Ikhithi yokuxilonga ye-Estradiol(ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo.Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
I-Diagnostic Kit ye-Estradiol (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-Estradiol (E2) ku-serum yomuntu noma i-plasma, esetshenziselwa kakhulu ukuhlola amazinga e-Estradiol.Kuyisampula ewusizo yokuxilongwa kwe-reagent. kufanele kuqinisekiswe ngezinye izindlela.Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Estradiol(E2) iyi-hormone ebaluleke kakhulu futhi esebenza kakhulu ku-estrogen.Isisindo sayo samangqamuzana si-272.3 D.Ngokujwayelekile, kwabesifazane abangakhulelwe, i-E2 ikhiqizwa ikakhulukazi ngamaseli e-sheath nama-granular kanye namaseli e-luteal ngesikhathi sokuthuthukiswa kwe-follicular.Ngesikhathi sokukhulelwa, I-E2 ikhiqizwa kakhulu yi-placenta, kuyilapho amadoda ekhiqizwa ikakhulukazi ngamasende. Ngemva kokuthi i-E2 ingena egazini, i-1% kuya ku-3% ayibophi kumaprotheni, ama-40% abopha ku-hormone yocansi ebopha i-globulin(SHBG), kanti amanye abopha i-albumin, i-metabolise esuka esibindini ibe ama-sulfate ancibilikayo emanzini noma i-gluconaldehyde esters, futhi ikhishwe emchamweni.I-E2 iyinkomba ye-hormone ebalulekile yokuhlola ukusebenza kwesibeletho.

ISIMISO SENKQUBO
I-membrane yedivayisi yokuhlola ihlanganiswe ne-conjugate ye-BSA ne-Estradiol endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula.Iphedi lomaka limbozwe nge-fluorescence mark anti E2 antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula, i-E2 kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti E2, bese yenza ingxube yokuzivikela komzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula indawo yokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa ne-Estradiol kulwelwesi. Ukuhlushwa kwe-Estradiol kusampula kungatholwa nge-fluorescence immunoassay assay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:
.Iphepha lokuhlola ngalinye elifakwe esikhwameni nge-desiccant 25T
.Isixazululo 25T
.B isixazululo 1
.Faka iphakheji 1

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isitsha seqoqo lesampula, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Amasampula ahloliwe angaba i-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2.Ngokwezinqubo ezijwayelekile qoqa isampula.I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-7 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-6.
.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.

INQUBO YOKUHLOLA
Inqubo yokuhlola yensimbi bheka imanuwali ye-immunoanalyzer.Inqubo yokuhlola i-reagent imi kanje
1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
3.Khipha ikhadi lokuhlola esikhwameni se-foil.
4.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
5.Faka u-30μL we-serum noma isampula ye-plasma ku-A, bese uxuba kahle.
6.Faka isixazululo esingu-20μL B kule ngxube engenhla, bese uxuba kahle.
Shiya ingxube ukuze20imizuzu.
Engeza ingxube engu-80μL ukuze usampula kahle ikhadi.
Chofoza inkinobho “yokuhlola okuvamile”, ngemva kwemizuzu eyi-10, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).

IMIPHUMELA YESIVIVINYO NOKUTOLIZWA

.Le datha engenhla isikhawu sereferensi esisungulwe kudatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhawu senkomba ngokubaluleka komtholampilo okufanelekile kwenani labantu kulesi sifunda.
.I-concentration ye-Estradiol iphakeme kune-reference range, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe.Ngempela okungavamile, kufanele kuhlanganise ukuxilongwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela ebangeni lesithenjwa elisungulwe yile ndlela, futhi imiphumela ayiqhathanisi ngokuqondile nezinye izindlela.
.Ezinye izici zingaphinda zibangele amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

ISITOREJI NOKUZInza
1.Ikhithi iyimpilo yeshelufu yezinyanga eziyi-18 kusukela ngosuku lokukhiqizwa kwayo.Gcina amakhithi angasetshenzisiwe ku-2-30°C.UNGAQISHI.Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
2.Ungasivuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakama 40-90%) phakathi kwemizuzu engama-60 ngokushesha. ngangokunokwenzeka.
I-3.I-diluent yesampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.UNGAshintshisani ama-reagents phakathi kwamakhithi anenombolo ehlukile yenombolo..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni.Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA.Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emitholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli kuphela, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe kabanzi kuhlanganiswe nezimpawu zayo,
.umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Lesi siphehli sisetshenziswa kuphela ekuhlolweni kwe-serum ne-plasma.Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.
IZIMPAWU ZOKUSEBENZA

REFERENCES
1.Hansen JH,et al.HAMA Ukuphazamisa Nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Imvelo Yama-Heterophilic Antibodies kanye Neqhaza Ekuphazamisekeni Kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

I-Xiamen Wiz Biotech CO., LTD
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