa ukuhlola ukusebenza kwe-pituitary endocrine.Ikhithi Yokuxilonga isekelwe ku-immunochromatography futhi inganikeza umphumela phakathi nemizuzu eyi-15.

ISIMISO SENKQUBO

I-membrane yedivayisi yokuhlola imbozwe nge-anti-LH antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula.Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi anti-LH antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-antigen ye-LH kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti-LH antibody, bese yenza ingxube yamasosha omzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti LH yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Izinga le-LH lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-LH kusampula kungatholwa nge-fluorescence immunoassay assay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:

.Iphepha lokuhlola ngalinye elifakwe esikhwameni nge-desiccant 25T
.Izihlanjululi zesampula
.Faka iphakheji

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isitsha seqoqo lesampula, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Amasampula ahloliwe angaba i-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2.Ngokwezinqubo ezijwayelekile qoqa isampula.I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-7 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-6.
3.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.

INQUBO YOKUHLOLA

Inqubo yokuhlola yensimbi bheka imanuwali ye-immunoanalyzer.Inqubo yokuhlola i-reagent imi kanje
1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4.Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6.Engeza i-serum engu-20μL noma isampula ye-plasma ukuze uyisampula ye-diluent, bese uxuba kahle.
7.Engeza isisombululo sesampula esingu-80μL ukuze uthole isampula yomthombo wekhadi.
8.Chofoza inkinobho ethi "standard test", ngemva kwemizuzu engu-15, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode / liphrinte imiphumela yokuhlolwa.
9.Bheka umyalelo we-Portable Immune Analyzer(WIZ-A101).

IMIPHUMELA YESIVIVINYO NOKUTOLIZWA

.Le datha engenhla isikhawu sereferensi esisungulwe kudatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhawu senkomba ngokubaluleka komtholampilo okufanelekile kwenani labantu kulesi sifunda.
.Ukugxila kwe-LH kuphakeme kunobubanzi bereferensi, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe.Ngempela okungavamile, kufanele kuhlanganise ukuhlonzwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela ebangeni lesithenjwa elisungulwe yile ndlela, futhi imiphumela ayiqhathanisi ngokuqondile nezinye izindlela.
.Ezinye izici zingaphinda zibangele amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

ISITOREJI NOKUZInza
1.Ikhithi iyimpilo yeshelufu yezinyanga eziyi-18 kusukela ngosuku lokukhiqizwa kwayo.Gcina amakhithi angasetshenzisiwe ku-2-30°C.UNGAQISHI.Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
2.Ungasivuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakama 40-90%) phakathi kwemizuzu engama-60 ngokushesha. ngangokunokwenzeka.
I-3.I-diluent yesampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.AKUKHO ukushintshanisa ama-reagents phakathi kwamakhithi ane-lot ehlukene No..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni.Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA.Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ngowenkomba yomtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli kuphela, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezimpawu zayo, umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi. .
.Lesi siphehli sisetshenziswa kuphela ekuhlolweni kwe-serum ne-plasma.Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.

IZIMPAWU ZOKUSEBENZA

IZIKHOMBISI
1.Hansen JH,et al.HAMA Ukuphazamisa Nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Imvelo Yama-Heterophilic Antibodies kanye Neqhaza Ekuphazamisekeni Kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

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